2024-10-23 REL PDBe PDBe PDBe 2019-11-10 2019-11-27 2019-11-27 2024-10-23 Muhleip A Amunts A mitochondria, ATP synthase, MEMBRANE PROTEIN Muhleip A McComas SE Amunts A Elife US 8 2019 31738165 doi:10.7554/eLife.51179 2050-084X 6tdu FULLOVERLAP Euglena gracilis mitochondrial ATP synthase dimer Euglena gracilis mitochondrial ATP synthase dimer 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Euglena gracilis 2 ATPTB1 Euglena gracilis 0.056026699 2 LEVO MTAVVKNLAKLPAATSQILTNVSKLQTFHGLLEERRDKYAPLAYHTYDNLKQKTTWHPIAHAWVDEGLPVSKKEYNEYCW LKKDMQRLLPLASPFVFGIYGILPLAVWLSNDGYLPSAFSSKKDIVSKKLEWYSSYGDDLRQQVGPMLQHRLKRHLRGTL NNEHRLMLDEVTESYKEIFYSHYTGQLRDVRKCAHLRLYDGTSTVLLLTNKEPVELTSELLQKWNAIKAAKLSPEEEKKA RNEALIEAYKEQELHGGPHVKHMQGYGIPADTPLLGENAKGDQYTQPPESASIPLEQLEWTGDTVFIPAEYRTEMEDWGR ELTKLANQFLLLPWRFVSNAWNQRRLVSWFEEILQEDALIAKEGGVQALSDDELKVALLDRAVIRCDEELTRGDMEARYK EISWLMSLRNPFIVLAWQTGYYRSTYSPEDDLPEASILPKLNRTVLDVDVHNELAPDHPEKPLPRVHPALYPNSHLALAK EVAVLAK ATPTB6 Euglena gracilis 0.021737113 2 LEVO MTHAELHLFDLDEFMQTYKRLQTRQDWLIENKCKKSRLFSYVAAVIAFTVGKSATMSDEAILAKIDPYVTSEVRVQRGAW WRSGYFTKEEVEMMTPKGPIARYYKFLLGVRRFPLKHGALSWACGFVPAWLTFTSLNHWAQNRRLNRYLTQESVFGEMAR ELVRGKTADEATTSVMARVEKEILGVH ATPTB12 Euglena gracilis 0.011432077999999998 2 LEVO MSSYTGAALAPKSERLRLAFEEKQKDHQKCIEEAKGKGLKKDELIDACAWTHRKTILALKDWFAYRPPFQDRRSKWAEYC SIRHDSGSWLGWSQKFF ATP synthase subunit a Euglena gracilis 0.032666373 2 LEVO MLNSNIYIIIYGGIIMYSIMIIIQMFLYNFSNKIYIEVEINKYILSKNNIDIYWIICNCTIIIIITTLNHIINKIGIYNM IEYNICYWLIGTGLGLYISPFIVFGYKFFVYIMDLNNYSLNIYHNNNKMNDIQQIYNGTNYNDTMIFFIKDINNIFTIYR SINFFMNWLYQMIYYGVRMWLVFVLHSFSLGSFGELITVITDNNLIFNVFYIGLLGLGFILYLIVIFYLGIQIYVYISFS LSFLHSTILLFLVNYIPHYNNKSIFNTFTNKSIY ATP synthase subunit b Euglena gracilis 0.012673109 2 LEVO MPSTSPADKDVPMSILHTHGLSYVNWCMSLAPGLLVFEGFFRARYYRSRVPPSRTVLMNGLKMRMFSLARQQAPKIVHKP VLSPIPEHLRLVKNVAQVQIDMLKLLNAQAAK ATP synthase subunit d Euglena gracilis 0.053928289 2 LEVO MMRRACRIIRPSHVRGVSGVAPTIYLRSKAALPATSTTDVRPQLYALQRFAKAQLKTATEAERAAIEADIARYQEYLDSD LEKLKQDVAEDTAKKQKLIPLLDRYPDVPIEKIPEHANVLLKKIDACLEILSKDIGEVTDAEAHEMYFETSKFQILHIYT GCVASFPEGDVPPGAVECLPGQVIRTKVNGEDVMLEIDEVDPGYQVCWFKPDVPLPENAEILWSYPYEPTAALPTGTTWE EGQANVLIPAEPTPEAAVWPPTPVTNVYAPMAEKLALKSNPELKVLFKEALLQPAKLLPLDVDYQCSHDREVVEAKRDRY LTALVEAEQAPPLPFTPDVLQLQLEHNVLKGELIDRLRALEYTIVTEQLQARLHERRLRGDVIDEWEELDYHPLVRDDTY LAIDFGDPTFGRYIWKLFPHTDGDEECMFKDTRLDVLPPQVNPLNAILAQHTAQTPVHRSLEKRLWTEVRATAVSE ATP synthase subunit f Euglena gracilis 0.011193877 2 LEVO MAPLYPVLSQASLYKRHFFKNIKLFHVVFYVGAPCVTFGTAAWSGSNRNSREAIFMVIEERHGWDNFKKLSSHQQGVIMQ EAAQESLLARNKGELHLP ATP synthase subunit i/j Euglena gracilis 0.012529404 2 LEVO MVYTNWQSSYTRLFVSKPWMWHPLAWMTLSVGIWWKFGKESLCNERSFYIHTHPKWAPHKFHTVYNWSRDPIKWTLAEQY ASIIRNTNTDIEAVLKIKLPANAN ATP synthase subunit k Euglena gracilis 0.012739870999999998 2 LEVO MAFGRTRPTLSSPLVPVWNDLRALQVFTSQEYMQKRGPGFTNTLEYKLSCLNPVKWYDMMKVMPGGKAFVGTALGLALFG GWGVEFVKNISVMTKEKPPIDWNNEKLGHLTRS ATP synthase subunit 8 Euglena gracilis 0.007011208 2 LEVO LIPVSLVDLININIIFYILLLYTLLLFFIPLFLASINYTYHYIYKYYNYNYNFINNN ATPEG1 Euglena gracilis 0.019738803 2 LEVO MSLAKVWMYASWIPRGIPKAMANELSSAAAALAHPEAIARVAQLESQGKNPYRVARAEFWQMYLACWPYRFRNTVVEWET CKAKVLKGSVDLQDIVDLLYLLAWAYLFWILGEIYGRGSLYGYRFDGEIHRQEAQNVILYKEKEAQEMAVVMEKLEKEIQ EWLKTMEQE ATPEG2 Euglena gracilis 0.016210519 2 LEVO MPLPNAVVQGYTSVRGPKRPLDHFYGRTPLNIDTLWHWVKFPHRYDNLRFAVCFWAFLVSAHFANKKQRNLRVEWEKNME IQKKLHPSGLWSEEQAFAAAEKLGRPKAGHPMRVFEDGYQQFDLKPKLFDPDEEAHH ATPEG3 Euglena gracilis 0.013807529 2 LEVO MADHNKKDVGSWASPNEHLMFFDFSSWLLVDFGKRWERWVSFKKSFLTTTRSPYWSPQFFLLTFFQLRNSNVKLCENWNW APKGDDFNLLHNSAAEPFGRDLKAHLEREAGAKHHH ATPEG4 Euglena gracilis 0.013703778 2 LEVO MGGDAHAAPAEKPDPALDATKALPKALEEVEFFQSYAVRRKTGFHLFNRATGSPTIVGPMFYNLYNFVRIGRVSKYVCWL SLPLVFQRMWMKNRATGMEYDIDLENYAPFEAKKNPMHGH ATPEG5 Euglena gracilis 0.010825277 2 LEVO MSLPTFFLAPPLTEPFRARHRRKCIEKKDTFFQCLDKNKNETEPCEVEASGYSQECPDSWRRYFNEQRWKALELKGEVKH ANLKNTQQHF ATPEG6 Euglena gracilis 0.009236822 2 LEVO MFGVTRKLLGELSEYVEVNEKGMPKPQALSLWNMPYAKRRALTKFARGVRWQFIVLFIALYNFKNRDDSHLLRRGAYN ATPEG7 Euglena gracilis 0.008861477 2 LEVO MMRISRKLLVPVANFRPKKPWDGPWGIQISQKKDRPFIAMWILFPLLLVDHLTREYYAYWHSSKVPVTDVFGDF ATPEG8 Euglena gracilis 0.0074417879999999995 2 LEVO MGGKASEAVTIAFRFPHRTTFLVKQNVGQKLNKGHQTFWQLVAGGWLFFLLINRTSFKPKLAAPKV ATP synthase subunit alpha Euglena gracilis 0.061897199 6 LEVO MHYAEAATPAKSAGSAVAKKHLEAKQKIGFVHGIDGTIATIAPAASKVTVPYNTVLEIAVSATNIANALVFNLEKDGSIG VILLDNISEVRSGQDVYATGSLLKIPVGFHMLGKIINPLGKEIPTGLFTKAAPLLDDTKLGLVEEMAPNIVSRQPVNYNL LTGYKVIDTLIPVGRGQRELILGDRQTGKTSIALSTILNQTKVNNEILSKNNVLSVYVSIGQRCSNVARIHRLLTEYDAM KYCTIVAATAADPAGLQYLAPYAGTTLGEEFRNSGRHILLVYDDLSKQAVSYRQISLLLRRPPGREAYPGDVFYLHSRLL ERSAMMSPQKGSGSLTSLPIVETLSNDVTAYIVTNVISITDGQIYLDAKLFTGGQRPAVNIGLSVSRVGSSAQNKAMKKV GGALKMLMGEYRKMAGEQTSGSQNVSPVMIRGARCLQLFNQKGPSYFMDAIVALYAVTNGYMDDVKLQYSKFYEFLLLNK DLPVLYGQVNNKYFYMYNKNLNYFIRYFGLNHEILEPELKKYIEIHTNLFLDNYQSRMNELKSDEDLVQLKNLLYACKRT V ATP synthase subunit beta Euglena gracilis 0.053991066 6 LEVO MVGRMMATAPATAADVKQVGYVQQIIGAVVDVTFTDSVPPVLTALTVDAKETGTLLTMEIVQHLDTKTARCICMSSTDML RLRTPVVNTGSQITVPVGEATLGRIFNVMGDAIDQRGPVKNKVRWPIHRKAPTLAEQSGKDEVLVTGIKVIDLILPYCKG GKIGLFGGAGVGKTVIIMELINNVAKGHGGYSVFAGVGERTREGTDLYLEMMGSKVIDLQGDSKCVLVYGQMNEPPGARA RVAQTALTMAEYFRDEAGQDVLLFVDNVFRFTQANSEVSALLGRIPAAVGYQPTLAEDLGMLQERITSTVKGSITSVQAV YVPADDITDPAPATTFSHLDATTVLSRSVAEAGIYPAVEPLECASRIMDPDAIDVNHYNVAMDIVEMLTKYKELQDIIAV LGIDELSEEDKLIVDRARKVAKFMSQPFAVAEVFTGMKGYYVQLEDCVSDFGSLLMGQCDNIPEMAFYMVGGLDSVKEKA AKMAAEAAAMRERARKAAEAK ATP synthase subunit gamma Euglena gracilis 0.035110375 2 LEVO MPGGGTIRFWREKLEGYKKYHQIVKTIKMVTLAKYRQTVVRTRVRDQTLRYTRKALDAKTQDDQEVIEKSECLLYVPITT NRGSCGALNTNMVRYLQEVENPKMTIISVGKKALDAMTKVFQDTYRRTILNDMKQAMSFQFAAYVLEHMNTVPWDRAQIV YNRYHGAASQKLAIFNLPKFEDWKQKLEEDSAGDGKIEEDGLLQSLPMKTALGELEETAVEDFYNFHSCLAVLNAVSENE LSEYAARIVAVENQLGNITGLMQLADYTYNKTRKELITAELLEIIGTMTAMHAGKKVGLKKTEFWK ATP synthase subunit delta Euglena gracilis 0.019559953 2 LEVO MRASRTLLLSVSRFMRQDPRKFFPDNGFRFFDGPEDSFGDGNIPAQIILTLTRQDEFILKQEPVAAITIRTNEGEMGVLA GHEYTVQQLAPGILEVEYEGGKKDQYVISGGFAHVNDTGVVDINTVEAVPLEEIDHEKLAKALEEARAKSQSPDEAVRIQ GEIALEIFEPLEAALH ATP synthase subunit epsilon Euglena gracilis 0.008751980999999999 2 LEVO MSWRDAGISYLRYLSIVTRCIHEVQKEGPLLTKNVRFSTIGWKSLYLDHGATKEYTAIPAELEKIPENQVAQQHHA p18 Euglena gracilis 0.020995094 6 LEVO MQKLSRVVCNRLVRFHGTVAASAGGKRYDLFGYEVSVATGPFIEEIKKAQFYDDAGEVIVKMNLANTPPDLQTYNAVLER ILNCKSKRSQPVKGENKFAAMMDILEEMDARSGIKPNAESWGYVLKELVQAGDFRLGWVCIAGMKSLGITPDQALVDANE ANAAKAKAAGTDFPAYLKKAAPESFDTKAWGI oligomycin sensitivity conferring protein (OSCP) Euglena gracilis 0.02956452 2 LEVO MHMRRAVSVFGRCRSLNGLRNYAVPSPKYIEIYQSDFSRNAYPLELLGGSHVDFAKLLYSFADQVENKKFEVYVEDFKKL DSIIAEKGPFWAEEKIFQSPTFQGLSEGFKFILGWIQSEGAIDRLENVRLAYKELVNEARKETTATVIVAKEPSGNDLAE IRKQVEELHKESPLKDYKLVLETKVDPSIGGGYILEVCNQVVNRSAAAAAAETAALAKASAAQVDWTSLPAAPPRPSPSA PDTLIRLLGSVVDDLADADKVEQKYGA inhibitor of F1 (IF1) Euglena gracilis 0.011687415 2 LEVO MAAACAVRGFTTARPMLTPNKVKVPGRKPQDEEDLTWAEADRKLTPEERYARDKQMALLDKMTSQVEELEKSHTEQKKSN KGVKAQIEAISRQLEALKAQLKE ATP synthase subunit c Euglena gracilis 0.010820831 20 LEVO MQRGSSITKVVRRAALARSTRNAAIAYEVTVNGANLIGAGMAASGVGVPAIGVAMCFSSYMLAAARQPNMSAKLLPYCIL GFALSEALALFTLLIALLELFVFS ATPTB3 Euglena gracilis 0.035175836 2 LEVO MAAKTIPFVASTALGERLFAGVQKVLAAAKAPVSLVQTDAKFAPADAKYLLAEPLSAAATGELANKYGLSSKVTSGAASQ FYPNSIYPSLNVEVVQNLYALSNPAFSSLSATTTKAVTVKDESAIKLAELQKETERNISSFFRDEANKSVQATLRLACDK AIKTKADKKTVMVVTKPHGDAFDDLLAQVTKSESDGRADELRNSSVSVEPTLVGNAWPKLVMFPEGVNVVVCGPNASGDQ VAQLFVGIAGGTGMVAQQLVGDAVVFTSANAEDNENPTGALLAASNLLTALGHEAEAKKIAAAVAKAYTTDRILPKELPG GKADLEAFIDAVAKHASA ATPTB4 Euglena gracilis 0.018833509999999998 2 LEVO MFRGFRPVLAADAVKFQTLYNVLTGKQHLKDQVPVKDCNLTAIFGASWKADLNKWFDSEYAPKLPAAERDSAKKSLDLYL KRVDLTRYTREELTTYGILACGPGKVDALTEKHLLETGKARLEELTAGLGNKDEGVNAFRKEVEQEGKYANWPAEKSKAL ADKVIAASP CARDIOLIPIN 0.0014640429999999997 25 CDL DODECYL-BETA-D-MALTOSIDE 0.000510615 8 LMT 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE 0.000648891 12 LPP FRAGMENT OF TRITON X-100 0.00035250799999999997 18 TRT ADENOSINE-5'-TRIPHOSPHATE 0.000507181 8 ATP MAGNESIUM ION 2.4305e-05 10 MG ADENOSINE-5'-DIPHOSPHATE 0.000427201 2 ADP singleParticle particle 7.4 Quantifoil R1.2/1.3 COPPER 300 GLOW DISCHARGE 30 ETHANE 100 277 FEI VITROBOT MARK IV

3 seconds blot.

FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20 GATAN K2 QUANTUM (4k x 4k) COUNTING 9045 10.0 36.3 1 555269 EMD-3559 C2 FOURIER SPACE 4.32 FSC 0.143 CUT-OFF RELION 27232 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION RELION emd_10467_msk_1.map emd_10467_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 461.99997 461.99997 461.99997 90.0 90.0 90.0 X Y Z -0.019922903 0.043254424 0.00041869594 0.0029265024 1.05 1.05 1.05 AUTHOR ::::EMDATABANK.org::::EMD-10467:::: emd_10467_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 461.99997 461.99997 461.99997 90.0 90.0 90.0 X Y Z -0.020641547 0.0411167 0.0004099282 0.0029506756 1.05 1.05 1.05 AUTHOR ::::EMDATABANK.org::::EMD-10467::::