2021-10-06 REL PDBe PDBe PDBe 2019-11-09 2020-11-18 2020-11-18 2021-10-06 Swedish Research Council 2012.0112 Sweden Knut and Alice Wallenberg Foundation Sweden Cryo-ET on GroEL Zou XD Zhao JJ Xu HY Carroni M Zhao J Xu H Lebrette H Carroni M Taberman H Hogbom M Zou X A simple pressure-assisted method for MicroED specimen preparation. Nat Commun UK 12 5036 5036 2021 34413316 doi:10.1038/s41467-021-25335-7 2041-1723 EMD-10013 other EM volume EMD-10014 other EM volume EMD-10015 other EM volume EMD-10012 other EM volume EMD-10417 other EM volume EMD-10466 associated EM volume GroEL GroEL 0 Escherichia coli K-12 0.58 tomography particle 0.70 7.4 Quantifoil R2/2 COPPER 300 CARBON CONTINUOUS 0.2 GLOW DISCHARGE ETHANE
A new freezing method was used, called Preassis.
The GroEL grid for cryoET data collection was prepared by a new specimen preparation method, called Preassis. A Quantifoil continuous carbon grid was used.
NO SECTIONING FEI TITAN KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 GIF Bioquantum 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 1.4 1 50 emd_10466.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 960 928 175 17 -16 88 960 928 175 13248.0 12806.4 2415.0 90.0 90.0 90.0 X Y Z -2171.0 2096.0 105.888145 116.60059 13.8 13.8 13.8 AUTHOR Reconstructed tomography data.The GroEL specimen preparation method is Preassis, and a Quantifoil continuous carbon film grid (R2/2) was used. In the hole of this tomogram, the carbon film was broken. emd_10466_additional_1.map.gz 1 IMAGE STORED AS SIGNED BYTE 934 862 125 5 -45 38 934 862 125 12889.2 11895.601 1725.0 90.0 90.0 90.0 X Y Z -128.0 127.0 -0.819301 25.19082 13.8 13.8 13.8 AUTHOR Reconstructed tomography data.The GroEL specimen preparation method is Preassis, and a Quantifoil continuous carbon film grid (R2/2) was used. In the hole of this tomogram, the carbon film was intact. emd_10466_additional_2.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 3708 3838 51 0 0 0 3708 3838 51 51170.402 52964.402 703.8 90.0 90.0 90.0 X Y Z 0.0 565.0 124.88349 48.74973 13.8 13.8 13.8 AUTHOR Raw tomography data. The GroEL specimen preparation method is Preassis, and a Quantifoil continuous carbon film grid (R2/2) was used. In the hole of this tomogram, the carbon film was intact. emd_10466_additional_3.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 3708 3838 51 0 0 0 3708 3838 51 51170.402 52964.402 703.8 90.0 90.0 90.0 X Y Z 0.0 591.0 121.87589 49.24713 13.8 13.8 13.8 AUTHOR Raw tomography data. The GroEL specimen preparation method is Preassis, and a Quantifoil continuous carbon film grid (R2/2) was used. In the hole of this tomogram, the carbon film was broken.