REL PDBe PDBe PDBe 2003-04-15 2003-05-12 2004-01-06 2012-10-24 Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process. Valle M Sengupta J Swami NK Grassucci RA Burkhardt N Nierhaus KH Agrawal RK Frank J Valle M Sengupta J Swami NK Grassucci RA Burkhardt N Nierhaus KH Agrawal RK Frank J Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process. EMBO J. 21 3557 3567 2002 12093756 doi:10.1093/emboj/cdf326 1ls2 FULLOVERLAP 1lu3 FULLOVERLAP 70S aa-tRNA-EF-Tu.GDP.kirromycin complex from E.coli 70S aa-tRNA-EF-Tu.GDP.kirromycin complex from E.coli 6 2.5 2.5 E.coli 70S ribosome
10 pmol
false Escherichia coli ribosome-prokaryote: ALL aa-tRNA Escherichia coli
14 pmol; fMet-tRNA(fMet) at the P site and Phe-tRNA(phe) at the A site
TRANSFER DOUBLE HELIX false EF-Tu Escherichia coli
20 pmol
false GDP synthetic construct
8 ul of 2.5mM; [Alpha-(32)P]GTP The radioactivity contributed by the Alpha-(32)P moiety of the GDP served to calculate a ~50% occupancy for the elongation factor.
false kirromycin synthetic construct
3 ul of 100 mM; antibiotic
false MF-mRNA Escherichia coli
15 pmol; M(Met).F(Phe)-mRNA
false singleParticle particle 7.5
20 mM HEPES-KOH, 6mM MgCl2, 150mM NH4Cl, 4mM 2-metcaptoethanol, 0.05 mM spermine and 2 mM spermidine
 ETHANE 30 93 HOMEMADE PLUNGER
Vitrification instrument: two side blotting plunger
Blot for 2 seconds before plunging FEI/PHILIPS EM420 FLOOD BEAM BRIGHT FIELD LAB6 100 2.0 1.041 3.844 49000.0 52000.0 GATAN LIQUID NITROGEN 93
Microscope: Philips EM420 Imaging date: 1997
1998-06-01 KODAK SO-163 FILM ZEISS SCAI 14 45 10 1.2 12. Cryo transfer
defocus groups
 C1 OTHER 16.8 FSC 0.5 CUT-OFF SPIDER
Final map was calculated from 8 CTF corrected defocus groups
7985
SPIDER definition
 2 emd_1045.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 136 136 136 -68 -68 -68 136 136 136 365.84 365.84 365.84 90 90 90 X Y Z -82.174800000000005 184.76400000000001 4.36412 20.063500000000001 2.69 2.69 2.69 48.299999999999997 70S aa-tRNA-EF-Tu.GDP.kirromycin complex
::::EMDATABANK.org::::EMD-1045::::
 1EFC RIGID BODY FIT manual
Protocol: Rigid Body. The EF-Tu domains were separately fitted by manual docking using program O (pdb codes for fitted coordinates: 1LS2, 1LU3)
cross correlation coefficient