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    <admin>
        <current_status>
            <date>2021-04-14</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-10-31</deposition>
            <header_release>2019-12-11</header_release>
            <map_release>2019-12-11</map_release>
            <update>2021-04-14</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Seneca Foundation</funding_body>
                <country>Spain</country>
            </grant_reference>
        </grant_support>
        <title>Example of tomogram used for in situ template-free membrane bound complexes determination</title>
        <authors_list>
            <author>Martinez-Sanchez A</author>
            <author>Lucic V</author>
            <author>Chakraborty S</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Martinez-Sanchez A</author>
                    <author order="2">Kochovski Z</author>
                    <author order="3">Laugks U</author>
                    <author order="4">Meyer Zum Alten Borgloh J</author>
                    <author order="5">Chakraborty S</author>
                    <author order="6">Pfeffer S</author>
                    <author order="7">Baumeister W</author>
                    <author order="8">Lucic V</author>
                    <title>Template-free detection and classification of membrane-bound complexes in cryo-electron tomograms.</title>
                    <journal_abbreviation>Nat.Methods</journal_abbreviation>
                    <country>US</country>
                    <volume>17</volume>
                    <first_page>209</first_page>
                    <last_page>216</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">31907446</external_references>
                    <external_references type="DOI">doi:10.1038/s41592-019-0675-5</external_references>
                    <external_references type="ISSN">1548-7105</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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            <emdb_reference>
                <emdb_id>EMD-10432</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Density map obtained from this sample</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-10433</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Density map obtained from this sample</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-10434</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Density map obtained from this sample</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-10435</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Density map obtained from this sample</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-13436</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Density map obtained from this sample</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-10437</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>Density map obtained from this sample</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-10439</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
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    <sample>
        <name>Intact P19 cells</name>
        <supramolecule_list>
            <cell_supramolecule supramolecule_id="1">
                <name>Intact P19 cells</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                    <tissue>teratocarcinoma</tissue>
                </natural_source>
            </cell_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>cell</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE-PROPANE</cryogen_name>
                        <chamber_humidity units="percentage">90</chamber_humidity>
                        <chamber_temperature units="K">310.15</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                    <sectioning>
                        <other_sectioning>NO SECTIONING</other_sectioning>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>OTHER</illumination_mode>
                    <imaging_mode>OTHER</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">70.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">0.2</nominal_defocus_min>
                    <nominal_defocus_max units="µm">0.25</nominal_defocus_max>
                    <nominal_magnification>42000.0</nominal_magnification>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <basic />
                    </alignment_procedure>
                    <specialist_optics>
                        <phase_plate>VOLTA PHASE PLATE</phase_plate>
                        <energy_filter>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">1.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <algorithm>BACK PROJECTION</algorithm>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                    <details>Original reconstructed pixel size is 3.42 Angstroms, however due to size restriction a 4-times binned version with 13.68 Angstroms per pixel is provided here</details>
                    <number_images_used>60</number_images_used>
                </final_reconstruction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_10439.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
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        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>928</col>
            <row>928</row>
            <sec>500</sec>
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            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>928</x>
            <y>928</y>
            <z>500</z>
        </spacing>
        <cell>
            <a units="Å">12695.04</a>
            <b units="Å">12695.04</b>
            <c units="Å">6840.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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            <maximum>3570.2153</maximum>
            <average>67.23514</average>
            <std>257.27914</std>
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        <pixel_spacing>
            <x units="Å">13.68</x>
            <y units="Å">13.68</y>
            <z units="Å">13.68</z>
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            <contour primary="true">
                <source>AUTHOR</source>
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        <label>::::EMDATABANK.org::::EMD-10439::::</label>
        <annotation_details>Example of tomogram used for in situ template-free membrane bound complexes determination</annotation_details>
    </map>
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