<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_9_3/emdb.xsd" version="3.0.9.3" emdb_id="EMD-10438">
    <admin>
        <current_status>
            <date>2024-05-22</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-10-31</deposition>
            <header_release>2020-01-29</header_release>
            <map_release>2020-01-29</map_release>
            <update>2024-05-22</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>French National Research Agency</funding_body>
                <code>ANR-15-CE11-0022-01</code>
                <country>France</country>
            </grant_reference>
            <grant_reference>
                <funding_body>French National Research Agency</funding_body>
                <code>ANR-10-LABX-0030-INRT</code>
                <country>France</country>
            </grant_reference>
            <grant_reference>
                <funding_body>French National Research Agency</funding_body>
                <code>ANR-10-IDEX-0002-02</code>
                <country>France</country>
            </grant_reference>
        </grant_support>
        <title>SAGA bound to TBP - whole structure</title>
        <authors_list>
            <author>Papai G</author>
            <author>Frechard A</author>
        </authors_list>
        <keywords>Transcriptional co-activator, Histone-acetylation, TRANSCRIPTION</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Papai G</author>
                    <author order="2">Frechard A</author>
                    <author order="3">Kolesnikova O</author>
                    <author order="4">Crucifix C</author>
                    <author order="5">Schultz P</author>
                    <author order="6">Ben-Shem A</author>
                    <title>Structure of SAGA and mechanism of TBP deposition on gene promoters.</title>
                    <journal_abbreviation>Nature</journal_abbreviation>
                    <country>UK</country>
                    <volume>577</volume>
                    <first_page>711</first_page>
                    <last_page>716</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">31969704</external_references>
                    <external_references type="DOI">doi:10.1038/s41586-020-1944-2</external_references>
                    <external_references type="ISSN">1476-4687</external_references>
                    <external_references type="CSD">0006</external_references>
                    <external_references type="ASTM">NATUAS</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6tb4</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>SAGA bound to TBP</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>SAGA bound to TBP</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>8</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>10</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>11</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>12</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>13</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <molecular_weight>
                    <theoretical units="MDa">1.6</theoretical>
                </molecular_weight>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>TATA-box Binding Protein (TBP)</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii GS115</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>SAGA complex</name>
                <parent>1</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>8</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>10</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>11</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>12</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>13</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii GS115</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>TATA-box Binding Protein (TBP)</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.026931299</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="469008">Escherichia coli BL21(DE3)</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MDSLKLPTTAAQAKAFTSSGSLSFPMNNDDMIKADPSQTEQHIIKQDIEPEIKDVVVSDDANSSGIVPTLQNIVATVTLG
CRLDLKTVALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKIGFAAKFTDFKI
QNIVGSCDVKFPIRLEGLAFSHGTFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYQAFEAIYPVLSEF
RKM</string>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Transcriptional coactivator HFI1/ADA1</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.049888273</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MASTELVASTPMPSRNGQGSHQKLNGGNVNSATNTPTVASTPVNQPRKLPSENFSGLGGAIRIEIEPLVVDFQRRLGKNW
GRYQIAVSLFLVGKLSRRELVDELDDILDRNTVKMHNQLLLGNLANSLKESAGDRVGSGGFGGSMFNKRVKDSRKSSQYE
RLKRDILALPVRERRRIKAITRESGKKGMVNSVITQTRQALIPKVPVVTRPTNVPGNSVQWAQDVIHGFQALLASEIYEL
PEMDNLRTRMTGISREHGLIGPVDDSVIEIMLIGLEQHLKGIVEAAIDIVKYRRTKYTNNSVVDTLQKEQTNTSDTSSSK
VSRKRDRHTLTIEDMYDTLEQYPYLVEPGGTLLRLQSVMLHDEDELSDDEILKHILKPRTSDSTDRKPKQLQENGTADSL
SKVTKSITNGANASGDAVPNGKAEHIQNTMGTKEELNWFIYDILSNNE</string>
                    <external_references type="UNIPROTKB">C4QZA3</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>SAGA-associated factor 73 (Sgf73)</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii GS115</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.079995227</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MQNPERPVVWKELGQYLDTLQPIHQEKPSSLPDLPSPSYKDSLSNPLRYRICNNCDRPILQSCLSKHIKICNQIVKVEKL
EEDDKPMNMVRKRKNQVLEESKESTPVTNTNSTNSSNAINSTTSKKTKKVKLPKEKKPKKERVKSTAKPKGPVDVERQCG
VPLPNGGYCARSLTCKTHSMGAKRAVPGRSAPYDQLLVAYQRKNQAKFAANAAAAHAARDDLIHGSQIPIDEDDEAHQVL
DGVAKSYPLPLERKVIMPARSRSSFLRMREMFAGAILPRVPTNPFGNLYSRTAVIDVDRAGDYYFSVRASPRMPNQQQNK
TQKNPQPPRPQVQQQQQAQKSQQSPPQAQQQISQSAGQQTSQTVQRLVQQQQRLQQQRQQTQIQQQQQHQQQQQQQQHQQ
QQQQQQQQQQQQTHMLQQQQQQQQQQQMLLLQQQQQQQAQQQQSPPITHQSPIPDNRPSNQLPQQYSQMTPQQLQLQQQL
QLQQKQKEQFRLQQQQHLRNL(UNK)MR(UNK)TPQQQQQFANLTPEKRAQFQRMQLVQLQQQIKNQAIQNQMQQQQQQP
SHVQSQAQTQFQQAQLKAQSRAQVLQAAQRQMQNSPTSPVTSMSSPLAIPSNLANQGPNQTSQLPNEQLSNPLMGNQFQG
QFNQYQPNYRENLEGGGGSMDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPGGSHHHHHH</string>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="4">
                <name>Spt20</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.059530578</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSQIQSSQVGAKNMQTAQPQAQQRPINGSVTLSNGQRINPQNLTPQQQRLLQQKVLHQKLKNYKFAETSEEILHKYEKFP
PSLTFHIHENHYRFGDQDGVIPKNSSVIKAFLEYVAREEIPPALIEVTKDAGVQLYEGCIILRIYDHRHLVAHINDAKDS
QHVSSESSANNTNNEKKEPSKVPKTYTTILRPTQLSLYADLLYQTDYLQVRFTDSLSLNIETEILTASKRNLDLSVPLNP
HRATANLKPEVKYPYYDPETDTVVHEHRPDARGNMVNADTVDYRKLHEDMAQHGSDYERLMLLLSDRFDDDSQTQGSSVT
SSQFMRLRFVEAWRKKEERLQESGGSSQRQRPLQPGQIPGMLSSFSSNTGNMNTMLDNLTPQQKAAIQQRMIQQGRAQPQ
QQQPDQEQLQEQQQQQPQQAMSQQRQQQLQQLYQRQQQHNQHHQQNQDELSSLDDKKIKKPPAKKQKKMTKKEMTTLHQN
VAGKTIPSAESTPPTTAANKKRGTYKKKNKYILIIND</string>
                    <external_references type="UNIPROTKB">C4QZ05</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="5">
                <name>Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.039030105</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTDAKDKDHKYRYRMEIQQMMFVSGETNDPPVETTSLIEDIVRSQVVEIVLHSSQTALSRGTKSIVPEDVIFLIRHDKAK
VNRLRTYLSWKDVRKNAKDQQSGDLADIGGDDLLDNSTSQVAASGAPGAPGSSSSTSMDSKMLSKYKKSKIRLPWELQFV
FTEQPLDTNEDDAEDEDERAATLASLKRLKTNDERTKNMTKEEYVHWSECRQASFTFRKAKRFREWCGLSHLAESRPSDD
VIDILGFLTFEMVCSITEEALIVKMLEEQNGDLASSTTTIQKLQESHRKRKHLFDGPDKDVRPITSGHVLEAWRRLQKRN
VEKKAIRNFQGGKLRSRVQLI</string>
                    <external_references type="UNIPROTKB">C4R3E4</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="6">
                <name>Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.136768828</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MANERALQYFVTSDNQRLYDLAAKLFSLGFFNCYFTPQQFQLLSVILDNGKLRIEEDGFVSTVWDEFIKGRLVLQFSKGD
YEKESTKHTVDNVKGTDDKTGIEVSKENGHSSDPPSADHDITESDNVGREETLDDQFSELYLKDGVSTSQFMCAKLRYLL
FEQAIDYLYTNSSMNGDAEEYSLLQNVDDMNEEKEETAKPATPQNVREIDNDYDDDEDEDEEDEEIKEASSKNPFKTEDN
EPLTNERQPTNNVFIRLCSDEKSLSKSLTLNLLTSMVTSKPEYPVEQLKGSEVIGTSHPLLGTASAIESKLEKRNEARLI
KNFSKIYHQFDKDERNFLKRRKLEISNKQFLDQDNNDPKDRPSSNSTSDEESNSNNLEKTSQPSSVNKLMQLGGAANLSL
KNLLSKVEENREKLNLSDLDLRSLIMDVRKNRSKWASDDKIGQEELYEACEKVVLELRGYTEHSTAFLNRVSKREAPNYY
QIIKKPMDLNTVMKKLKSFQYKSKQEFVDDLMLIWKNCLTYNSDPSHFIRVHAVAMQKKTLSLIPLIPDIVIRDRSELEK
DEVENVVETPQSSSIGTPMTTRVAGGKGPKKGRTHREPPTRPETPDGIAGEDSSQVSKAEQDSPAKALEVTENDSTKEKE
KDLSDVPTQEQKNDKTEKDDDYQEAVEDEEEDDDDNTNLEEDSTEDEDDLEVQTWNSLTSNVRYKICEKRRNLFKENKIQ
PNEEAIFRDTYQMENFMHYLGDDVRIVLNTPMSRYQYYDGNEDPYLIEYDISGGIPGLKYSGVSPDEGDLEDNMLVDQLM
KGESSIRESGFARKPTGMNKKFNEIIHLMQQIRRICFKISLIRQMQTQQFLHHTQMKPPDINEIQDIDIDQLSNLPNRDK
LDSDVSYNSLKRSISKILMANGFESTAPFCSDVITQIAENYFGNLVKTLKNHIESKSINKVMGDAKFQKVSNKDILLLSL
LENGVESPDALYEYYNENIVKQISKLEGLKSRLSSFLAELLRPGLQDLNERQFNDNSDEFLTGNFSSEIGDDFFGFRELG
LEKEFGLLTSTVPLHLLHSRLNSSIFNSNHEVSQLKFDDIQAEEKEQLYKREIPQHIKLIQPFLYNIAEKSKTIHYKQLK
KLNELQKMPENDDDILLIEDEDLPLKQRNTRPRVPPTGKIPNVKKKAVNGAYFLDPAIFSSNNQVKVEGDT</string>
                    <external_references type="UNIPROTKB">C4R5C7</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="7">
                <name>Transcription initiation factor TFIID subunit 10</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.023879145</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSTEKLFEDLDEDSLMQDVEGGVEGSMTIEEEPEPNDEKEAPHISMPSLPEYTRKDKTLEEILEMMEDEEFTPIIPDAVT
DYYLAKNGFETSDIKIKRILALATQKFISDIAQDAYEYSRIRSSSSVYTSANPQARARQLVAGQQQQQQQQPQQQQQQQP
QTGASQPAPTVGGSGGGGGGGGGTSTGTNNKVVLTMDDLRSALGEYGINVKRPNFYR</string>
                    <external_references type="UNIPROTKB">C4QXP2</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="8">
                <name>Subunit (61/68 kDa) of TFIID and SAGA complexes</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.066690133</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MNQPPNQPQDNDSQVSSSGMQGGNSMNYSSSAPVNGAGQGPQPQQNSQGQNGKKPVGMQQAQIQLLARRYQLEMQKAHQL
GPQTPQGAEHLQTATKIKHVLLSYQQQRQRQQGQVQGQGLSQPQGQNQAQGRVQQQTQGLSPDPGSHQGSPQFQQPHQVM
MGSAQTAGTSIANPQAQSNISSQVSQMAAAKVNSASPPIPPKTVGTPTGTPVGTQPRGQVTIQQFQQVKSILEEFEKKLR
TIEAAKRDQNLPEETLQKLLRQEAILKQRYAQTKATAYQMSQHLQRQQQALRAASAESAEVYVTGSASSPNMNVYNQQIL
TQQPQQQLQQQQLHQPQPQQPQPARQSPHLLIHQQQQQQQQQQQQQQQQQQHPVTHRPSNVSQTMPQPSQPLQSSTPSSA
VGRNQSPGATPVTSVNAAAKPSPGTSSTSTLINQHILKPAPPPTEIPARLQVKPPQPAAMKIPNRPTLLGGSAISQPSLT
TPVSIRPPPLEMEGDHVLQKRKLKELLRNVGADEGDGETVIDGDVEELLLDLADEFVTSVTSFACRLAKHRKVDNIDMRD
VQLHLERNWNIRVPGYASDEIRSVRKFQPTAGYNQKVQGVAISKSVNKN</string>
                    <external_references type="UNIPROTKB">C4R150</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="9">
                <name>Subunit (90 kDa) of TFIID and SAGA complexes</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.080933008</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MKQEPGDSSDKSAPSGSITPQPKPPQQANVSNQQPQKPQQFSAADLNRIVLEYLNKKGYHKTESMLRMESSHIPAPPTNI
PTPQTTNISRPTAPGRAPEYSDDPATIKRGYSILKSWCESSLDFYRPELEKFLYPVFVHCYLDLIARGYPSHAREFYDKF
SKDHSVLHEYEISKLGGISLKEHLQENDVAKIFRSHKFKVLIGRTTFNLLLYFLNENDAVGGGVVLRLINQYIEPVITTE
AIAVEREGELNLSEGIVELHTLNNTSIGGEQREISSVDAFNKKPVKLGKLQVDPEYSKELEAELKLKDEHEQAAQKKVST
TLLEEYRENFKVDPSDENNPSKDTLPLPLKSAQDLRNDIAMIQDSRAKIKLSAAQASLPSVCMYTFHNTNNDLTCLKFND
DSTMVASGFQDSFIKLWSIDGSPLRSLLKNDPYNQQNNDGVAVKGSRRLVGHSGAVYGVDFSPDNRYLISCSEDKTVRLW
SLDTYTCLVSYKGHSSSVWDVKFSPMGHYFATASHDQTARLWSCDHIYPLRIFAGHLNDVDCVEFHPNSTYLFTGSSDKT
ARMWDIARGECVRVFMGHSGAINCLAVSPDGRWLASAGEDSVVCLWDISTGRRIKAMRGHGRSSIYSLAFSREGTVLVST
GADNSVRVWDVKKNTNSPSAQPEPINDVTAQGIQKKTEDLRRRKEIVATNDHMSVYFTKKTPVYTVHFTRRNLCLAGGVF
GG</string>
                    <external_references type="UNIPROTKB">C4R4L4</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="10">
                <name>Subunit (60 kDa) of TFIID and SAGA complexes</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.054888444999999994</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSKNSQRSSIPTSHTLWSPSDTVKDAAESLGIFNLNEEAAKNLAMDIEYRIHEILDQASKFMRHGKRRTLHTSDIDRALK
VLNLEPLYGYDVSRPLVFKEALVGAGQNLYYVDDDEVDFEKLINEPLPKVPRFSTFTAHWLAIEGVQPAIPQNPSPNDIK
NILPINRGSMENMFSLINDEVKEDTNEEFTSTGPSVSSNISNQKQGLEVKPLVKHVLSRELQLYFDKIVEVLLNQEETKE
AELLRNSALQSVRADPGLHQLVPYFIQFISETITKNLKNISLLSTMLELIYSLLMNESLFLEPYVHAIIPCILTLLLAKK
IGNVDDELQKQQQLALRELSASLLERVIEDFGSSYSTLKPRITRTLLRAFVSVNNTTPGTQYGALLGLRGLGSEVIRIVV
LGNVINWSSTFLEKLQQEDQVFLIDTLIETLRVLTKEGKLVKDMKTENGIDNERLKQRVGDLIADRIQACDDAQDIYWGI
FFGEV</string>
                    <external_references type="UNIPROTKB">C4QW33</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="11">
                <name>Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.017303576</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MTNEQAAIPRDVRLLHLIFATQNIYSYQDHVPLQLMDFAYRYTTGTLQDATIYSDHAHASGSHISNAGNAGTNAQLTTED
IRLAIAARTNYQFKPVPPKELLLELAAERNKKPLPAVIPTWGIRLPPEKYCLTGKDWVLEDEEEAVSYKKRKT</string>
                    <external_references type="UNIPROTKB">C4QZS5</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="12">
                <name>Transcription-associated protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii (strain GS115 / ATCC 20864)</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.43805534399999996</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MLHVVQLDDFATRLKAAEDYQSKHSVLSEICDSLETFNAAQDYEYFLKSLIPLFIDVLKEVPVSFVANSPENKLRNITLE
ILHRIPANDALQAYSNEIVDTLMDLLKVENELNGILCMKAITTLHKTFKASLQEKVHPFIDIVIEIYSNIPQVVEEQFNG
NQIDSKENVDSTSRPNSPSFSSQSDDSKQLAQAMFSFKTLAESPITMVSLYSSYKELAASSLGNFIPHVMKVLSLEVAKQ
AEARKAAEEKGIILVNVCKEITNRANYGEFIIGQVKAASFLAYLFIRRQAQTFLEPYQQAIPDIIIRLLQDCPSELSAAR
KELLHATRHILSTDFRKMFIPKIDLLFDLRVLIGEGFTAYETLRPLAYSTVADFIHNVRDHLTPAQLWKSVSIYCKNLQD
DSLALTVQIMSAKLLLNLIEKIMRSESKTESRQLLMVIIDAYTKRFKMLNSRYNGIMKQHATYEKEKQEKQNQERLLTNK
LDGTTPSPSDDKKVELIDEDQDVKMEDPTPEISDQETIKGDNDASTEPQDSEQQLADFMSLQEYLPIQVSVPPEIDLLKD
SRYLFKTLMTFLKTIMIGLKNSNPPSSQNHFNAQNWNETARGFSNEDINILKSLFRECILALRFFSTSKTSLPASSMKQS
FDITGPNLPITSTKEEKDLMEIFATMFIHIDPASFNEIVREELPFMYKQMLDFASLLHIPQFFLASVITSSSFSGILITF
LKSKLVDLGEVNIIKSNILIRLFKLCFMSVSLFPAANESVILPHLNELILKSLKLSTTAKEPLVYFYLIRTLFRSIGGGR
FENLYKEIMPLLQVLLESLSKLIHEARRPQERDIYVELCLTVPVRLSVLVPHLSYLMKPLVYALNGSQESVSQGLRTLEL
CVDNLTAEYFDPIIEPVIDDVMEALSKHLKPLPYYHQHSHTTLRILGKLGGRNRTFIKPVDNLKTDSELFQNVEAMFKIH
GLPNEVPLSITPGLSAAFSLLTDPRPRIHYRINSFKYISGIFQLFLGATQLPDDYANRLKESMDIILEDTIAPDEPLNKL
HHFPVKDIAKYDSQMELLVKLLESIFYAVSLQEVREESKALIRGTCNHFILLYFNKMVIDKRKFVRKFSVDNHEGNLFLN
ENCIFDAIIYALSSDNSAVRSMGLESVQLIYDSCVELFGNIDCALKFAPLNVMCSKFIHCCFEEPYHKKLAGCIGLEMML
NSLDIPMKYFNARQLEIIRALFYVLRDTAPELPCEVTNTAKRLILNSLKEWNKELTRNDVFSSVFQNLVSSLIVDLPNAN
EIVRATAQEALRTLSETTQVPIATMISPCKHILLAPIFGKPLRALPFQMQIGNIDAITFCMGLENSFLEYNEELNRLVQE
ALALVDAEDESLVSAHRISEHKTSEQLVRLRVVCIQLLSLAITKPEFAAAQQRSNIRVKILVVFFKSLCGRSIEIIRAAH
GGLKAVIDLKMKLPKELLQNGLRPMLMNLSDHKKLTVASLEALSGLLKLFISYFKVGIGSKLLDHLLAWAQPRTLQQLGS
QDLENNSTVQIIVAILDVFHLLPPTAHKFMNDLMNALLYLENNLHRCQYSPFREPLAKFLDRFPDESFEYFFNEFSKREI
TTRFVYFVGLDSCSSLRAKVLESLPRVRGLLHQEGSAEEKCVRFSNLVDLCESLAASDKEWIKDKEELLGELLDAGSVCL
TLKRSSNVVSPLYFQVDQGFETLQLLYIEYFKSQPLGHEKVFNFIDKISKEGLPFVLEFDDFIFNEVVKCQDIPTVQQTL
DTIIRMTPQVSSLDARVYLYKRIFLPICIYESEMHGDLSRLSQTENNELPAWLKSFDSDVWKATGPLVDDYTSTLEDRYR
LELMQLTALLIKGAPTALTDMRKDIIKFSWNYIKLDDNTSKQAAYVVTAYFISRFDTPSELTTRIFVALLRCHQIDTRYL
VKQALELLAPVLSERTNSELDWLKWPRRVLSEDGFNITQVANIYQLIVKFPDLFYPARDHFIPNIITAMGKLTVMSNTSL
ENQQLAIDLAELILKWETKLPKSEKLGSAEETEKEKSVSEDKMDIDVKEETKEDIAERPKAEDQIGGDDSDSSNILTSED
YEVSFAQREACVTFLIRYICISTQRPSENELGKRALNILYELLGPKYWSEVTVKLQFFERFLMSSDLNQPSLLGYCLNAL
EVLAVALKWKPTTWIIENVSYLQKLLEKCLRSDNQDIQEILQKVLGIILEAINKETQGSEEDEPEEVTNFISLIVNIIGE
DLSNMTSVAAGVSLCWTLSLYRPNALDSLLPSIMRTFNKLCRDHIAISLQGNQPQSGDFANIEFEAKVTTNLLEKILNLC
AARISSLDDQRRVFLSLLAQLIDRSVDKDMLLKVINIVTEWIFKTDFYPTTKEKAGILGKMMIFDLRGEPELSKKFNQVI
VDIFESKELAHTELTARMETAFLFGTRLSDVSIRKKLMSILSDSLELDIDKRLFYIIKDQNWEYLSDYPWLNQALQLLYG
SFHLDSPIRLSPEENTLSPLQSITEGLAREKSPVEKAPQNIIDFVAKHNEFLDSVRSLTAGDILNPLIDISYQSAETIHN
AWVVVFPVAYSAIESRYELEFTRALVKLLFKDYHIRQQDARPNVIKSLLDGVGKCPGLHLPPHLVKYLGSNYNAWYGAIK
LLEELSEGQGIDNQKISDANQDALLEVYMSLQEDDMFYGTWRRRAKYFETNAALSYEQIGIWDKALQLYEAAQIKARSGV
FPFGESEYSLWEDHWIYCAEKLQHWEILTELAKHEGFTDLLLECGWRGADWIADREPLEQSVKTVMDIPTPRRQIFQTFL
ALQGFSQQKDTLQDVSRLCDEGIQLTLRKWNALPQRVTRAHIGLLHTFQQYVELMEASQVYSSLVTTNAQNLDVKSQELK
RVLQAWRERLPNVWDDINIWNDLVTWRQHVFGVINRVYMPFVPVLQQSNGTNNGNSYAYRGYHEMAWVINRFAHVARKHE
MPEVCINQLTKIYTLPNIEIQEAFLKLREQAKCHYQNSSELNTGLDVISNTNLVYFATQQKAEFFTLKGMFLAKLNAKDE
ANQAFATAVQIDLNLPKAWAEWGFFNDRRFKENPEEIFHAKNAISCYLQAAGLYKDGKTRKLLCRILWLISLDDAAGSLA
KTFEDHHGESPVWYWITFVPQLLTSLSHKEAKIVRHILIQIAKSYPQSLHFQLRTTKEDYQAIQRQAMAVNRAEEQSSNK
QDTADSVLKNTNTPQPQTRTETSGTTAESDKKPSIPPKEEQGSPQPSRPATTQASPQAQSQENGESSQKHPPEIPTTDSR
QPWQDVEEIMGILKTAYPLLALSLESLVDQLNQRFKCNADEDAYRLVIVLYNDGVQQMNRVANPREEVKLPAATEASISR
FADSVLPKNIREVFEQDIIACNPNLETYISKLRKWRDCLEEKLDRSYGKADLERVSLHLSLFHHQKFEDIEIPGQYLLHK
DNNNHFIKIERFLPTLDLVRGSNGCYKRMTIRGNDGSLHPFAVQFPAARHCRREERIFQLFRIFDDALSRKVQSRRRNIS
LTLPIAVPLSPHIRILNDDKRYTTLMGIYEEFCRRKGQSRDEPFAYTIQKLRAAFDPRLPKPDIVSVRAEVLASIQSTLV
PSTLLKDYYTEKFSNYENYWLFRKQFTAQYASFIFMTYIMCINSRQPQKIHINEGSGNIWTSEMLPTKVATGKTHSTAYN
NSTLDPAVKAGAPIFYNTESVPFRLTPNIQKFIGEAGLEGILSVYILVIANSLSDSEFDMEQYLSLFVRDEVISWFAQQH
RASAQTNQLREIVRVNVELLTKRVLQLNHIPNSQNVATQFVLNLISQAVNPRNLAYTDSAWMAYL</string>
                    <external_references type="UNIPROTKB">C4QYV4</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="13">
                <name>Transcriptional adapter 3 (Ada3)</name>
                <natural_source database="NCBI">
                    <organism ncbi="644223">Komagataella phaffii GS115</organism>
                    <strain>GS115 / ATCC 20864</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.006485986</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)</string>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.4</concentration>
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <grid>
                        <model>Quantifoil R2/2</model>
                        <material>COPPER/RHODIUM</material>
                        <mesh>300</mesh>
                        <pretreatment>
                            <type>PLASMA CLEANING</type>
                            <time units="s">90</time>
                            <atmosphere>OTHER</atmosphere>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">95</chamber_humidity>
                        <chamber_temperature units="K">283</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">70.0</c2_aperture_diameter>
                    <nominal_cs units="mm">0.01</nominal_cs>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <calibrated_defocus_min units="µm">0.0008</calibrated_defocus_min>
                    <nominal_defocus_max units="µm">3.5</nominal_defocus_max>
                    <calibrated_defocus_max units="µm">0.0045000000000000005</calibrated_defocus_max>
                    <nominal_magnification>105000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <zemlin_tableau/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Quantum LS</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 QUANTUM (4k x 4k)</film_or_detector_model>
                            <detector_mode>SUPER-RESOLUTION</detector_mode>
                            <average_exposure_time units="s">8.0</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">52.8</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>1068534</number_selected>
                </particle_selection>
                <startup_model type_of_model="EMDB MAP"/>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C1</point_group>
                    </applied_symmetry>
                    <algorithm>FOURIER SPACE</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">3.8</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>354104</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="536871">
        <file>emd_10438.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>512</col>
            <row>512</row>
            <sec>512</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>512</x>
            <y>512</y>
            <z>512</z>
        </spacing>
        <cell>
            <a units="Å">558.08</a>
            <b units="Å">558.08</b>
            <c units="Å">558.08</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-1.5468898</minimum>
            <maximum>3.1253965</maximum>
            <average>-0.00081884454</average>
            <std>0.058872446</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.09</x>
            <y units="Å">1.09</y>
            <z units="Å">1.09</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.7</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-10438::::</label>
        <annotation_details>SAGA-TBP structure refined in CryoSparc</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>OTHER</refinement_protocol>
            </modelling>
        </modelling_list>
        <segmentation_list>
            <segmentation>
                <file>emd_10438_msk_1.map</file>
            </segmentation>
        </segmentation_list>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="536871">
                <file>emd_10438_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>512</col>
                    <row>512</row>
                    <sec>512</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>512</x>
                    <y>512</y>
                    <z>512</z>
                </spacing>
                <cell>
                    <a units="Å">558.08</a>
                    <b units="Å">558.08</b>
                    <c units="Å">558.08</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.67704463</minimum>
                    <maximum>1.9909145</maximum>
                    <average>0.002472744</average>
                    <std>0.08557669</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.09</x>
                    <y units="Å">1.09</y>
                    <z units="Å">1.09</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-10438::::</label>
                <annotation_details>Half B map obtained with CryoSparc</annotation_details>
            </half_map>
            <half_map format="CCP4" size_kbytes="536871">
                <file>emd_10438_half_map_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>512</col>
                    <row>512</row>
                    <sec>512</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>512</x>
                    <y>512</y>
                    <z>512</z>
                </spacing>
                <cell>
                    <a units="Å">558.08</a>
                    <b units="Å">558.08</b>
                    <c units="Å">558.08</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.8198041</minimum>
                    <maximum>2.0049012</maximum>
                    <average>0.0024595018</average>
                    <std>0.08576088</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.09</x>
                    <y units="Å">1.09</y>
                    <z units="Å">1.09</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-10438::::</label>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
