2020-07-22 REL PDBe PDBe PDBe 2019-10-24 2019-11-20 2019-11-20 2020-07-22 Wellcome Trust 202754/Z/16/Z United Kingdom European Molecular Biology Organization ALTF-128-2016 FtsK motor domain bound to dsDNA, AMPPNP state Jean NL Lowe J Jean NL Rutherford TJ Lowe J FtsK in motion reveals its mechanism for double-stranded DNA translocation. Proc.Natl.Acad.Sci.USA US 117 14202 14208 2020 32513722 doi:10.1073/pnas.2001324117 1091-6490 0040 PNASA6 Jean NL Rutherford TJ Lowe J FtsK in motion reveals its mechanism for double-stranded DNA translocation Biorxiv US doi:10.1101/828319 EMD-10399 other EM volume EMD-10405 associated EM volume EMD-10400 other EM volume EMD-10402 other EM volume EMD-10403 other EM volume EMD-10404 other EM volume FtsK motor domain bound to dsDNA, AMPPNP-state FtsK motor domain bound to dsDNA, AMPPNP-state 0 1 2 FtsK motor domain, AMPPNP-state 1 1 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Escherichia coli BL21(DE3) DNA 1 2 synthetic construct synthetic construct DNA translocase FtsK Pseudomonas aeruginosa PAO1 Escherichia coli BL21(DE3) LEVO MVPDRREQSKAKERLLEREEALAKHMSEREKRPPPKIDPPPSPKAPEPSKRVLKEKQAPLFVDTAVEGTLPPLSLLDPAEVKQKSYSPESLEAMSRLLEIKLKEFGVEVSVDSVHPGPVITRFEIQPAAGVKVSRISNLAKDLARSLAVISVRVVEVIPGKTTVGIEIPNEDRQMVRFSEVLSSPEYDEHKSTVPLALGHDIGGRPIITDLAKMPHLLVAGTTGSGKSVGVNAMLLSILFKSTPSEARLIMIDPKMLELSIYEGIPHLLCPVVTDMKEAANALRWSVAEMERRYRLMAAMGVRNLAGFNRKVKDAEEAGTPLTDPLFRRESPDDEPPQLSTLPTIVVVVDEFADMMMIVGKKVEELIARIAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTILDQGGAEQLLGHGDMLYLPPGTGLPIRVHGAFVSDDEVHRVVEAWKLRGAPDYIEDILAGVDEGGKLHHHHHH dsDNA substrate unidentified ATATATATATATATATATAT DNA singleParticle particle 7.5 25.0 Tris 2.0 AMPPNP 4.0 MgCl2 Quantifoil R1.2/1.3 GOLD 300 CARBON HOLEY ARRAY GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV
0.7 m/mL FtsK 1.5 uM 45 bp DNA
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 830 48.6 1 CTFFIND 4.1 2IUU
40 A low-pass filtered map generated from PDB 2IUU
 C1 4.8 FSC 0.143 CUT-OFF RELION 3.0 28958 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 emd_10405.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 264.0 264.0 264.0 90.0 90.0 90.0 X Y Z -0.03399552 0.07121996 0.00037384237 0.0030284023 1.1 1.1 1.1 0.0209 AUTHOR