Cryo-electron tomogram containing EIAV Gag-deltaMA spheres and tubes assembled at pH6

2020-11-18 REL PDBe PDBe PDBe 2019-10-17 2020-01-08 2020-01-15 2020-11-18 German Research Foundation BR 3635/2-1 Germany Austrian Science Fund P31445 Austria National Institutes of Health/National Institute of General Medical Sciences R01-GM107013 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases R01-AI147890 United States National Institutes of Health/National Institute of General Medical Sciences P30-GM110758 United States National Science Foundation (United States) 1659534 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases AI142263 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases P50AI150481 United States Medical Research Council (United Kingdom) MC_UP_1201/16 United Kingdom European Research Council ERC-2014-CoG 648432 - MEMBRANEFUSION United Kingdom Cryo-electron tomogram containing EIAV Gag-deltaMA spheres and tubes assembled at pH6 Dick RA Xu C Morado DR Kravchuk V Ricana CL Lyddon TD Broad AM Feathers JR Johnson MC Vogt VM Perilla JR Briggs JAG Schur FKM Dick RA Xu C Morado DR Kravchuk V Ricana CL Lyddon TD Broad AM Feathers JR Johnson MC Vogt VM Perilla JR Briggs JAG Schur FKM Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly. Plos Pathog. US 16 e1008277 e1008277 2020 31986188 doi:10.1371/journal.ppat.1008277 1553-7374 EMD-10383 other EM volume

This entry contains the structure for EIAV Gag-deltaMA tubes assembled at pH6

EMD-10384 other EM volume

This entry contains the structure for EIAV Gag-deltaMA spheres assembled at pH6

EMD-10386 associated EM volume Equine infectious anemia virus Equine infectious anemia virus 0 1

Gag construct was expressed in E.coli and purified using the SUMO-tag system. Assembly was performed at pH6.

Equine infectious anemia virus Equus caballus Escherichia coli BL21 Capsid 350.0 VIRUS-LIKE PARTICLE OTHER false true tomography particle 6 50.0 MES 2-(N-morpholino)ethanesulfonic acid 100.0 NaCl Sodium chloride 2.0 TCEP tris(2-carboxyethyl)phosphine C-flat-2/2 COPPER 300 CARBON HOLEY GLOW DISCHARGE AIR

20mA

ETHANE 90 15 FEI VITROBOT MARK II

1-2 seconds blot time, offset -3mm.

Virus-like-particles (tubular) of EIAV Gag deltaMAdeltap9 (referred to as Gag deltaMA) assembled at pH6.

BBI 10 NO SECTIONING FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 -1.5 -3.5 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20

nanoprobe

GATAN K2 QUANTUM (4k x 4k) SUPER-RESOLUTION 3708 3838 1 1.4 3.4

Data was acquired using a dose-symmetric tilt acquisition scheme, as described in Hagen et al, 2017, J. Struct. Biol, 197(2):191-8

Tilt series were low-pass filtered according to their cumulative dose using exposure filters that were calculated using an exposure-dependent amplitude attenuation function and critical exposure constants (as published in Grant & Grigorieff, Elife, 2015). Tilt series were aligned and reconstructed in IMOD.

BACK PROJECTION IMOD 41 CTFFIND 4 NOVACTF

CTF-correction was performed using NOVACTF

emd_10386.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 464 464 165 0 0 50 464 464 165 3864.1921 3864.1921 1374.12 90.0 90.0 90.0 X Y Z -3434.792 3399.958 103.01864 83.679924 8.328 8.328 8.328 AUTHOR ::::EMDATABANK.org::::EMD-10386:::: Cryo-electron tomogram containing EIAV Gag-deltaMA spheres and tubes assembled at pH6