2020-06-03 REL PDBe PDBe PDBe 2019-10-15 2019-11-13 2019-11-13 2020-06-03 Medical Research Council (United Kingdom) MC_UU_00012/6 United Kingdom Swiss National Science Foundation 31003A_179517 Switzerland European Research Council ERC-2012-SyG_318987-ToPAG Germany German Research Foundation EXC 2067/1- 390729940 Germany European Research Council ERC AdG TENDO - 834394 Switzerland Spanish Ministry of Economy and Competitiveness SEV-2015-0522 Spain German Research Foundation SFB1190/P22 Germany Spanish Ministry of Economy and Competitiveness FIS2017-89560-R Spain Spanish Ministry of Economy and Competitiveness FIS2015-63550-R Spain Spanish Ministry of Economy and Competitiveness RYC-2017-22227 Spain Spanish Ministry of Economy and Competitiveness BFU2015-73288-JIN Spain Cryo-electron tomogram of cER-PM MCS in a heat-shocked WT S. cerevisiae cell Collado J Fernandez-Busnadiego R Collado J Fernandez-Busnadiego R Kalemanov M Campelo F Bourgoint C Thomas F Loewith R Martinez-Sanchez A Stefan CJ Baumeister W Tricalbin-Mediated Contact Sites Control ER Curvature to Maintain Plasma Membrane Integrity Dev.Cell US 2019 doi:10.1016/j.devcel.2019.10.018 1534-5807 EMD-10378 associated EM volume ER-PM membrane contact site ER-PM membrane contact site 0
Yeast cells vitrified after heat shock
Saccharomyces cerevisiae SEY6210.1 tomography cell 5.5
YPD medium
 Quantifoil R2/1 COPPER 200 CARBON HOLEY PLASMA CLEANING AIR 101.325 ETHANE 21 300 FEI VITROBOT MARK IV
OTHER OTHER 30 0.03 7200 90 4000 200
The value given for _emd_sectioning_focused_ion_beam.instrument is FEI Quanta FIB. This is not in a list of allowed values set(['DB235', 'OTHER']) so OTHER is written into the XML file.
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 42000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 80.0 100.0 GIF Quantum LS 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 3838 3710 1 1.8 1.7 1 BACK PROJECTION IMOD 4.9
Tomogram was binned twice.
55 emd_10378.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 928 928 189 0 94 0 928 928 189 12695.04 12695.04 2585.52 90.0 90.0 90.0 X Y Z -154 83 2.817814 11.099517 13.68 13.68 13.68 AUTHOR