2020-05-13 REL PDBe PDBe PDBe 2019-10-13 2020-04-22 2020-04-22 2020-05-13 Medical Research Council (United Kingdom) MR/M00936X/1 United Kingdom Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIV(5A)-a) Marechal A Hartley A Pinotsis N Hartley AM Meunier B Pinotsis N Marechal A Rcf2 revealed in cryo-EM structures of hypoxic isoforms of mature mitochondrial III-IV supercomplexes. Proc.Natl.Acad.Sci.USA US 117 9329 9337 2020 32291341 doi:10.1073/pnas.1920612117 1091-6490 0040 PNASA6 EMD-0269 other EM volume EMD-10375 associated EM volume Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIV(5A)-a) Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIV(5A)-a) 0 1 2 3 4 5 6 7 8 9 10 11 12 13
13-subunit cytochrome c oxidase with isoform Cox5A
 Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIV(5A)-a) 1 1 2 3 4 5 6 7 8 9 10 12 13
13-subunit cytochrome c oxidase with isoform Cox5A
Saccharomyces cerevisiae S288C Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIV(5A)-a) 1 11
13-subunit cytochrome c oxidase with isoform Cox5A
Saccharomyces cerevisiae S288C Saccharomyces cerevisiae S288C CYTOCHROME C OXIDASE SUBUNIT 1; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE I, COX1 Saccharomyces cerevisiae S288C LEVO MVQRWLYSTNAKDIAVLYFMLAIFSGMAGTAMSLIIRLELAAPGSQYLHGNSQLFNVLVV GHAVLMIFFLVMPALIGGFGNYLLPLMIGATDTAFPRINNIAFWVLPMGLVCLVTSTLVE SGAGTGWTVYPPLSSIQAHSGPSVDLAIFALHLTSISSLLGAINFIVTTLNMRTNGMTMH KLPLFVWSIFITAFLLLLSLPVLSAGITMLLLDRNFNTSFFEVSGGGDPILYEHLFWFFG HPEVYILIIPGFGIISHVVSTYSKKPVFGEISMVYAMASIGLLGFLVWSHHMYIVGLDAD TRAYFTSATMIIAIPTGIKIFSWLATIHGGSIRLATPMLYAIAFLFLFTMGGLTGVALAN ASLDVAFHDTYYVVGHFHYVLSMGAIFSLFAGYYYWSPQILGLNYNEKLAQIQFWLIFIG ANVIFFPMHFLGINGMPRRIPDYPDAFAGWNYVASIGSFIATLSLFLFIYILYDQLVNGL NNKVNNKSVIYNKAPDFVESNTIFNLNTVKSSSIEFLLTSPPAVHSFNTPAVQS CYTOCHROME C OXIDASE SUBUNIT 2; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE II, COX2 Saccharomyces cerevisiae S288C LEVO DVPTPYACYFQDSATPNQEGILELHDNIMFYLLVILGLVSWMLYT IVMTYSKNPIAYKYIKHGQTIEVIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAI GYQWYWKYEYSDFINDSGETVEFESYVIPDELLEEGQLRLLDTDTSMVVPVDTHIRFVVT AADVIHDFAIPSLGIKVDATPGRLNQVSALIQREGVFYGACSELCGTGHANMPIKIEAVS LPKFLEWLNEQ CYTOCHROME C OXIDASE SUBUNIT 3; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE III, COX3 Saccharomyces cerevisiae S288C LEVO MTHLERSRHQQHPFHMVMPSPWPIVVSFALLSLALSTALTMHGYIGNMNMVYLALFVLLT SSILWFRDIVAEATYLGDHTMAVRKGINLGFLMFVLSEVLIFAGLFWAYFHSAMSPDVTL GACWPPVGIEAVQPTELPLLNTIILLSSGATVTYSHHALIAGNRNKALSGLLITFWLIVI FVTCQYIEYTNAAFTISDGVYGSVFYAGTGLHFLHMVMLAAMLGVNYWRMRNYHLTAGHH VGYETTIIYTHVLDVIWLFLYVVFYWWGV CYTOCHROME C OXIDASE SUBUNIT 4, MITOCHONDRIAL; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE IV, COX4 Saccharomyces cerevisiae S288C LEVO QQKPVVKTAQNLAEVNGPETLIGPGAKEGTVPTDL DQETGLARLELLGKLEGIDVFDTKPLDSSRKGTMKDPIIIESYDDYRYVGCTGSPAGSHT IMWLKPTVNEVARCWECGSVYKLNPVGVPNDDHHH CYTOCHROME C OXIDASE POLYPEPTIDE 5A, MITOCHONDRIAL; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VA, COX5A Saccharomyces cerevisiae S288C LEVO MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK CYTOCHROME C OXIDASE SUBUNIT 6, MITOCHONDRIAL; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VI, COX6 Saccharomyces cerevisiae S288C LEVO SDAHDEETFEEFTARYEKEF DEAYDLFEVQRVLNNCFSYDLVPAPAVIEKALRAARRVNDLPTAIRVFEALKYKVENEDQ YKAYLDELKDVRQELGVPLKEELFPSSS 1.9.3.1 CYTOCHROME C OXIDASE SUBUNIT 7; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VII, COX7 Saccharomyces cerevisiae S288C LEVO ANKVIQLQKIFQSSTKPLWWRHPRSALYLYPFYAIFAVAVVTPLLYIPNAIRGIKAKKA 1.9.3.1 CYTOCHROME C OXIDASE POLYPEPTIDE VIII, MITOCHONDRIAL; SYNONYM: COX8 Saccharomyces cerevisiae S288C LEVO MLCQQMIRTTAKRSSNIMTRPIIMKRSVHFKDGVYENIPFKVKGRKTPYALSHFGFFAIGFAVPFVACYVQLKKSGAF 1.9.3.1 CYTOCHROME C OXIDASE SUBUNIT 7A; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VIIA, COX9 Saccharomyces cerevisiae S288C LEVO TIAPITGTIKRRVIMDIVLGFSLGGVMASYWWWGFHMDKINKREKFYAELAERKK 1.9.3.1 CYTOCHROME C OXIDASE SUBUNIT 6B; SYNONYM: CYTOCHROME C OXIDASE POLYPEPTIDE VIB, COX12 Saccharomyces cerevisiae S288C LEVO DQENSPLHTVGFDARFPQQNQTKHCWQSYVDYHKCVNMKGEDFAPCKVFWKTYNALCP LDWIEKWDDQREKGIFAGDINSD 1.9.3.1 CYTOCHROME C OXIDASE SUBUNIT 6A, MITOCHONDRIAL; CYTOCHROME C OXIDASE POLYPEPTIDE VIA, COX13 Saccharomyces cerevisiae S288C Saccharomyces cerevisiae S288C LEVO ASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVAL PAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPV VNRHIEHDDG ARGSHHHHHH 1.9.3.1 COX26; SYNONYM: Uncharacterized protein YDR119W-A Saccharomyces cerevisiae S288C LEVO MFFSQVLRSSARAAPIKRYTGGRIGESWVITEGRRLIPEIFQWSAVLSVCLGWPGAVYFF SKARKA 1.9.3.1 RCF2; SYNONYM: Respiratory supercomplex factor 2 Saccharomyces cerevisiae S288C LEVO MKILTQDEIEAHRSHTLKGGIEGALAGFAISAIIFKVLPRRYPKFKPSTLTWSIKTALWI TPPTVLTAICAEEASNNFDATMYGSGSSSEDALDEHRRWKSLSTKDKFVEGLSNNKYKII TGAWAASLYGSWVIVNKDPIMTKAQKIVQARMYAQFITVGLLLASVGLSMYENKLHPNKQ KVNEMRRWENALRVAEEEERLEKEGRRTGYVSNEERINSKIFKS 1.9.3.1 singleParticle particle 7.2 ETHANE FEI VITROBOT MARK IV
3 uL of sample applied to negatively glow discharged grid, blot force -10; blotting time 8.5 sec..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 2740 52.64 1 CTFFIND 4 within cryosparc 2 6T15 C1 3.09 FSC 0.143 CUT-OFF cryoSPARC 2 44194 NOT APPLICABLE NOT APPLICABLE emd_10375.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 536.576 536.576 536.576 90.0 90.0 90.0 X Y Z -2.1611605 4.423754 0.0023443839 0.11214336 1.048 1.048 1.048 0.46 AUTHOR The reprocessed CIVa map after focused refinement with a mask including rcf2