2020-11-25 REL PDBe PDBe PDBe 2019-09-26 2019-10-30 2019-11-06 2020-11-25 Spanish Ministry of Science, Innovation, and Universities PGC2018-098701-B-I00 Spain Spanish Ministry of Economy and Competitiveness SEV-2016-0644 Spain Spanish Ministry of Science, Innovation, and Universities RTI2018-095700-B-I00 Spain Structure of African Swine fever virus Abrescia NG Andres G Charro D Matamoros T Dillard R Andres G Charro D Matamoros T Dillard RS Abrescia NGA The cryo-EM structure of African swine fever virus unravels a unique architecture comprising two icosahedral protein capsids and two lipoprotein membranes. J.Biol.Chem. US 2019 31649031 doi:10.1074/jbc.AC119.011196 1083-351X 0071 JBCHA3 EMD-10325 other EM volume EMD-10346 associated EM volume African swine fever virus BA71V African swine fever virus BA71V 0 1 African swine fever virus BA71V pigs, warthogs, bushpigs Outer capsid (MCP p72) 2400.0 277 VIRION STRAIN true false singleParticle particle 1.1 7.4 PBS Quantifoil R2/2 COPPER 200 GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK III
FEI TITAN KRIOS FLOOD BEAM DARK FIELD FIELD EMISSION GUN 300
Two datasets were acquired.
FEI FALCON III (4k x 4k) 47.7
Titan Krios equipped with a Cs-corrector and a Falcon 3EC direct electron detector
 GATAN K2 SUMMIT (4k x 4k) 47.5
Titan Krios equipped with a Gatan K2 Summit direct electron detector
 2 1267
583 from Falcon 3EC dataset plus 684 from K2 dataset
 CTFFIND 2 I FOURIER SPACE 23.1 FSC 0.143 CUT-OFF RELION 1110 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION 2 RELION
From the first data collection 583 ASFV particles were visually selected and extracted in RELION, and 3D classified in two classes (as a reference an initial symmetrized model was generated in RELION). The largest class with 450 particles was refined and a preliminary icosahedral model of the virion was produced; this model served as reference for the 3D classification of the second dataset (684 starting particles) which led to a largest class with 650 particles. Then both set of particles were interpolated to the same pixel size and merged for 3D reconstruction.
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