2020-05-13 REL PDBe PDBe PDBe 2019-09-24 2019-10-09 2020-04-22 2020-05-13 Medical Research Council (United Kingdom) MR/M00936X/1 United Kingdom CIV1 of the III2-IV5B2 respiratory supercomplex from S. cerevisiae after focused refinement Marechal A Hartley A Pinotsis N Hartley AM Meunier B Pinotsis N Marechal A Rcf2 revealed in cryo-EM structures of hypoxic isoforms of mature mitochondrial III-IV supercomplexes. Proc.Natl.Acad.Sci.USA US 117 9329 9337 2020 32291341 doi:10.1073/pnas.1920612117 1091-6490 0040 PNASA6 EMD-10334 other EM volume
Contains the second complex IV in the CIII2-CIV(5B)2 supercomplex
 EMD-10335 associated EM volume CIV of the III2-IV(5B)2 respiratory supercomplex from S. cerevisiae after focused refinement (CIV(5B)-a) CIV of the III2-IV(5B)2 respiratory supercomplex from S. cerevisiae after focused refinement (CIV(5B)-a) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Saccharomyces cerevisiae S288C Cox1 Cytochrome c oxidase subunit 1 Saccharomyces cerevisiae S288C LEVO MVQRWLYSTNAKDIAVLYFMLAIFSGMAGTAMSLIIRLELAAPGSQYLHGNSQLFNVLVV GHAVLMIFFLVMPALIGGFGNYLLPLMIGATDTAFPRINNIAFWVLPMGLVCLVTSTLVE SGAGTGWTVYPPLSSIQAHSGPSVDLAIFALHLTSISSLLGAINFIVTTLNMRTNGMTMH KLPLFVWSIFITAFLLLLSLPVLSAGITMLLLDRNFNTSFFEVSGGGDPILYEHLFWFFG HPEVYILIIPGFGIISHVVSTYSKKPVFGEISMVYAMASIGLLGFLVWSHHMYIVGLDAD TRAYFTSATMIIAIPTGIKIFSWLATIHGGSIRLATPMLYAIAFLFLFTMGGLTGVALAN ASLDVAFHDTYYVVGHFHYVLSMGAIFSLFAGYYYWSPQILGLNYNEKLAQIQFWLIFIG ANVIFFPMHFLGINGMPRRIPDYPDAFAGWNYVASIGSFIATLSLFLFIYILYDQLVNGL NNKVNNKSVIYNKAPDFVESNTIFNLNTVKSSSIEFLLTSPPAVHSFNTPAVQS Cox2 Cytochrome c oxidase subunit 2 Saccharomyces cerevisiae S288C LEVO MLDLLRLQLTTFIMNDVPTPYACYFQDSATPNQEGILELHDNIMFYLLVILGLVSWMLYT IVMTYSKNPIAYKYIKHGQTIEVIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAI GYQWYWKYEYSDFINDSGETVEFESYVIPDELLEEGQLRLLDTDTSMVVPVDTHIRFVVT AADVIHDFAIPSLGIKVDATPGRLNQVSALIQREGVFYGACSELCGTGHANMPIKIEAVS LPKFLEWLNEQ Cox3 Cytochrome c oxidase subunit 3 Saccharomyces cerevisiae S288C LEVO MTHLERSRHQQHPFHMVMPSPWPIVVSFALLSLALSTALTMHGYIGNMNMVYLALFVLLT SSILWFRDIVAEATYLGDHTMAVRKGINLGFLMFVLSEVLIFAGLFWAYFHSAMSPDVTL GACWPPVGIEAVQPTELPLLNTIILLSSGATVTYSHHALIAGNRNKALSGLLITFWLIVI FVTCQYIEYTNAAFTISDGVYGSVFYAGTGLHFLHMVMLAAMLGVNYWRMRNYHLTAGHH VGYETTIIYTHVLDVIWLFLYVVFYWWGV Cox4 Cytochrome c oxidase subunit 4 Saccharomyces cerevisiae S288C LEVO MLSLRQSIRFFKPATRTLCSSRYLLQQKPVVKTAQNLAEVNGPETLIGPGAKEGTVPTDL DQETGLARLELLGKLEGIDVFDTKPLDSSRKGTMKDPIIIESYDDYRYVGCTGSPAGSHT IMWLKPTVNEVARCWECGSVYKLNPVGVPNDDHHH Cox5B Cytochrome c oxidase subunit 5B Saccharomyces cerevisiae S288C LEVO MLRTSLTKGARLTGTRFVQTKALSKATLTDLPERWENMPNLEQKEIADNLTERQKLPWKT LNNEEIKAAWYISYGEWGPRRPVHGKGDVAFITKGVFLGLGISFGLFGLVRLLANPETPK TMNREWQLKSDEYLKSKNANPWGGYSQVQSK Cox6 Cytochrome c oxidase subunit 6 Saccharomyces cerevisiae S288C LEVO MLSRAIFRNPVINRTLLRARPGAYHATRLTKNTFIQSRKYSDAHDEETFEEFTARYEKEF DEAYDLFEVQRVLNNCFSYDLVPAPAVIEKALRAARRVNDLPTAIRVFEALKYKVENEDQ YKAYLDELKDVRQELGVPLKEELFPSSS Cox7 Cytochrome c oxidase subunit 7 Saccharomyces cerevisiae S288C LEVO MANKVIQLQKIFQSSTKPLWWRHPRSALYLYPFYAIFAVAVVTPLLYIPNAIRGIKAKKA Cox8 Cytochrome c oxidase polypeptide VIII Saccharomyces cerevisiae S288C LEVO MLCQQMIRTTAKRSSNIMTRPIIMKRSVHFKDGVYENIPFKVKGRKTPYALSHFGFFAIG FAVPFVACYVQLKKSGAF Cox9 Cytochrome c oxidase subunit 7A Saccharomyces cerevisiae S288C LEVO MTIAPITGTIKRRVIMDIVLGFSLGGVMASYWWWGFHMDKINKREKFYAELAERKKQEN Cox12 Cytochrome c oxidase subunit 6B Saccharomyces cerevisiae S288C LEVO MADQENSPLHTVGFDARFPQQNQTKHCWQSYVDYHKCVNMKGEDFAPCKVFWKTYNALCP LDWIEKWDDQREKGIFAGDINSD Cox13 Cytochrome c oxidase subunit 6A Saccharomyces cerevisiae S288C LEVO MFRQCAKRYASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHAKDTSNMWVKISVWVAL PAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIRSKPFFWGDGDKTLFWNPV VNRHIEHDDG ARGSHHHHHH Cox26, Uncharacterized protein YDR119W-A Saccharomyces cerevisiae S288C LEVO MFFSQVLRSSARAAPIKRYTGGRIGESWVITEGRRLIPEIFQWSAVLSVCLGWPGAVYFF SKARKA RCF2, Respiratory supercomplex factor 2, mitochondrial Saccharomyces cerevisiae S288C LEVO MKILTQDEIEAHRSHTLKGGIEGALAGFAISAIIFKVLPRRYPKFKPSTLTWSIKTALWI TPPTVLTAICAEEASNNFDATMYGSGSSSEDALDEHRRWKSLSTKDKFVEGLSNNKYKII TGAWAASLYGSWVIVNKDPIMTKAQKIVQARMYAQFITVGLLLASVGLSMYENKLHPNKQ KVNEMRRWENALRVAEEEERLEKEGRRTGYVSNEERINSKIFKS singleParticle particle 7.2 ETHANE FEI VITROBOT MARK IV
3 microliter of sample applied to negatively glow discharged grid, blot force -10; blotting time 8.5 sec..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 56.4 1 cryoSPARC 2 C1 2.8 FSC 0.143 CUT-OFF cryoSPARC 2 65999 MAXIMUM LIKELIHOOD cryoSPARC 2 MAXIMUM LIKELIHOOD cryoSPARC 2 cryoSPARC 2 emd_10335.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 448 448 448 0 0 0 448 448 448 486.08002 486.08002 486.08002 90.0 90.0 90.0 X Y Z -1.9322762 3.338631 -0.001714312 0.069522366 1.085 1.085 1.085 0.32 AUTHOR CIV(5B)-a of III2-IV(5B)2 respiratory supercomplex from S. cerevisiae after focused refinement