2021-04-14 REL PDBe PDBe PDBe 2019-09-12 2019-09-25 2019-11-13 2021-04-14 German Research Foundation 251981924TRR83 Germany German Research Foundation 347368302 FOR2682 Germany European Research Council CROWDED-PRO-LIPIDS Finland Academy of Finland Finland Human insulin receptor ectodomain bound by several insulins in an intermediate state Gutmann T Schaefer IB Poojari C Brankatschk B Vattulainen I Strauss M Coskun U Gutmann T Schafer IB Poojari C Brankatschk B Vattulainen I Strauss M Coskun U Cryo-EM structure of the complete and ligand-saturated insulin receptor ectodomain. J.Cell Biol. US 219 2020 31727777 doi:10.1083/jcb.201907210 1540-8140 2019 JCLBA3 Gutmann T Schafer I Poojari C Brankatschk B Vattulainen I Strauss M Coskun U Cryo-EM structure of the complete and ligand-saturated insulin receptor ectodomain Biorxiv US 679233 2019 doi:10.1101/679233 EMD-10273 other EM volume EMD-10311 associated EM volume human insulin receptor ectodomain bound by several insulins in an intermediate state human insulin receptor ectodomain bound by several insulins in an intermediate state 0 1 Homo sapiens Homo sapiens 0.375 human insulin receptor ectodomain Homo sapiens Homo sapiens LEVO HLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLE SLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSV EDNYIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHS ECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIH NNKCIPECPSGYTMNSSNLLCTPCLGPCPKVCHLLEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNL AAELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLT ITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSYIRTSFDKILLRWE PYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLRSNDPKSQNHPGWLMRGLKPWT QYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLV FWERQAEDSELFELDYCLKGLKLPSRTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESS FRKTFEDYLHNVVFVPRPS HRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFEN NVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNG SWTEPTYFYVTDYLDVPSNIAK singleParticle particle 7.5 ETHANE-PROPANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 1 51 10.2 55.0 1 Gctf 1 C1 5.0 FSC 0.143 CUT-OFF RELION 3.0 50079 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 3.0 RELION 3.0 emd_10311.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 -144 -144 -144 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.6419248 0.9117107 0.0018253716 0.030943278 1.06 1.06 1.06 0.013 AUTHOR manually filtered, sharpened and masked map. In manuscript labeled as "intermediate state". On different origin as the other maps. 6CEB FLEXIBLE FIT REAL emd_10311_msk_1.map emd_10311_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.010905224 0.045333058 0.00022205902 0.0017072123 1.06 1.06 1.06 AUTHOR automatically filtered, sharpened and masked map. In manuscript labeled as "intermediate state". emd_10311_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.009077852 0.039268397 5.8748054e-05 0.0018262244 1.06 1.06 1.06 AUTHOR half-map 2 used as input for postprocessing to generate the main map (post Bayesian polishing and 3D refinement) emd_10311_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.0075069554 0.040145174 5.8981015e-05 0.0018243248 1.06 1.06 1.06 AUTHOR half-map 1 used as input for postprocessing to generate the main map (post Bayesian polishing and 3D refinement)