2020-11-25 REL PDBe PDBe PDBe 2019-09-05 2019-11-06 2019-11-06 2020-11-25 Medical Research Council (United Kingdom) MC_U105192715 United Kingdom Structure of the Fanconi Anaemia core complex (focussed map for top region) Shakeel S Rajendra E Alcon P He S Scheres SHW Passmore LA Shakeel S Rajendra E Alcon P O'Reilly F Chorev DS Maslen S Degliesposti G Russo CJ He S Hill CH Skehel JM Scheres SHW Patel KJ Rappsilber J Robinson CV Passmore LA Structure of the Fanconi anaemia monoubiquitin ligase complex. Nature UK 575 234 237 2019 31666700 doi:10.1038/s41586-019-1703-4 1476-4687 0006 NATUAS EMD-10290 other EM volume
consensus map
 EMD-10291 associated EM volume Fanconi anaemia core complex Fanconi anaemia core complex 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Gallus gallus Spodoptera frugiperda Sf9 insect cells pACEBac1 0.86 singleParticle particle 0.7 8
50 mM HEPES pH 8.0, ~500 mM NaCl, 1 mM TCEP
 Quantifoil, UltrAuFoil, R1.2/1.3 GOLD 300 HOLEY PLASMA CLEANING ETHANE 100 277.15 FEI VITROBOT MARK IV
Blot time: 3 to 4.5 s Wait time: 0 s Drain time: 0 s Force: -10 N.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 -1.8 -4.0 75000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON III (4k x 4k) COUNTING 4145 40.0 1 Gctf
initial model generated in EMAN2.
 C1 4.5 FSC 0.143 CUT-OFF RELION 3.0
A tau_fudge (T) value of 5 was used during autorefinement.
169000 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 emd_10291.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 -190 -190 -190 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.049035735 0.08699351 0.00005782780 0.0014588226 1.07 1.07 1.07 0.0188 AUTHOR None OTHER emd_10291_msk_1.map emd_10291_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 -190 -190 -190 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.02045223 0.054157123 0.0000588247 0.0010697157 1.07 1.07 1.07 AUTHOR None emd_10291_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.014689129 0.044487473 -0.00012923665 0.001094089 1.07 1.07 1.07 AUTHOR This is the map from auto refinement in Relion with T=5 emd_10291_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.018603051 0.046140853 -0.00012319151 0.0012738439 1.07 1.07 1.07 AUTHOR This is half2 map from auto refinement in Relion with T=5. emd_10291_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.017513696 0.0484259 -0.00012262892 0.0012717273 1.07 1.07 1.07 AUTHOR This is half1 map from auto refinement in Relion with T=5.