2024-10-23 REL PDBe PDBe PDBe 2019-08-29 2019-11-13 2019-11-13 2024-10-23 German Research Foundation 251981924TRR83 Germany German Research Foundation 347368302 FOR2682 Germany European Research Council CROWDED-PRO-LIPIDS Finland Academy of Finland Finland human insulin receptor ectodomain bound by 4 insulin Gutmann T Schaefer IB cell surface receptor, insulin, tyrosine kinase receptor, glucose homeostasis, hormone, diabetes, MEMBRANE PROTEIN Gutmann T Schafer IB Poojari C Brankatschk B Vattulainen I Strauss M Coskun U Cryo-EM structure of the complete and ligand-saturated insulin receptor ectodomain. J.Cell Biol. US 219 2020 31727777 doi:10.1083/jcb.201907210 1540-8140 2019 JCLBA3 Gutmann T Schafer IB Poojari C Brankatschk B Vattulainen I Strauss M Coskun U Cryo-EM structure of the complete and ligand-saturated insulin receptor ectodomain bioRxiv US 2019 doi:10.1101/679233 2692-8205 6sof FULLOVERLAP human insulin receptor ectodomain bound to four insulin human insulin receptor ectodomain bound to four insulin 0 1 2 3 4 0.375 human insulin receptor ectodomain bound to four insulin 1 1 2 Homo sapiens human insulin receptor ectodomain bound to four insulin 1 3 4 Homo sapiens Insulin receptor Homo sapiens 0.082551742 2 Homo sapiens LEVO HLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLT VIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDICP GTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVET CPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGPCPKVCHLLEGEKT IDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSF YALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSYIRT SFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLRSNDPKSQNHPGWLMRGLKPWT QYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVFWERQAEDSE LFELDYCLKGLKLPSRTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRPS P06213 2.7.10.1 Insulin receptor Homo sapiens 0.0185139 2 Homo sapiens LEVO HRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEP KEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNI AK P06213 2.7.10.1 Insulin Homo sapiens 0.0023836979999999996 4 Saccharomyces cerevisiae LEVO GIVEQCCTSICSLYQLENYCN P01308 Insulin Homo sapiens 0.003433953 4 Saccharomyces cerevisiae LEVO FVNQHLCGSHLVEALYLVCGERGFFYTPKT P01308 singleParticle particle 7.5 ETHANE-PROPANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 1 51 10.2 55.0 1 C1 4.3 FSC 0.143 CUT-OFF RELION 3.0 326257 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 3.0 RELION 3.0 emd_10273.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -27.025113999999999 55.418995000000002 0.15409243 1.8208048 1.06 1.06 1.06 0.015 AUTHOR "manually filtered map" in paper, b-factor=-140 6CEB PDB experimental model FLEXIBLE FIT REAL emd_10273_msk_1.map emd_10273_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.051133286 0.07930375 0.0001971039 0.0018387343 1.06 1.06 1.06 AUTHOR "automatically filtered map" in paper, b-factor=-172 emd_10273_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.01014206 0.03735219 -0.000012210143 0.0017441021 1.06 1.06 1.06 AUTHOR half-map 1 post Bayesian polishing emd_10273_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 305.27997 305.27997 305.27997 90.0 90.0 90.0 X Y Z -0.011557517 0.03698443 -0.000011925838 0.0017432077 1.06 1.06 1.06 AUTHOR half-map 2 post Bayesian polishing