2024-10-16 REL PDBe PDBe PDBe 2019-08-24 2019-09-04 2020-03-11 2024-10-16 Swiss National Science Foundation CRSII3_147632 Switzerland Swiss National Science Foundation CRSII5_173709 Switzerland Swiss National Science Foundation 310030B_166672 Switzerland Cryo-EM structure of nanodisc reconstituted yeast ALG6 in complex with 6AG9 Fab Bloch JS Pesciullesi G Glycosyltransferase, Glucosyltransferase, GT-C, N-Glycosylation, MEMBRANE PROTEIN Bloch JS Pesciullesi G Boilevin J Nosol K Irobalieva RN Darbre T Aebi M Kossiakoff AA Reymond JL Locher KP Structure and mechanism of the ER-based glucosyltransferase ALG6. Nature UK 579 443 447 2020 32103179 doi:10.1038/s41586-020-2044-z 1476-4687 0006 NATUAS 6sni FULLOVERLAP Nanodisc reconstituted yeast ALG6 in complex with 6AG9 Fab Nanodisc reconstituted yeast ALG6 in complex with 6AG9 Fab 0 1 2 3 0.11289472 ALG6 1 1 Saccharomyces cerevisiae 6AG9 Fab 1 2 3 synthetic construct Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase Saccharomyces cerevisiae 0.064423223 1 Spodoptera frugiperda LEVO GPGGSTGRLAGAGGEFVDMAIGKRLLVNKPAEESFYASPMYDFLYPFRPVGNQWLPEYIIFVCAVILRCTIGLGPYSGKG SPPLYGDFEAQRHWMEITQHLPLSKWYWYDLQYWGLDYPPLTAFHSYLLGLIGSFFNPSWFALEKSRGFESPDNGLKTYM RSTVIISDILFYFPAVIYFTKWLGRYRNQSPIGQSIAASAILFQPSLMLIDHGHFQYNSVMLGLTAYAINNLLDEYYAMA AVCFVLSICFKQMALYYAPIFFAYLLSRSLLFPKFNIARLTVIAFATLATFAIIFAPLYFLGGGLKNIHQCIHRIFPFAR GIFEDKVANFWCVTNVFVKYKERFTIQQLQLYSLIATVIGFLPAMIMTLLHPKKHLLPYVLIACSMSFFLFSFQVHEKTI LIPLLPITLLYSSTDWNVLSLVSWINNVALFTLWPLLKKDGLHLQYAVSFLLSNWLIGNFSFITPRFLPKSLTPGPSISS INSDYRRRSLLPYNVVWKSFIIGTYIAMGFYHFLDQFVAPPSKYPDLWVLLNCAVGFICFSIFWLWSYYKIFTSGSKSMK DL Q12001 2.4.1.267 6AG9-Fab heavy chain synthetic construct 0.024953664 1 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO EISEVQLVESGGGLVQPGGSLRLSCAASGFNVYSSSIHWVRQAPGKGLEWVASISSYSGYTSYADSVKGRFTISADTSKN TAYLQMNSLRAEDTAVYYCAREYWSWYSYSYGIDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT 6AG9-Fab light chain synthetic construct 0.023650249999999998 1 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQ PEDFATYYCQQSYWVGYPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSG NSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC PHOSPHATIDYLETHANOLAMINE 0.0007340389999999999 5 PTY CHOLESTEROL HEMISUCCINATE 0.000486726 3 Y01 water 1.8015e-05 7 HOH singleParticle particle 7.4 ETHANE-PROPANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K3 BIOQUANTUM (6k x 4k) 2.3 1 3.0 FSC 0.143 CUT-OFF 171764 NOT APPLICABLE NOT APPLICABLE emd_10258.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 251.99998 251.99998 251.99998 90.0 90.0 90.0 X Y Z -0.09224564 0.14414388 -0.000015270758 0.0032000486 0.84 0.84 0.84 0.00768 AUTHOR Nanodisc reconstituted yeast ALG6 in complex with 6AG9 Fab