2024-05-22 REL PDBe PDBe PDBe 2019-08-18 2019-10-02 2020-06-24 2024-05-22 Israel Science Foundation 569/17 Israel European Research Council (ERC) 723991 Belgium Dunaliella Photosystem I Supercomplex Nelson N Caspy I membrane complex, photosystem I, dunaliella, light harvesting, excitation transfer, PHOTOSYNTHESIS Caspy I Malavath T Klaiman D Fadeeva M Shkolnisky Y Nelson N Structure and energy transfer pathways of the Dunaliella Salina photosystem I supercomplex. Biochim Biophys Acta Bioenerg NE 1861 148253 148253 2020 32569661 doi:10.1016/j.bbabio.2020.148253 1879-2650 6sl5 FULLOVERLAP Large dunaliella salina photosystem I-LHC supercomplex Large dunaliella salina photosystem I-LHC supercomplex 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Dunaliella salina Photosystem I P700 chlorophyll a apoprotein A1 Dunaliella salina 0.081877352 1 LEVO KVKIAVDRNPVETNFEKWAKPGHFSRALAKGPNTTTWIWNLHADAHDFDNHTSDLEEISRKVFSAHFGQLGIILIWLSGM YFHGARFSNYEGWLSDPTHIKPSAQVVWPIVGQEILNGDVGGGFQGIQITSGFFQLWRASGITSELQLYSTAIGGLVLAA ACFFAGWFHYHKAAPKLEWFQNVESMLNHHLAGLLGLGSLAWAGHQIHVSLPVNKLLDAGVDPKEIPLPHEFLLNQSIIA DLYPSFSKGLAPFFTLNWAEYSDFLTFKGGLNPVTGGLWLSDTAHHHLAIAVLFLVAGHQYRTNWGIGHSIKDILESHKG PFTGNGHAGLYEILTTSWHAQLAINLALFGSLSIIVAHHMYAMPPYPYLATDYGTQLSLFTHHMWIGGFCVVGAGAHAAI FMVRDYDPTNNYNNLLDRVIRHRDAIISHLNWVSIFLGFHSFGLYIHNDTMSALGRPQDMFSDTAIQLQPVFAQWIQNTH FTAPQLTAPNALAATSLTWGGDVVAVGGKVAMMPIALGTSDFLVHHIHAFTIHVTVLILLKGVLFARSSRLIPDKANLGF RFPCDGPGRGGTCQVSAWDHVFLGLFWMYNSLSIVIFHFSWKMQSDVWGTVTDSGVSHITGGNFAQSANTINGWLRDFLW AQSSQVIQSYGSALSAYGLMFLGAHFVWAFSLMFLFSGRGYWQELIESIVWAHNKLRVAPSIQPRALSITQGRAVGVAHY LLGGIATTWSFFLARIIAVG D0FXV2 1.97.1.12 Photosystem I P700 chlorophyll a apoprotein A2 Dunaliella salina 0.08167143799999999 1 LEVO TKLFPKFSQGLAQDPSTRRIWYGLATAHDFESHDGMTEENLYQKIFASHFGQLAIIFLWTSGNLFHVAWQGNFEQWVTDP IHVRPIAHAIWDPHFGQPAVEAFTRGGASGPVNIATSGVYQWWYTIGLRSNQELYVSSVFLALVSAVFLFAGWLHLQPNF QPSLSWFKDAESRLNHHLAGLFGVSSLAWTGHLVHVAIPESRGQHVGWDNFLSVLPHPQGLTPFWSGNWAAYAQNPDTAS HAFGTADGSGTAILTFLGGFHPQTQSLWLSDMAHHHLAIAVLFIVAGHMYRTNFGIGHRLEAILEAHTPPAGGLGAGHKG LFHTVNNSLHFQLGLALASVGTITSLVAQHMYSLPPYAYLAVDFTTQASLYTHHQYIAGFIMCGAFAHGAIFFIRDYDPE QNKGNVLARVLDHKEAIISHLSWVSLFLGFHTLGLYVHNDVVQAFGTPEKQILIEPVFAQWIQAAQGKSLYGFDLLLASS SSPAYSAGQSLWLPGWLEAINNNQNSLFLTIGPGDFLVHHAIALGLHTTTLILVKGALDARGSKLMPDKKDFGYSFPCDG PGRGGTCDISAYDAFYLAVFWMLNTIGWVTFYWHWKHLTLWQGNVSQFDESSTYLMGWLRDYLWLNSSQLINGYNPFGMN SLSVWAWTFLFGHLVYATGFMFLISWRGYWQELIETLVWAHEKTPLANLVYWKDKPVALSIVQARLVGLAHFSVGYIFTY AAFLIASTAGRFG D0FXZ0 1.97.1.12 Photosystem I iron-sulfur center Dunaliella salina 0.00871709 1 LEVO AHVVKIYDTCIGCTQCVRACPLDVLEMVPWDGCKAAQMASSPRTEDCVGCKRCETACPTDFLSVRVYLGNESTRSLGLAY D0FXW7 1.97.1.12 PsaD Dunaliella salina 0.016214705 1 LEVO PWKQPELDPDTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMRKGPNLLKFARKEQCMALTTQLRSK FRQTPCFYRVYADGKVQYLHPKDGVYPEKVNAGRVGVNQNMRSIGKNVDPIKVVKFTGSAPFEI PsaE Dunaliella salina 0.007297158 1 LEVO EIGPKRGSLVKVLRPESYWYNQVGKVVSVDQSGIRYPVVVRFENQNYAGVSTNNYALDEIEEVK PsaF Dunaliella salina 0.018115789 1 LEVO DIAGLTPCSESKAYNKLERKELKVLDKRLKQYEPGSAPYLALQATKERTENRFKTYAKQGLLCGNDGLPHLISDPGLALR FNHAGEVFITFGFLYVAGYIGHVGRQYIILSKEDAKPTDKEIILDVPLALKLAFQGWAWPLASIQELRNGSLLEKDENIT VS Photosystem I reaction center subunit IX Dunaliella salina 0.0046143619999999995 1 LEVO MKDFTTYLSTAPVVGLGWAIFTSGLLIEINRFFPDPLVFSF D0FXW0 PsaG Dunaliella salina 0.010928419 1 LEVO LEPAVAIGGSTVSFLALGRFVFLPYQRRRTEMEVGPGRLGPKTTGDTFFDRLQKPASFVETKSKDPSGFGGWHAALGHVF GYFLLACSSLQDAGQVIAHWG PsaH Dunaliella salina 0.010012246999999998 1 LEVO KYFDVQDLENTTGAWDLYGVDEMKRYPGLQEEFFQRATDAVSRREALNGFVALSAGVASIALFGKGASTLELPIGTKGPR MEKTENGKGGIL PsaI Dunaliella salina 0.004236944 1 LEVO ATEGYPFVPPDWAPALFVPLTGLVLPAVGMAWAFTYIQK PsaK Dunaliella salina 0.008191218 1 LEVO GQSGSVRAIMVSSIGACLFASRFGLAPSVRKNATAAGTDLEKSVHAAGDDPGAGFTATDVLAMGAAGHAIGVGEWLAQLA RGGV PsaL Dunaliella salina 0.016189838999999998 1 LEVO QQVIQPLNGDPFVGMLETPVTSAPIVANYLSNLPAYRTGVAPNLRGVEIGLAHGFLLAGPFIKLGPLRDVPGTAEVVGCM SAALVLILALCLSLYGNAAFQNQPSMGKKTLSGRPLPQDPLMSEEGWAKFAAGFTVGGLSGVAWAYILTQVCLGR PsaO Dunaliella salina 0.009635004 1 LEVO LRVDPIVPAISFVGWTLPSNIGTSALNGQSLFGAFYESIGQNLAHWPTGFALDDKFWLYMVTWHTGLFIVMLLGQVGFKG RTEDYF Chlorophyll a-b binding protein, chloroplastic Dunaliella salina 0.021305229999999998 1 LEVO QRAGNFAPGSEPKEYLNDLPGNFNFDPLELGKEKGTLQRYREAELIHCRWAMLGAAGCLAVEVLGLGNWYDAPLWAVTGD KPTWFGIEVPFDIATILGVEVVAMAVAEGLRNDNQDMEKRLYPGGAFDPLGFSKDPKSFEDKKLKELKNGRLAMVACLGF AGQHAATGKPILAALGDHLSSPFFNNFATNGVSVPGV C1K003 Lhca2 Dunaliella salina 0.022813821999999997 1 LEVO DRPLWSPGSEPPAWLDGSLAGDYGFDPLHLSEEPEMRKWMVQAELVHCRWAMLGVAGILFTSIGAKAGGNFPDWYDAGKE LQKNSDIPLGSLIFTELLLFGWVETKRLYDLRNPGSQGDGSFLGITDGLKGKENGYPGGLFDPMGMSKNEASFKEAKQKE VKNGRLAMLAFVGFIAQHHATHKSPIDNLLDHVADPFHVTFATNGVSI Chlorophyll a-b binding protein, chloroplastic Dunaliella salina 0.02458591 1 LEVO SKDFLYVGSDAAALKYLDGTLPGDYGFDPLGLLDPTVSNGQGAGGFVNPRWLQYSEVIHARWAMLGAAGCIAPEILGKAG VIPAETAVDWFRTGVIPPAGVYKDFWADPFTLFFIEVVAIQFAELKRLQDYKNPGSQSRQYFLGLEGLFKGSDNPAYPGG PFFNFANFGKTEAEMKKLKLNEIKNGRLAMLAMFGYGAQAVITGDGPFDNLLAHLADPTGANLITNLG Lhca4 Dunaliella salina 0.023131030999999996 1 LEVO DRPLWYPGATPPAHLDGSMLGDYGFDPLRLGTNPDRMKWFREAELTNGRWAMAAVVGILFTDVFTSIGLVGLPKWWEAGA QTYPIDNQTLRTLAIIEFLLFGWVETKRLYDLRNPGSQGDGSFLGITDGLKGTENGYPGGIFDPLGYSKTSPEKLDELQN GRLAMLAFLGFASTAAVNGQGPIESLQTHLADPFHVTFATNGVSIPHFTEF Lhca5 Dunaliella salina 0.021676771 1 LEVO LPAIPLADVQSLSYLDGHLPGDMGFDPLHLGSGVLSQDWLRYAEVVHGRWAMLGVVGCLTPEALAMRGTIPPERGVEDNQ TLLIIEIAVFSFLESKRYEGYKKTGEGGFINSYPFDPVGLNSPKHAVNELQQNGRLAMLAFLGFASTAAVNGQGPIESLQ THIADPAHNNVFTSSVGKESCVFVAVLSILPMLIEANKALGK Lhca6 Dunaliella salina 0.019559611 1 LEVO LPDVIPPPHLNGTLPGDSFDPLGLGLNEERLKWSVTMGKTNCRWAMMAVTGIMGQELLGVPVKWFEAGAAEYDLPVQAQV PILFLVMGFLETKRFQGFRESGFINSYPFDPVGLNSPKHATKEVKNGRLAMVAFVGFAVQALVTRTQPIEGLQKHLADPF GKNITYYLTHTPEVIAGT CHLOROPHYLL A ISOMER 0.000893489 1 CL0 CHLOROPHYLL A 0.000893489 172 CLA PHYLLOQUINONE 0.000450696 2 PQN IRON/SULFUR CLUSTER 0.00035163999999999996 3 SF4 BETA-CAROTENE 0.000536873 31 BCR 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE 0.00072297 15 LHG octadecanal 0.000268478 1 OCD DODECYL-ALPHA-D-MALTOSIDE 0.000510615 4 LMU DIGALACTOSYL DIACYL GLYCEROL (DGDG) 0.0009492989999999999 4 DGD 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE 0.000704998 4 3PH CALCIUM ION 4.0078e-05 1 CA 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE 0.0007871579999999999 8 LMG Tripalmitoylglycerol 0.00080732 2 4RF PHOSPHATIDYLETHANOLAMINE 0.0007340389999999999 9 PTY (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL 0.000568871 9 LUT (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL 0.00060087 6 XAT CHLOROPHYLL B 0.000907472 13 CHL 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL 0.0007951159999999999 3 SQD trimethyl-[(2~{R})-1-oxidanyl-1-oxidanylidene-4-[(2~{S})-2-[(1~{S})-1-oxidanyloctadecoxy]-3-[(1~{R})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium 0.0007732409999999999 2 LMK [(2~{R})-1-nonanoyloxy-3-[oxidanyl-[(2~{R},3~{S},5~{R},6~{R})-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl] (5~{Z},8~{Z})-heptadeca-5,8-dienoate 0.000722841 2 P3H singleParticle particle 8.0 Quantifoil R1.2/1.3 COPPER GLOW DISCHARGE ETHANE 100 277 LEICA EM GP
2.5 sec blotting before plunging.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.9 3.0 GATAN K2 SUMMIT (4k x 4k) COUNTING 1 4306 42.68 1 720711 6RHZ
Minimal dunaliella salina photosystem I complex
 2 C1 2.84 FSC 0.143 CUT-OFF RELION 3 189006 ANGULAR RECONSTITUTION RELION 3 ANGULAR RECONSTITUTION RELION 3 12 27366.0 RELION 3 emd_10236.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.07073394 0.15494287 0.00016259018 0.005252027 1.067 1.067 1.067 0.0247 AUTHOR AB INITIO MODEL REAL 83.0 emd_10236_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.024789143 0.094956614 0.00016287049 0.00264271 1.067 1.067 1.067 AUTHOR emd_10236_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 384.12003 384.12003 384.12003 90.0 90.0 90.0 X Y Z -0.024185926 0.099004656 0.00016230988 0.0026451773 1.067 1.067 1.067 AUTHOR