2024-05-22RELPDBePDBePDBe2019-08-152020-07-292020-07-292024-05-22Cryo-EM Structure of T. kodakarensis 70S ribosome in TkNat10 deleted strainMatzov DSas-Chen AT. kodakarensis, ac4C deletion, Ribosome, cryo-EMSas-Chen AThomas JMMatzov DTaoka MNance KDNir RBryson KMShachar RLiman GLSBurkhart BWGamage STNobe YBriney CALevy MJFuchs RTRobb GBHartmann JSharma SLin QFlorens LWashburn MPIsobe TSantangelo TJShalev-Benami MMeier JLSchwartz SDynamic RNA acetylation revealed by quantitative cross-evolutionary mapping.NatureUK583638643202032555463doi:10.1038/s41586-020-2418-21476-46870006NATUASEMD-10223other EM volume6skgFULLOVERLAP70S ribosome from Thermococcus kodakarensis70S ribosome from Thermococcus kodakarensis01234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950515253545556575859606162636465Thermococcus kodakarensis16S ribosomal RNAThermococcus kodakarensis0.4873949381AUUCCGG(OMU)UGAUCCUG(5MC)CGGAGGCCACUGCUAUGGGGGUCCGACUAAGCCAUGCGAGUCAUGGGGCGCGCUC
UGCGCGCACCGGCGGACGGCUCAGUAACACGUCGGUAAC(OMC)UACCCUCG(OMG)GAGGGGGAUAACCCCGGGAAACU
GG(OMG)GCUAAUCCCCCAUAGGCCUGAGGUACUGGAAGGUCCUCAGGCCGAAAGGGGCAUCUGCCCGCCCGAGGAUG
(OMG)GCCGGCGGC(OMC)GAUUAGGUAGUUGGUGGGGUAACGGCCCACCAAGCCGAAGAUCGGUACGGGCCAUGAGAGU
GGGAGCCCGGAGAUGGACACUGAGACACG(OMG)GUCCAGGCCCUACGGGGCG(5MC)AGCAGGCG(5MC)GA(A2M)AC
CUCCGCAAUGCGGGCAACCGCGACGG(OMG)GGGACCCCCAGUGCCGUGGCAUAGCCACGGCUUUUCCGGAGUGUAAAAA
GCUCCGGGAAUAAGG(OMG)CUG(OMG)GCAAGG(5MC)CGGUGG(5MC)AGCCGCCG(5MC)GGUAA(OMU)ACCGGCG
GCCCGAGU(OMG)GU(OMG)GCCGCUAUUAUUGGGCCUAAAGCGUCCGUAGCC(OMG)GGCCCGUAAGUCCCUGGCGAAA
UCCCACGGCUCAACCGUGG(OMG)GCUUGCUGGGGAUACUGCGGGCCUUGGGACCGGGAGAGGCCGGGGGUACCCCUG
(OMG)GGUAGG(OMG)GUGAAAUCCUAUAAUCCCAGGG(OMG)GACCGCCAGU(OMG)G(5MC)GAAGGCGCCCGG
(5MC)UGGAACGGGUCCGACGGUGAGGGACGAAGGCCAGGGGAGCGAACCGGAUUAGAUACCCGGGUAGUC(OMC)
(OMU)GGCUGUAAAGGAUGCG(OMG)GCUAGGUGUCGGGCGAGCUUCGAGCUCGCCCGGUGCCGGAGGGAAGCCGUUAAG
(OMC)CCGCCGCCUG(OMG)GGAGUACGGCCGCAA(OMG)G(5MC)UGAAACUUAAAGGAAUUGGCGGGGGAGCACUACA
AG(OMG)GGUGGAGCGUGCGGUUUAAUU(OMG)GAU(5MU)(LV2)AACG(5MC)(OMC)GGGAACCUCACCGGGGGCGA
CGGCAGGAUGAAGGCCAG(OMG)CUGAAGGUCUUGCCGGACACGCCGAGAGGA(OMG)(2MG)UGCAUGG(5MC)(5MC)
G(5MC)(5MC)GUCAGCUCGUAC(OMU)GUGAGGCGUCCACUUAAGUGUGGUAACGAGCGAGACCCGCGCCCCCAGUUGC
CAGUCCUCCCCGCUGGGGAGGAGGCACUCUGGGG(OMG)GACCGCCGGCGAUAAGCCGGAGGAAGGAGCGGGCGACGGUA
GGUCAGUAUGCCCCGAAACCCCCGGGCUACACGCG(5MC)GCUACAAUGGGCGGGACAAUGGGAUCCGACCCCGAAAG
(OMG)GGAAGGGAAUCCCCUAAACCCGCCCUCAGUUCGGAUCGCGGG(5MC)UGCAACUCGCCCGCGUGAAGCUGGAAUC
CCUAGUACCCG(5MC)GUGUCAUCAUCGCG(5MC)GGCGAAUACGUCCCUGCUCCUUGCACACAC(5MC)G(OMC)CCG
(OMU)CA(OMC)UCCACCCGAGCGGGGUCCGGGUGAGGCCUGGUCUCCCUUCGGGGAGGCCGGGUCGAGCCUGGGCUCCG
UGAGGGGGGAGAAGUCGUA(6MZ)CAAGGUAGCCGUAGGGG(MA6)(MA6)CCUACGG(5MC)U(5MC)GAUCACCUCCU
AUCGCCGGA30S ribosomal protein S2Thermococcus kodakarensis0.0230397931LEVOMEEYLVPLDQYLAAGVHIGTQQKTKDMKKFIYRVRQDGLYVLDVRKTDERLRVAGKFLAKFDPESILAVSVRLYGQRPVK
KFGEVTGAKAIPGRFLPGTMTNPQVKNFIEPDVLIVTDPRADHQALKEAVEIGIPIVALVDTENFLSYVDIAIPTNNKGR
KALALIYWILAREVLYNRKEIESREDFKIPVEDFEMRIIRTQ5JJD230S ribosomal protein S3Thermococcus kodakarensis0.023390161LEVOMAIERYFIKEGVKEMLIDEFLEKELRRAGYGGLDIKKTPLGTKVTIFAANPGYVIGRGGRRIRELTRILEKQFGLENPQI
EVEEIKNPYLNAKVQAVRLAQALERGIHFRRAAYSAIRAIMRNGARGVEIRLSGKLTGERAKSVRFYQGYLAKVGNPAET
LVSRGYAQAQLKLGVIGVKVSIMPPDAKLPDEIEIKEIVEEEVSANEAQQ5JDH530S ribosomal protein S3AeThermococcus kodakarensis0.0230649199999999961LEVOMARGNPRKRAAAAKDKWKMKEWYIVYAPDFFGSKEIGLTPADDPEKVIGRVIETTLKDLTGDFTKGHVKLYFQVYDVKGQ
NAYTKFKGHTLARSYIRSLVRRRTTRVDGIFNITTKDGYKLRVMGMVIAYRRIQTSQERAIRKIIQDIIYKKAEELNFAD
FVLQSVNGQIASEIAKEARKIYPIKRAEVRKIKVLAEPSAQ5JGM430S ribosomal protein S4Thermococcus kodakarensis0.0212406209999999981LEVOMGDPKRQRKKYETPSHPWIKERLDRERVLKRNYALKNKKELWRHETQLKEFRRRARRLLAARGKQAEIERQQLLQRLYRL
GLLPADAVLDDVLSLTVEDVLERRLQTIVYRKGLARTMKQARQLIVHGHIEVNGQVIRSPGYLVLREEEDTITYAKGSPF
AKEGHPERMVIEQAKQGGEAQ5JJF230S ribosomal protein S4eThermococcus kodakarensis0.0278206291LEVOMARKGPKRHLKRLAAPTSWYIHRKAYKWAVRPSPGPHSMKTSIPLIYIVRDYLGYAKTAREARKILNEGKILVDGRVRKD
YKFPVGIMDVVSIPETGEHYRVLPNRIGKLILHPISEEEAKLKPFRINNKRMVKGAKVQLNLHDGSNHLVSLAEKDAYKT
SYTVIMQVPERQIVKVLPFEVGAYVFVTQGKNVARKGKIVEVRQFPMGWPDVVTIEDENGELFDTLKEYAFVIGKDKPEI
SLPQ5JJG030S ribosomal protein S5Thermococcus kodakarensis0.0263726131LEVOMSDPREIAQRVLEEWEPKTKLGRLVKEGQITDIHEIFRKGYQIKEPEIVDVLLPEVNLRENQEVLDIALTVRMTDSGRRI
RFRVLAAVGNRDGYVGLGIGHGREVGIAIRKAINYAKMNIIEIKRGCGSWECRCRRPHSIPFAVEGKEGSVRVKLMPGPR
GLGLVIGDVGKKILTLAGVQDVWSQTLGETRTTVNFAKAVFNALYNTNRVAIKPEDIERYGIVVGRAMPTTFEVEQ5JJG830S ribosomal protein S6eThermococcus kodakarensis0.0137609861LEVOMATFKLVISNPKNGVAKQVEISGPEADKLIGRRIGEEIPASELGLNLSEIFGEEIPADAKLKITGGTDKDGFPMRPDVHG
PRRVKILLSRGPGFRPRERGERRKKTVHGNTISPNIVQVNMKIVFQ5JDK830S ribosomal protein S7Thermococcus kodakarensis0.0245737129999999971LEVOMAKPLTERFFIPKEVKVMGRWSVEDVTVEDPSLKPYINLDARILPHSHGRYAKKPFGKANVHIVERLINKVMRSGASHYK
AGGHFMRREHRSIMSKKMKAYEVVKEAFMIIERRTKQNPIQVLVKAIENSAPREDTTTIAFGGIRYHMSVDVSPLRRLDI
ALKNIALGASIKCYRNKTTYAQALAEEIIAAANKDPKSFAYSKKEEIERIAQSSRQ5JE0430S ribosomal protein S8Thermococcus kodakarensis0.0146021091LEVOMTLLDPLANALSHITNSERVGKKEVYIKPASKLIGEVLRVMLENGYIGEFELIDDGRAGIYRVQLIGKINKAGAIKPRFP
VKARDYEKWEKRFLPAFEFGILIVSTSQGVMTHKEALEKGIGGRLIAYVYQ5JJG330S ribosomal protein S8eThermococcus kodakarensis0.0145480311LEVOMAIWQGRSLKKPSGGRIILARKKRKRELGREPANTKVGEDREKRKIIRTYGGNRKVRLVEALYANVFEGGKGKKVKILNV
VENPANRQYARRNIITKGAIIETEIGKAIVTSRPGQDGVVNAVLIKEENAQ5JGF330S ribosomal protein S9Thermococcus kodakarensis0.0153589429999999981LEVOMRVIQTAGKRKTAVARATIREGKGRVRINHKPVEIIEPEIARFTIMEPLILAGEEIVSRVDIDVKVEGGGFMGQAEAARV
AIARALVEWTNDMNLKEKFMKYDRTMLVGDSRRTEPHKPNRSTKGPRAKRQKSYRQ5JJE230S ribosomal protein S10Thermococcus kodakarensis0.0117166659999999991LEVOMQKARIKLASTNIKALNEVTDQIKQIAERTGVRMSGPIPLPTKRIRITTRKSPDGEGSATFDRFELRVHKRLVDIEADER
AMRQIMRIRVPEDVTIEIELISQ5JFZ530S ribosomal protein S11Thermococcus kodakarensis0.0151112411LEVOMSEEQTTQVNIKKKEKWGVAHIYSSYNNTIIHITDLTGAETISRWSGGMVVKADRDEPSPYAAMIAARRAAEEAMEKGIT
GVHIKVRAPGGSKSKSPGPGAQAAIRALSRAGLRIGRVEDVTPIPHDGTRPKGGRRGRRVQ5JJF330S ribosomal protein S12Thermococcus kodakarensis0.0164885371LEVOMAGKKAPYGEFAGRKLKLKRKKFRWSDIRYKRRVLRLKEKSDPLEGAPQARGIVLEKIAVEAKQPNSAMRKAVRVQLIKN
GKVVTAFTPGDGAINHIDEHDEVIIEGIGGPKGGSMGDIPGIRYKVVKVNRVSLKELVKGRKEKPRRQ5JE2030S ribosomal protein S13Thermococcus kodakarensis0.0170258751LEVOMTENFRHIVRVAGVDIDGHKQVRWALTGIKGIGINFATMVLRVAGIDPFMKAGYLTDEQVKKIEEILADPVAHGIPAWAV
NRPKDYETGKDMHLITAKLVMAWREDVNRLRRIRAYRGIRHELGLPVRGQRTRSNFRHGTTVGVSRRKKQ5JJF130S ribosomal protein S15Thermococcus kodakarensis0.0175837849999999981LEVOMARIHARKRGKSGSKRPPRTAPPAWVEYTAEEVEALVVKLRKEGYSTAMIGTILRDQYGIPSVKLITGKKITKILEENGL
APEIPEDLMFLIRRAVNLRKHLEQHPKDKHSRRGLQLIESKIRRLVKYYRRTGKLPAKWRYDPEQAKLLVRQ5JGJ430S ribosomal protein S14 type ZThermococcus kodakarensis0.0066340881LEVOMAKADYNKRKPRKFGKGARRCMRCGQYGPIIRIHGLMLCRHCFREVAPKLGFKKYEQ5JJG230S ribosomal protein S17Thermococcus kodakarensis0.0132193851LEVOMREIGLRVQPPAEVCNDPKCPWHGNLKIHGRYVEGIVVSDKGKKTVVVERQYYHYLKKYERYELRKSKVHAHNPECINAK
VGDKVLIAETRPISKTKSWVVVAVLQRAERAEEVQ5JDH930S ribosomal protein S17eThermococcus kodakarensis0.0080364309999999982LEVOMGNIKQTFIKRTARELFDRYPDKFTRDFEHNKKMVAELTNVTSKTIRNRIAGYITRLVRMKEEGKILQ5JDC730S ribosomal protein S19Thermococcus kodakarensis0.0154533739999999991LEVOMARKKEFRYRGYTLDELLNMSLEEFAKLLPSRQRRSLKRGLSPEQKKLLRKIRLAKKGKYNKPIRTHSRDMIVLPEMVGM
TIHVYNGKEFVPVEIKEEMIGHYLGEFAMTRKVVQHGSPGVGATRSSMFVAVKQ5JDH330S ribosomal protein S19eThermococcus kodakarensis0.0173039769999999981LEVOMATVYDVPGDLLVERVAKALKEVPEIKPPEWAPFVKTGRHKERLPEQEDWWYYRVASVFRKVYIDGPVGIERLRTWYGGR
KNRGHAPEHFYKAGGSIIRKALQQLEAAGFVQKVPGEGRIVTPKGQSFLDKIATELKKELEEQIPELKKYQ5JGN530S ribosomal protein S24eThermococcus kodakarensis0.0114581159999999992LEVOMEIKVTEIRENKLLGRKEIYFDVLHEGEPTPSREAVKGKLVAMLDLDPNTTVIQYIRSYFGSNVSKGYAKAYETRERMLY
IEPEYILVRDGLVQKQEEQ5JIY830S ribosomal protein S27eThermococcus kodakarensis0.0070855141LEVOMALPKNLIPMPRSRFLRVKCIDCGNEQIVFSHPATPVRCLVCGATLVEPTGGKGIIKAKVLEVEEQ5JE5030S ribosomal protein S28eThermococcus kodakarensis0.0079712439999999991LEVOMSDEGYPAEVIEIVGRTGVTGGVTQVKVRILEGRDKGRVIRRNVKGPVRVGDIVILRETEREAREIRRRRQ5JGR4Predicted zinc-ribbon RNA-binding protein involved in translationThermococcus kodakarensis0.0070012471LEVOMFAVEMKFEVPVCTSCGKEITPREHATHFVCPNCGEAIIWRCESCRVLSVPYKCPKCGWEGPQ5JFE223S ribosomal RNAThermococcus kodakarensis0.98798143799999991AACGUCCCGCCCGGUGGCAACUAAGCCGCCUGGUGGAUGGCUCGGCUCGGGGCG(5MC)CGACGAA(OMG)G(OMG)
(OMC)GUGGCAAGCUGCGAUAAGCCCCGG(OMC)GAGGCGCAGGCAGCCGUCGAACCGGGGAUGCCCGAAUGGGACCUCC
CGCGGCUUUUGCCGCACUCCCAGUCGGGAGGGGGAACGCGGGGAAUUGAAACAUCUUAGUACCCGCAGGAAAAGAAA
(OMG)CAAAAGCGAUGCCGUUAGUAGGGGCGACCGAAAGCGGCACAGGGCAAACUGAACCCCGGGCCGAAAGGUUCG
(OMG)GGGAUGUGGUGUUGUAGGGCCCGCGGAGGAGCCCUGGGGGCGAAGCCGAAGUC(OMC)GCUGGAACGCGGCGCCG
GAGAG(OMG)GUGAAAGCCCCGUAGGCGUAAGCCCCCGAGCUCCUGCGGUGUCCCUGAGUACCGUCGGUCGGAUAUCCGG
CGGGAAGCUGGGAGGCA(OMU)CGGCUCCCAACCCUAAAUACGUCCCGAGACCGAUAGCGAACUAGUACCGUG(A2M)G
(OMG)GAAAGCUGAAAAGCACCCCGGGAGGGGGGUGAAAAGAGCCUGAAACCAGGCGGCGAUAGGAGGGUGCGGCCCGAA
A(OMG)GGUUGACCCUCCCCGAAGGAAACACGGGCGACCGUGGAGUACGAGGGGAGGUGACCGGGGUUGCACCGUCCG
(OMU)CUUGGAUCACGG(OMG)GCA(OMG)GGAGUUCACCCGAGCGGCGAGGUUAAGGGGGUCAACCCCGAAGCCGCAGG
GAAACCGACAGGUCCGCAGCCCGUAAGGGCGAGGGACGGGGUGUGAAAGCGCCCGGAGUCGCUCGGGUGAGACCC(OMG)
AAGCCGGUCGAUCUAGCCCGGGGCAGGG(5MU)GAA(OMG)UCCCUCAACAGAGGG(A2M)UGGAGGCCCGCUAGGGGUG
(OMC)UG(OMG)CG(5MU)GCA(A2M)UUCGCUCCCGUGACCCCGGGCUAGGGGUGAAA(OMG)GCCAAUCGAGGCCGGC
GAU(A2M)GCUGGUUCCCGCCGAAUUAUCCCCCAGGAUAGCCCGGCCGGAGGUAGGCAGUGGGGUAGAGCACUGAUUGGG
GGUUUAGGGGGCGAAAGCCUCCGGCUCCCUGUCAAACUCCGAACCCACUGCCGCCGUAGAUGGCCGGAGUAGGGGGGCGG
UGUAAGCCGUCACCCGAGAGGGGAACAACCCAGACCGGGGUUAAGGCCCCAAAGUGCCGGCUAAG(OMU)GUUACUCCAA
AGGGUGUCCCUGGCCUUAGACAGCGGGGAGGUAGGCUUAGAAGCAGCCAUCCUUUAAAGAGUGCGUAACAGCUCACCCGU
CGAGGUCAGGGGCCCCGAAAAUGGACGGGGCUUAAGCCGGCCGCCGAGACCCCGGCGCACGGACCGAUUGGUCCGUGAUC
GGGUAGGC(OMG)GGCGUGCCGAUGGGG(5MC)GGAAGCCGGGCCGUGAGGUCCGGUGGACCCGUCGGUAUUGCGGAUCC
UGCCGGGAG(OMU)AGCAGCAUAGCCGGGUGAGAAUCCCGGCCGCCGUAGGGGCCAGGGUUCCACGGCAAUGUUCGUCAG
CCGUGGGUUAG(OMU)CGGUCCUAACCCGACCCGUAACUCGGC(OMG)UCGGGGAAAGGGAAAC(OMG)GGUUUAUAUUC
CCGUACCGCGGUGGUAGGUGCGGCAACGCAAGCCCGAGGGGUGACGCCUCGGGGUAGGCGGACCGGUCCACAAGGCCGGC
UAAGCGUAUAAGCCCGGGGAGUGCCGUAAUGGCGAGAACCGGGUGAAAGCGCGAAUGGCCUCCCGUAAGGGGGGUUCCGC
CGAUCCCUGGGGCCCGUGAAAAGCCCCUCGGGAACGAUCCACCGCGACCGUACCGAGAACCGACACUGG(5MU)GCCCCU
GGG(OMU)GAGAAGCCUAAGGCGUGUCGGGGGAAACCCAGCCGAGGGAACU(OMC)GGCAAAUUGGCCCCGUAACUUCGG
GAGAAGGGG(5MU)GCCUGCGGGUGUGUAACCCGCAGGUCGCAGUGACUAGG(OMG)GGGCCCGACUGUUUAGUAAAAAC
ACAGGUCCCAGCUAGCCCGAAAGGGUUUGUACUGGG(OMG)CC(OMG)ACGCCUGC(5MC)CAG(OMU)GCCGGUAUGUG
AAGCCCGGGUACAA(OMC)CGGGUGAAGCACCG(OMG)UAAAC(OMG)GCGGGGGUAACUAUAACCCUCUUAAGGUAG
(OMC)GAAAUUC(5MC)UUGUCGGUUAAAUG(5MC)CGAC(5MC)UGCAUGAAUGGCGUA(A2M)CGAGGUCCCCACUGU
(OMC)CCCGGCUGGGGCCCGG(OMC)GAAACCUCUGCCAGGCGCAUAUGCCUGGGACCUCCGGUGGGAA(OMG)(OMC)G
AAGACCCCAUGGAGCUUUACUGCAGCCUGCCGUUGCCGUACGGCGGGGGGUGCGCAGCGUAGGCGGGAGGCGUCGAAGCC
CGCCCUCCGGGGCGGGUGGAGCCGCCCAUGAGACACCGCCCACCCUCUGCCGUACGGCUAACCCGCGGAAGCGGGGAC
(A2M)GCGGUAGGUGGGCAGUUUGGCUG(OMG)GGCGGCACACCCUCGAAAAGGUAUC(OMG)AGGGUGCCC(OMU)AAG
GUCGGCUCAGGCGGGUCAGGAAUCCGCCGUAGAG(5MU)GCAAGGGCAAAAGCCGGCCUGACUGGACCCGUAACAGA
(OMG)GC(OMG)GGUCCAGCCGCGAAAGCGUGGCCUAGCGAACCCCUGUGCCUCCCCGGUGGG(OMG)GCCAG(OMG)
(OMG)AUGACAGAAAAGC(OMU)AC(OMC)CUGG(OMG)GA(4SU)AACAGAGUCGUCUCGGGCGAGAGCCCA(4SU)AU
CGACCCCGAGG(OMC)UUGCUACCU(5MC)GCUGUCGGCUCUUCCCAUCCUGGCCCUGCAGCAG(OMG)G(OMG)CCAAG
GGUGGGGG(OMU)GUUCACCCAUUAAAG(OMG)GGAACGUGAGCUGGG(5MC)UUAGACCGUCGUGAGACAGGUCGGAUG
CUAUCUACCGGA(OMG)GUGUUGGCCGCCUGAG(OMG)GGAAGGCUCCCCCAGUACGAGAGGAACAGGGAGCCGCGGCCU
CUGGUCUACCGGUUGUCCUACAGGGCAUAGCCGGGCAGCUACGCCGCAGCCGAUAAGGCCUGAAAGCAUCUAAGGCCGAA
GC(OMG)GCCCCCGAAAACAGGCGGCCACUCCUGGGCGUUGGGCCUGGGCGACCGGUCCGUUGCCCAGGACG(A2M)GGG
CCCGGGAAGAAGACCCGGUUGAUGGGG(OMC)GGGGAUGUAAGCGGGAAGGGAAACCGACCCGUUCAGUCUGCCGCUCCC
AACAGCCCGAGUGCUACCGGGC5S ribosomal RNAThermococcus kodakarensis0.0407443091GGUACGGCGGCCAUAGCGGCGGGGCCACACCCGGUCUCGUUUCGACCCCGGAAGUUAAGCCCGCCAGCGUUCCCGGGUGU
ACUGCCCUCCGAGAGGGGGCGGGAAACCGGGAACGCCGCCGGCCACAP006878.150S ribosomal protein L2Thermococcus kodakarensis0.0260924321LEVOMGKSLIQQRRGKGTTTFRAPSHRYRGAVRYVPLNLVQERTLRGVVEEILHDPGRTAPVARVKFEDGTKKLIIAPEGVLVG
QEIYIGPEAPIAIGNTLPLAKIPEGTYVYDIEGVPGDGGKYVRAGGTYALVVSREKDKVIVQLPSGELKAFNPMCRATIG
VVAGGGRLEKPIVKAGKAYYIAKARNRFWPKPRGVKMNAVNHPHGGKEHHIGRPSTVSRRAPPGRKVGHIAARRTGRRKQ5JDH250S ribosomal protein L3Thermococcus kodakarensis0.0391186371LEVOMGKIHRPRRGSLAYSPRKRAKSIVPRIRSWPKDSEVRMLGFAGYKAGMTHILMIDDSPGLTKGKEIFVPVTIVEVPPLFV
YGIRAYKQGYLGLETATEVWFHDLHKNVARRIKTLPKNYNEEAFQAKLGQLEDLVNDGEIADVRLLVHTQPWLIGLKKKP
EVMEYAIGGDDVKAKFDYAKEKIGKELRAGEVLHEGELLDVIAVTKGKGTQGPVKRWGVKVQFHKAQRAGKGRHIGNLGP
WHPARVMWTVPQAGQMGFHHRTEFNKRLIAIGENGKLVLDGNEIEITPKGGFPHYGIVRSDFLMIEGTIPGSFKRIIRVR
PAIRPPKKKPPVERPQITYVSRESKQQ5JDJ050S ribosomal protein L4Thermococcus kodakarensis0.0287686041LEVOMKVKVFNLEGEPVEEIELPKVFSTPFRPDLIRRAVIASWTHRIQPQGRDPQAGKRRVTENIGKGHGMARVERIKTSPRFA
AFVPFAVGGRRTHPPKVEKIIWEDINKKERRLAIMSAIAATANYDLVRARGHVVDNVVQIPLVVTDDLQKVFKTAQTREI
FKKLGVWDDIERAKKNTKIRAGKGKMRGRRYKKAKGPLIVVAKNEGIVQGARNHPGVDVVTVDNLGVEYLAPGTHPGRLT
IWTKGAIERLREIYGQ5JDI050S ribosomal protein L5Thermococcus kodakarensis0.021027571LEVOMQINREAILADWEAHPMRKPRIAKVTINIGVGESGERLTKAETMLEQLVGQKPIRRRAKQTNRDFGIRRGEPIAVKVTLR
GEKAYQMLDRLLEAVDRKLKASNFDEHGNFCFGIQEHINIPGVEYDPEIGIFGMDVCVTLERPGFRVAKRKRQRRKIPTK
HKLTKEEGIVFAMEELKAKVEGLQ5JJG150S ribosomal protein L6Thermococcus kodakarensis0.0208882711LEVOMPVDAWIREEIEIPEGVEVTVEGNTVKVKGPKGELQRELKYPGVQIFTEDGKVVIYKEFPRKKDVAIVRTFKAHINNMIK
GVTEGFKYRLKVVYSHFPMTVKVQGDEVVIENFLGEKNPRRAKILPGVTVKVKGSEIEVEGIDKEAVGQTAANIEQATRI
TKWDRRVFQDGIYIVEKAGKPIKFQ5JJG450S ribosomal protein L7AeThermococcus kodakarensis0.0134546229999999992LEVOMAKPSYVKFEVPQELAEKALEAVEIARDTGRIRKGTNETTKAVERGQAKLVIIAEDVDPEEIVAHLPPLCEEKEIPYVYV
PSKKELGAAAGLEVPAASVAIIEPGKARELVEDIAMKVKELMKQ5JGR350S ribosomal protein L10eThermococcus kodakarensis0.0211165961LEVOMGLRPAKIDRDVDKPAYTRREYIRGAPGPKITIFDMGNLSAEFEYEVSLHAEQAMQIRQNALEAIRIQVNRYLQKNVGRS
NYHFKIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVRKDQKILTVWVNGQHLKFALGAMHRAKMKLPYSA
YYRIYDKEGNDVTTKVLSTMKLQ5JDI650S ribosomal protein L13Thermococcus kodakarensis0.0163161731LEVOMRIINAEGLILGRLASKVAKMLLEGEEVVIVNAEKAIITGNREDIFAKYKQRTELRTRTNPRRGPFYPKRSDEIVRRTVR
GMLPWKTDRGRKAFKRLKVYVGVPKEFEGKEFETISEAHMSRLATPKYVTVGEVAKFLGGKFQ5JJF650S ribosomal protein L14Thermococcus kodakarensis0.0151818881LEVOMAKKGAGATRGISPVRPTRALPIGAYLKVADNSGAKVIQIIGVVGYKGTRRRLAAAGVGDMVVATVKKGRPDMRHQVVRA
VVVRQRKEYRRLDGMRVKFEDNAAAIVTPEGVPRGTEIRGAIAREAAERWVRLGSIASIVLQ5JJF850S ribosomal protein L14eThermococcus kodakarensis0.0089685272LEVOMPAIEVGRIAVVIAGRRAGQKVVVADIIDKNFVLVTGAGLNKVKRRRMNVKHLEPLPERVNIQRGASDEEIKAALEGAGI
SLEQ5JJE450S ribosomal protein L15Thermococcus kodakarensis0.0165714360000000021LEVOMIRRRKKVRKLRGSHTHGWGCKKKHRGGGSKGGRGMAGTGKRKDQKWTWTIKYAPDRLGKRGFHRPKAVQYIPKVINLND
IDENFELFKDAGIIYEEDGKLVFDATALGVDKILGTGKLTRALVVKAYYVTPKAEEKIKAAGGEVLLAQ5JJH050S ribosomal protein L15eThermococcus kodakarensis0.0226078631LEVOMGMYKYIREAWKSPKKSYVGELLKKRMIQWRRDPVVKRIERPTRLDRARSLGYQAKQGYVVVRVRVRRGGRKRPRWKGGR
KPSKMGMVKYSPKKSLQWIAEEKAARKFPNLEVLNSYWVGEDGMYKWFEVIMVDPHHPVIKSDPKIAWIAGKAHKGRVFR
GLTSAGKRSRGLLNKGKGAEKVRPSIRAHQGKGKQ5JH4350S ribosomal protein L18Thermococcus kodakarensis0.0228954491LEVOMAHGPRYRVPFRRRREGKTNYHKRLKLLKSKKPRLVVRKTLNHHIAQIVVYDPKGDKTLVSAHTMELMRDFGWKGHGGNT
PSAYLLGLLIGYKAKQAGIEEAILDIGLHPPTRGSSIFAVLKGAVDAGLNVPHSEEIYPEDYRINGEHIANYAKALKEED
EALYRKQFSGYLVKGLEPEKLPEHFEEVKAKIIEKFEGAREQ5JJG750S ribosomal protein L18eThermococcus kodakarensis0.0137991519999999991LEVOMVKRTGPTDINLRRLIRTLRKKSNEEGVKIWKDIAWRLERPRRQRAEVNISKINRYTKEGDVVIVPGSVLGAGRLEHKVV
VAAWKFSEAARRKIIEAGGEAISIEELMERNPKGSGVIIMEQ5JJF550S ribosomal protein L19eThermococcus kodakarensis0.0176671761LEVOMIMLKTQRRIAAELLKCGENRIWIDPERIEDVAAAITREDIKRLIHDGVIKKKPIKGQSRARARAFQEARKKGRHRGPGS
KKGKKTARMGKKEVWMMTIRALRKELRKLKAEGKLDAHTYRRLYIRAKGGQFKNKRQLYMFMQEHGILKEQ5JJG650S ribosomal protein L18AeThermococcus kodakarensis0.0092277081LEVOMEVKVFRVKGTFERLGKKQPFTKEYRALKEEHVRELVYSDIGSKHRVPRTKIWIESIEEIKPEEAEDPVVRRLSLELQ5JGT350S ribosomal protein L21eThermococcus kodakarensis0.0112903131LEVOMVKKAHSFRRKTRGKLSKHPRRRGLPPLTRFLQEFEVGQKVHIVIEPSYHKGMPDPRFHGRTGTVVGKRGDAYIVQITDG
GKVKTFFIHPVHLRPQKGQ5JI5150S ribosomal protein L22Thermococcus kodakarensis0.0178387769999999971LEVOMSRGRFSYSFQNFDPERMARASGRDLRISPKHSVELLREIRGMMLNDALRYLDDVIAKKRPVPMKRFNDSQGHKPGKGFG
PGRYPVKVAKAVKKILLNAKNNAEQKGLDVDRLKIIHAAAHRGPVLRGYIPRAFGRATPFNEQTTHIEIVVEEIRRQ5JDH450S ribosomal protein L23Thermococcus kodakarensis0.0099157691LEVOMDPYKVIIRPLVTEKAVSLIERENKLTFIVDRRATKQDIKRAVEEMFNVKVAKVNTLVTMKGEKKAYVKLKPEYDASEIA
ARLGLFQ5JDH150S ribosomal protein L24Thermococcus kodakarensis0.0142258089999999991LEVOMKLDMKQPRKQRKFLYNAPLHLRGKIMSAPLSKELREKYGVRNLPIRVGDKVKVMRGDFKGVEGKVVEVDLRRYRIHVEG
VTHKKTDGTEVFYPLHPSNVMIVELNLEDEKREKIIERRAAQ5JJF950S ribosomal protein L24eThermococcus kodakarensis0.0081115529999999991LEVOMARWNVCSYCGREFEPGTGKMFVRNDGRVLFFCSSKCEKYYFMGRNPRKLKWTKAFQEARLQRAKRKQ5JGR550S ribosomal protein L29Thermococcus kodakarensis0.0079375389999999991LEVOMKPSEIREMSIEEIDKKIRELRLELAKERGVLTMGASMENPMVIRNLRRDIARLLTIKREKLREKRQ5JDH650S ribosomal protein L30Thermococcus kodakarensis0.0178561931LEVOMAKLALIRLRSGIRARGEVRDTLAMLRLHRINHLVIVDDTPSYRGMIQKVKDYITWGEIDKETLAKLLRKRGRLIGNKPI
TDEYVKEKLGMTIEEFAEKVVNGEMKLRDLPNIKPVFRLHPPRGGLKGSKKRSFKEGGALGYRGEKINELIERMLQ5JJG950S ribosomal protein L30eThermococcus kodakarensis0.010972821LEVOMVDFAFELRKVQDTGKIVMGAKKSIHLAKVGGAKLIIVARNARPDIKEDIYYYAKLSGIPVYEFEGTSVELGTLLGRPHT
VSALAVIDPGESKILALAGGKEQ5JE3550S ribosomal protein L31eThermococcus kodakarensis0.0103542929999999991LEVOMPIKPGEEVIFVVPINKIKKRVPRWKRAPRAARFVREWIARHAKADEVIIGTDVNEKIWERGAEKPPGKLRVKVVVEESE
GKRIARVSLAQ5JGT550S ribosomal protein L32eThermococcus kodakarensis0.014654481LEVOMNEKARLLRIRAKLKRKKPRFLRQEWWRYPKFKNDPKWRRPKGIDSKMRLKKKGKPRSPSIGWSSPKAVRGLHPSGYEEV
LVHNVKELEAIDPTRQAARIAGTVGARKREMILARAKELGVKVLNQ5JJG550S ribosomal protein L34eThermococcus kodakarensis0.0105587829999999991LEVOMKPMYRSRSWRRKYVRTPGGRTVIHFERRKPKVAHCAMCGRPLNGVPRGRPSELRKLPKTKKRPERPYPNLCPSCMRKVM
KAQVRASITAQ5JJF7Ribosomal protein eL35AThermococcus kodakarensis0.0110648851LEVOMNGTTPWPEAEGVVVVRKAVVLSYVGSHEHRDPNRVILKPLDVNDRGSAARLIGRKVVWRTPTGRKLVGKIVRTHGTRGE
VKAVFKPGLPGQALGDYVEIL50S ribosomal protein L37AeThermococcus kodakarensis0.009240041LEVOMGRTTKVGSAGRFGPRYGLKIRRRVAAVEARMKQKHVCPVCGRKAVRRISTGIWQCQKCGATFAGGAYLPTTPAGKVAKR
VTASKAQ5JDM650S ribosomal protein L37eThermococcus kodakarensis0.0074708751LEVOMGAGTAPKGKRNRTPTHIRCRRCGRRAFNVKKGYCAACGFGRSRRMRKYSWSHKWRKKRNLSYQ5JIE150S ribosomal protein L39eThermococcus kodakarensis0.0062626791LEVOMARNKPFAKKLRLAKAAKQNRRVPVWVIVKTNRKVITHPKRRHWRRTKLKEQ5JGT650S ribosomal protein L40eThermococcus kodakarensis0.00578197099999999951LEVOMARFPEAEARIFRKLVCMRCGATNPWGAKKCRKCGYKGLRPKAREPRGGGRQ5JJD3LSU ribosomal protein L41EThermococcus kodakarensis0.0050514091LEVOMKRRPRKWKKKGRMRWKWIKKRIRRLKRQRRKERGLIQ5JEV050S ribosomal protein L44eThermococcus kodakarensis0.0112044411LEVOMKYPKQIRTYCPFCKRHTIHKVEKVKKRPRSELSAGQRRFRRKLKGYTGFPRPNPAGREKPVKKLDLRFRCTVCGKAHTR
GQGFRVKKFELVEVQ5JE51Nucleic acid-binding protein, containing C2H2 zinc-fingerThermococcus kodakarensis0.00548557499999999951LEVOMAVLKAIKIEDRDGEILFRCPRCGMVFRSAKAYTRHVNKAHGHLFRKQ5JFH3Predicted exosome subunit, UPF0023 familyThermococcus kodakarensis0.0268731231LEVOMPISVDKAVIARLKTHGETFEILVDPYLARDFKEGKDVPIEEILATPYVFKDAHKGDKASEHEMEKIFGTSDPYEVAKII
LRKGEVQLTAEQRRQMLEDKKRYIATVIHRHAVDPRTGYPHPVDRILRAMEEAGVHIDIFKDAEAQVPQVIKAIRPILPL
KLEVKVIAVKIPGDYVGRAYGEVRKFGNIKREEWGSDGSWMFVIEIPGGVEEEFYEKLNALTKGEAITKLLERKGLQ5JIV0Unknown ribosomal proteinThermococcus kodakarensis0.0024346611LEVOAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAZINC ION6.5409e-0514ZNwater1.8015e-053556HOHsingleParticleparticle0.47.5Quantifoil R2/2COPPER200CARBONCONTINUOUS2.0ETHANE100277.15FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.029000.0FEI FALCON III (4k x 4k)COUNTING34.01C12.65FSC 0.143 CUT-OFFRELION3116585MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODRIGID BODY FITemd_10224_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480408.0408.0408.090.090.090.0XYZ-0.0390422680.117055394-0.000018496510.00357443930.850.850.85AUTHOR30S body masked unsharpenedemd_10224_additional_3.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480408.0408.0408.090.090.090.0XYZ-0.0358427130.10084916-0.00000008507140.00255340010.850.850.85AUTHOR30S head masked unsharpenedemd_10224_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480408.0408.0408.090.090.090.0XYZ-0.045982230.13453987-0.000059744170.00466177850.850.850.85AUTHOR50S masked unsharpenedemd_10224_additional_6.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480408.0408.0408.090.090.090.0XYZ-0.26558730.497094780.00026311130.0109134250.850.850.85AUTHOR50S masked postprocessemd_10224_additional_4.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
480
480480
0
00480480480408.0408.0408.090.090.090.0XYZ-0.12963420.254080180.000058848080.00297197980.850.850.85AUTHOR30S head masked postprocessemd_10224_additional_5.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)