2024-05-22 REL PDBe PDBe PDBe 2019-08-14 2019-08-28 2019-09-04 2024-05-22 Cryo-EM structure of rhinovirus-A89 Wald J Goessweiner-Mohr N RV-A89, HRV-B5, virus, capsid protein Wald J Pasin M Richter M Walther C Mathai N Kirchmair J Makarov VA Goessweiner-Mohr N Marlovits TC Zanella I Real-Hohn A Verdaguer N Blaas D Schmidtke M Cryo-EM structure of pleconaril-resistant rhinovirus-B5 complexed to the antiviral OBR-5-340 reveals unexpected binding site. Proc.Natl.Acad.Sci.USA US 116 19109 19115 2019 31462495 doi:10.1073/pnas.1904732116 1091-6490 0040 PNASA6 6sk7 FULLOVERLAP Rhinovirus A Rhinovirus A 0 1 2 3 4 Rhinovirus A A89 Homo sapiens VP1-4 302.0 VIRION SEROTYPE false false VP1 capsid protein Human rhinovirus A serotype 89 (strain 41467-Gallo) 0.033288102 1 LEVO NPVENYIDSVLNEVLVVPNIQPSTSVSSHAAPALDAAETGHTSSVQPEDMIETRYVITDQTRDETSIESFLGRSGCIAMI EFNTSSDKTEHDKIGKGFKTWKVSLQEMAQIRRKYELFTYTRFDSEITIVTAAAAQGNDSGHIVLQFMYVPPGAPVPEKR DDYTWQSGTNASVFWQEGQPYPRFTIPFMSIASAYYMFYDGYDGDSAASKYGSVVTNDMGTICVRIVTSNQKHDSNIVCR IYHKAKHIKAWCPRPPRAVAYQHTHSTNYIPSNGEATTQIKTRPDVFTVTNVGPSSMF P07210 3.4.22.29 VP2 capsid protein Human rhinovirus A serotype 89 (strain 41467-Gallo) 0.029422793 1 LEVO SPTVEACGYSDRLIQITRGDSTITSQDTANAVVAYGVWPSYLTPDDATAIDKPTQPDTSSNRFYTLDSRSWTSASSGWWW KLPDALKNMGIFGENMFYHFLGRSGYTIHVQCNSSKFHQGLLIVAAIPEHQLASATSGNVSVGYNHTHPGEQGREVVPSR TSSDNKRPSDDSWLNFDGTLLGNLPIYPHQYINLRTNNSATLILPYVNAVPMDSMLRHNNWSLVIIPICPLQVQPGGTQS IPITVSISPMFSEFSGPRSKVVFSTTQ P07210 3.4.22.29 VP3 capsid protein Human rhinovirus A serotype 89 (strain 41467-Gallo) 0.026325994 1 LEVO GLPVMLTPGSGQFLTTDDTQSPSAFPYFHPTKEIFIPGQVRNLIEMCQVDTLIPVNNTQENVRSVNMYTVDLRTQVDLAK EVFSIPVDIASQPLATTLIGELASYYTHWTGSLRFSFMFCGSASSTLKLLIAYTPPGVGKPKSRREAMLGTHLVWDVGLQ STASLVVPWVSASHFRFTTPDTYSSAGYITCWYQTNFVVPDSTPDNAKMVCMVSACKDFCLRLARDTNLHTQEGVLTQ P07210 3.4.22.29 VP4 capsid protein Human rhinovirus A serotype 89 (strain 41467-Gallo) 0.007461104 1 LEVO MGAQVSRQNVGTHSTQNSVSNGSSLNYFNINYFKDAASSGASRLDFSQDPSKFTDPVKDVLEKGIPTLQ D3KZ50 singleParticle particle 7.4 PBS 10.0 DMSO Homemade COPPER CARBON CONTINUOUS 0.4 GLOW DISCHARGE ETHANE 100 FEI VITROBOT MARK III FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 GATAN K2 SUMMIT (4k x 4k) COUNTING 1-25 5370 5.0 40.0 1 C60 2.9 FSC 0.143 CUT-OFF 107054 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_10222.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 -224 -224 -224 450 450 450 436.5 436.5 436.5 90.0 90.0 90.0 X Y Z -0.048386812 0.08026486 0.00041190247 0.004740334 0.97 0.97 0.97 0.02 AUTHOR 1AYM PDB experimental model
Rosetta: DiMaio, F. et al. Atomic-accuracy models from 4.5-A cryo-electron microscopy data with density-guided iterative local refinement. Nat. Meth (2015). doi:10.1038/nmeth.3286
REAL emd_10222_msk_1.map emd_10222_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 436.5 436.5 436.5 90.0 90.0 90.0 X Y Z -0.012084797 0.021146704 0.0000554948 0.0020261141 0.97 0.97 0.97 AUTHOR emd_10222_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 436.5 436.5 436.5 90.0 90.0 90.0 X Y Z -0.015071177 0.020723341 0.000054465454 0.0020260422 0.97 0.97 0.97 AUTHOR