REL PDBe PDBe PDBe 2003-02-10 2003-02-12 2003-02-12 2011-05-26 Three-dimensional reconstructions from cryoelectron microscopy images reveal an intimate complex between helicase DnaB and its loading partner DnaC. San Martin C San Martin C Radermacher M Wolpensinger B Engel A Miles CS Dixon NE Carazo JM Three-dimensional reconstructions from cryoelectron microscopy images reveal an intimate complex between helicase DnaB and its loading partner DnaC. STRUCTURE 6 501 509 1998 9562559 DnaB from E.coli DnaB from E.coli homohexamer 1 0.3 DnaB helicase Escherichia coli AN1459/pPS560 E. coli 0.3 6 homohexamer true AN1459/pPS560 pPS560 GO:0003678 IPR001198 singleParticle particle .04 7.6
50 mM Tris.HCl pH 7.6 2 mM DTT 5 mM MgCl2 200 mM NaCl 0.25 mM ADP
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: double side blotting device
samples were adsorbed onto carbon-coated molybdenum holey grids after 30s glow discharge, and vitrified by quick plunging in liquid ethane in a double-side blotting device FEI/PHILIPS EM420 FLOOD BEAM BRIGHT FIELD LAB6 100 2 1.5 2.0 60000.0 GATAN LIQUID NITROGEN 98 KODAK SO-163 FILM EIKONIX IEEE 488 27 10 8. Gatan626 45 C3 OTHER 34.5 OTHER Xmipp, SPIDER 1369 emd_1022.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 50 50 50 0 0 0 50 50 50 190 190 190 90 90 90 X Y Z -9.14113 11.271599999999999 0.0000960484 2.93696 3.8 3.8 3.8 6.98 DnaB
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