2019-11-06 REL PDBe PDBe PDBe 2019-08-12 2019-08-28 2019-08-28 2019-11-06 Subtomogram average of E. coli 70S ribosome from FISE tilt series Eisenstein F Danev R Pilhofer M Eisenstein F Danev R Pilhofer M Improved applicability and robustness of fast cryo-electron tomography data acquisition. J.Struct.Biol. US 208 107 114 2019 31425790 doi:10.1016/j.jsb.2019.08.006 1095-8657 0803 JSBIEM Eisenstein F Danev R Pilhofer M Improved applicability and robustness of fast cryo-electron tomography data acquisition. J.Struct.Biol. US 2019 31425790 doi:10.1016/j.jsb.2019.08.006 1095-8657 0803 JSBIEM Eisenstein F Danev R Pilhofer M Improved applicability and robustness of fast cryo-electron tomography data acquisition J.Struct.Biol. US 2019 doi:10.​1016/​j.​jsb.​2019.​08.​006 1095-8657 0803 JSBIEM EMD-10211 associated EM volume 70S ribosome 70S ribosome 0 Escherichia coli DH5[alpha] subtomogramAveraging particle 7.5 Quantifoil R2/2 COPPER 200 ETHANE 100
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN
single-tilt axis holder
GATAN K3 BIOQUANTUM (6k x 4k) 0.5 150.0
FISEtomo SerialEM script
 1 3 C1 9.0 FSC 0.5 CUT-OFF emClarity 1.3.9 9923 12 11251 EMD-6311 template matching emClarity 1.3.9
emClarity
 emClarity 1.3.9 4 emClarity 1.3.9 ANGULAR RECONSTITUTION emClarity 1.3.9 emd_10211.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 234 234 234 0 0 0 234 234 234 491.39996 491.39996 491.39996 90.0 90.0 90.0 X Y Z -6.6730123 15.664622 0.06573385 0.66045946 2.1 2.1 2.1 0.43 AUTHOR