2020-11-25 REL PDBe PDBe PDBe 2019-07-30 2019-11-20 2019-11-20 2020-11-25 Medical Research Council (United Kingdom) MC_UP_120117 United Kingdom Medical Research Council (United Kingdom) MC_U105184322 United Kingdom Structure of DPS determined at 100 keV Naydenova K McMullan G Peet MJ Lee Y Edwards PC Chen S Leahy E Henderson R Russo CJ Naydenova K McMullan G Peet MJ Lee Y Edwards PC Chen S Leahy E Scotcher S Henderson R Russo CJ CryoEM at 100 keV: a demonstration and prospects. Iucrj UK 6 1086 1098 2019 31709064 doi:10.1107/S2052252519012612 2052-2525 EMD-10161 associated EM volume DPS from E. coli DPS from E. coli 0 Escherichia coli Escherichia coli BL21(DE3) singleParticle particle 7.7 UltrAuFoil GOLD 300 ETHANE
FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 100 OTHER 24.64 1 T 3.4 FSC 0.143 CUT-OFF 16507 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_10161.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 188.16 188.16 188.16 90.0 90.0 90.0 X Y Z -0.23146866 0.350141 0.001044793 0.01685775 1.47 1.47 1.47 0.07 AUTHOR Masked and sharpened (B = -132 A^2) DPS map emd_10161_msk_1.map emd_10161_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 188.16 188.16 188.16 90.0 90.0 90.0 X Y Z -0.040616106 0.1186543 -0.00021730381 0.009181498 1.47 1.47 1.47 AUTHOR Unsharpened DPS map emd_10161_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 188.16 188.16 188.16 90.0 90.0 90.0 X Y Z -0.047944605 0.1295206 -0.00023786118 0.009633773 1.47 1.47 1.47 AUTHOR DPS half map 1 emd_10161_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 188.16 188.16 188.16 90.0 90.0 90.0 X Y Z -0.040968183 0.12410178 -0.00022818791 0.009543091 1.47 1.47 1.47 AUTHOR DPS half map 2