REL PDBe PDBe PDBe 2002-10-15 2002-10-15 2002-10-15 2013-02-27 Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. Stewart PL Fuller SD Burnett RM Stewart PL Fuller SD Burnett RM Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. EMBO J. 12 2589 2599 1993 8334984 adenovirus type 2 adenovirus type 2 T=25 particle 1 150 Human adenovirus 2 Human adenovirus 2 Homo sapiens VERTEBRATES fibres (5f positions) 1090A 25 capsid (along 2f) 884A 1 VIRION STRAIN false false singleParticle particle 10 7.4
Sample stored in CsCl was diluted in Tris (10mM) NaCl (100 mM) pH 7.4
 ETHANE 60 23 HOMEMADE PLUNGER
Vitrification instrument: EMBL plunger. plunging at ambient temperature and humidity
Blot for 2 sec FEI/PHILIPS EM400 FLOOD BEAM BRIGHT FIELD TUNGSTEN HAIRPIN 80 2 1.0 8.8 34000.0 GATAN LIQUID NITROGEN 105
Details of the microscopy are in Stewart, P. L., Burnett, R. M., Cyrklaff, M. and Fuller, S. D. (1991). Image reconstruction reveals the complex molecular organization of adenovirus. Cell 67, 145-54. Images were taken in triples at 1, 2.6 and 8.8 microns underfocus. Some images were taken at 12 degrees tilt to compensate for the preference for the virus to orient with the fibres away from the water layer. For image reconstruction see: Stewart, P. L., Burnett, R. M.,Cyrklaff, M. and Fuller, S. D. (1991). Image reconstruction reveals the complex molecular organization of adenovirus. Cell 67, 145-54. For reconstruction details see: P.L. Stewart, S.D. Fuller, R. M. Burnett, (1993). Difference imaging of adenovirus: bridging the resolution gap between X- ray crystallography and electron microscopy EMBO J.Stewart PL, Burnett RM. Adenovirus structure by X-ray crystallography and electron microscopy. Curr Top Microbiol Immunol. 1995, 199 (1):25-38. The PDB code for the Ad2 hexon model is 1DHX, and the reference Athappilly, F. K., Murali, R., Rux, J. J., Cai, Z., Burnett, R. M.: The refined crystal structure of hexon, the major coat protein of adenovirus type 2, at 2.9 A resolution. J Mol Biol 242 pp. 430 (1994)
1990-01-01 KODAK SO-163 FILM OPTRONICS 25 50 8 1.0 8. eucentric - Philips EM400 12
Purified by centrifugation in CsCl
initially different defocuses were combined by summing in resolution bands
 I OTHER 25.0 OTHER EMBL-ICOS 29
sufficient to give maximum inverse eigenvalue of 0.1
 emd_1016.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 161 161 161 0 0 0 161 161 161 1175.3 1175.3 1175.3 90.0 90.0 90.0 X Y Z 0.0 255.0 112.588562010000004 18.983009339999999 7.3 7.3 7.3 148.0 AUTHOR Adeno-virus type 2.
::::EMDATABANK.org::::EMD-1016::::
 RIGID BODY FIT maximizing cross correlation
Protocol: rigid body
max cross correlation REAL 3000.0
emd_1016.tif
emd_1016_1_slc.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 161 161 1 -80 -80 0 161 161 0 161.000 161.000 1.000 90.000 90.000 90.000 X Y Z 9.0 233.0 117.441299999999998 0.0 1 1 1
Created by IMAGIC: MRC image = /ebi/msd/emdeposit/submissions/EMD-1251/UP/tempCC P4/m 15-10-20 14:45:12
 emd_1016_2_slc.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 161 161 1 -80 -80 0 161 161 0 161.000 161.000 1.000 90.000 90.000 90.000 X Y Z 8.0 191.0 112.53501 0.0 1 1 1
Created by IMAGIC: MRC image = /ebi/msd/emdeposit/submissions/EMD-1251/UP/tempCC P4/m 15-10-20 14:46:37
 emd_1016_3_slc.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 161 161 1 -80 -80 0 161 161 0 161.000 161.000 1.000 90.000 90.000 90.000 X Y Z 0.0 239.0 115.471819999999994 0.0 1 1 1 map
Created by IMAGIC