2020-11-25 REL PDBe PDBe PDBe 2019-07-18 2019-10-23 2019-10-23 2020-11-25 Medical Research Council (United Kingdom) MR/R000352/1 United Kingdom Wellcome Trust 079605/Z/06/Z United Kingdom Medical Research Council (United Kingdom) MR/J003867/1 United Kingdom Biotechnology and Biological Sciences Research Council BB/L014211/1 United Kingdom Wellcome Trust 101488/Z/13/Z United Kingdom MKLP2-decocrated 13 protofilament microtubule (ADP.Al.Fx state) processed with MiRP (Microtubule Relion-based Pipeline) Cook AC Manka SW Wang S Moores CA Atherton J Cook AD Manka SW Wang S Moores CA Atherton J A microtubule RELION-based pipeline for cryo-EM image processing. J.Struct.Biol. US 209 107402 107402 2020 31610239 doi:10.1016/j.jsb.2019.10.004 1095-8657 0803 JSBIEM Atherton J Yu IM Cook A Muretta JM Joseph A Major J Sourigues Y Clause J Topf M Rosenfeld SS Houdusse A Moores CA The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry. Elife US 28826477 2050-084X EMD-3622 other EM volume
Data originally processed with Spider and Frealign
 EMD-10131 associated EM volume Complex of 13pf microtubule with bound MKLP2 motor domain in the presence of ADP.AlFx Complex of 13pf microtubule with bound MKLP2 motor domain in the presence of ADP.AlFx 0 1 2 3
Microtubule bound to taxol
 Tubulin alpha and beta chains 1 2 3
Microtubule bound to taxol
Bos taurus Kinesin-like protein KIF20A 1 1
Microtubule bound to taxol
Mus musculus Escherichia coli singleParticle filament 6.8 ETHANE
FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 DIRECT ELECTRON DE-20 (5k x 3k) INTEGRATING 25.0 1 Gctf
RELION
 4.2 FSC 0.143 CUT-OFF RELION
Following the MiRP pipeline
14411 PROJECTION MATCHING RELION
Following the MiRP pipeline
 PROJECTION MATCHING RELION
Following the MiRP pipeline
 RELION
Following the MiRP pipeline
 emd_10131.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 80 103 109 192 83 157 80 103 109 122.72 158.002 167.20601 90.0 90.0 90.0 X Y Z -0.15499362 0.29427066 0.001958117 0.01719634 1.534 1.534 1.534 0.035 AUTHOR MKLP2 (ADPAlFx state) decorated 13PF microtubule: symmetrised asymmetric unit density from MiRP processing 5ND4 emd_10131_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 662.688 662.688 662.688 90.0 90.0 90.0 X Y Z -5.131995 10.125138 0.013429667 0.72275335 1.534 1.534 1.534 AUTHOR MKLP2 (ADPAlFx state) decorated 13PF microtubule: C1 whole microtubule density from MiRP processing