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        <key_dates>
            <deposition>2019-06-25</deposition>
            <header_release>2019-07-17</header_release>
            <map_release>2020-02-12</map_release>
            <update>2025-07-02</update>
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        <title>KimA from Bacillus subtilis in inward-facing, occluded state</title>
        <authors_list>
            <author>Tascon I</author>
            <author>Sousa JS</author>
        </authors_list>
        <keywords>potassium transporter, LeuT fold, symporter, SMALP, TRANSPORT PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-2526-6238" order="1">Tascon I</author>
                    <author order="2">Sousa JS</author>
                    <author order="3">Corey RA</author>
                    <author order="4">Mills DJ</author>
                    <author ORCID="0000-0002-3564-897X" order="5">Griwatz D</author>
                    <author order="6">Aumuller N</author>
                    <author order="7">Mikusevic V</author>
                    <author ORCID="0000-0001-8800-7669" order="8">Stansfeld PJ</author>
                    <author order="9">Vonck J</author>
                    <author ORCID="0000-0003-1495-3163" order="10">Hanelt I</author>
                    <title>Structural basis of proton-coupled potassium transport in the KUP family.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>11</volume>
                    <first_page>626</first_page>
                    <last_page>626</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">32005818</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-020-14441-7</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <pdb_id>6s3k</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
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            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>KimA</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>KimA</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>APC family permease</name>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.066838977</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MYHSIKRFLIGKPLKSQAAGEQKLTKLKALAMLSSDALSSVAYGTEQILIILATISAAAFWYSIPIAVGVLILLLALILS
YRQIIYAYPQGGGAYIVSKENLGEKPGLIAGGSLLVDYILTVAVSISAGTDAITSAFPALHDYHVPIAIFLVLVIMILNL
RGLSESASILAYPVYLFVVALLVLIAVGLFKLMTGQIDQPAHHTSLGTPVAGITLFLLLKAFSSGCSALTGVEAISNAIP
AFKNPPARNAARTLAMMGILLAILFSGITVLAYGYGTAPKPDETVVSQIASETFGRNVFYYVIQGVTSLILVLAANTGFS
AFPQLAFNLARDQYMPRMFTVRGDRLGFSNGIIFLGFASIVLIILFGGQTEHLIPLYAVGVFIPFTLSQTGMCMKWIKQK
PKGWIGKMLINSCGALISFMVLSILFVTKFNVVWPVLIFMPIVVLLFFAIKNHYTAVGEQLRIVDKEPEEIKGTVVIVPV
AGVTTVVQKSIHYAKSLSDQVIAVHVSFDREQEKKFEKRWEELNNGVRLVTLHSSYRSLVHPFDKFLETVEAKAKKEQFS
VMVLFPQFITKKRWHTILHNQSAFLLRVRLFWKKDIMVATLPYHFKK</string>
                    <external_references type="UNIPROTKB">A0A164U4X9</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="2">
                <name>POTASSIUM ION</name>
                <molecular_weight>
                    <theoretical units="MDa">3.9098e-05</theoretical>
                </molecular_weight>
                <number_of_copies>6</number_of_copies>
                <formula>K</formula>
            </ligand>
        </macromolecule_list>
    </sample>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>8.0</ph>
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                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
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                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
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                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">1.77</average_electron_dose_per_image>
                        </image_recording>
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            </microscopy_list>
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                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
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