2024-10-16 REL PDBe PDBe PDBe 2019-06-18 2019-08-21 2019-08-21 2024-10-16 European Research Council 322581 Danish National Research Foundation Denmark Erythromycin Resistant Staphylococcus aureus 50S ribosome (delta R88 A89 uL22) in complex with erythromycin. Halfon Y Matozv D Ribosome, antibiotics, resistance, Staphylococcus aureus, exit tunnel, RNA, rProteins, erythromycin Halfon Y Matzov D Eyal Z Bashan A Zimmerman E Kjeldgaard J Ingmer H Yonath A Exit tunnel modulation as resistance mechanism of S. aureus erythromycin resistant mutant. Sci Rep UK 9 11460 11460 2019 31391518 doi:10.1038/s41598-019-48019-1 2045-2322 6s0z FULLOVERLAP Erythromycin Resistant Staphylococcus aureus 70S ribosome (delta R88 A89 uL22) in complex with erythromycin. Erythromycin Resistant Staphylococcus aureus 70S ribosome (delta R88 A89 uL22) in complex with erythromycin. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Staphylococcus aureus 23S ribosomal RNA Staphylococcus aureus 0.9409759999999999 1 UUAAGUUAUUAAGGGCGCACGGUGGAUGCCUUGGCACUAGAAGCCGAUGAAGGACGUUACUAACGACGAUAUGCUUUGGG GAGCUGUAAGUAAGCUUUGAUCCAGAGAUUUCCGAAUGGGGAAACCCAGCAUGAGUUAUGUCAUGUUAUCGAUAUGUGAA UACAUAGCAUAUCAGAAGGCACACCCGGAGAACUGAAACAUCUUAGUACCCGGAGGAAGAGAAAGAAAAUUCGAUUCCCU UAGUAGCGGCGAGCGAAAUGGGAAGAGCCCAAACCAACAAGCUUGCUUGUUGGGGUUGUAGGACACUCUACGGAGUUACA AAGGACGACAUUAGACGAAUCAUCUGGAAAGAUGAAUCAAAGAAGGUAAUAAUCCUGUAGUCGAAAAUGUUGUCUCUCUU GAGUGGAUCCUGAGUACGACGGAGCACGUGAAAUUCCGUCGGAAUCUGGGAGGACCAUCUCCUAAGGCUAAAUACUCUCU AGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAUAGAACCUGAAACCGU GUGCUUACAAGUAGUCAGAGCCCGUUAAUGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUUGAUGC AAGGUUAAGCAGUAAAUGUGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGUUUAGUAUUUGGUCGUAGACCCGAAACC AGGUGAUCUACCCUUGGUCAGGUUGAAGUUCAGGUAACACUGAAUGGAGGACCGAACCGACUUACGUUGAAAAGUGAGCG GAUGAACUGAGGGUAGCGGAGAAAUUCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCU CAAGUGAUGAUUAUUGGAGGUAGAGCACUGUUUGGACGAGGGGCCGGUUACCGAAUUCAGACAAACUCCGAAUGCCAAUU AAUUUAACUUGGGAGUCAGAACAUGGGUGAUAAGGUCCGUGUUCGAAAGGGAAACAGCCCAGACCACCAGCUAAGGUCCC AAAAUAUAUGUUAAGUGGAAAAGGAUGUGGCGUUGCCCAGACAACUAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAG AGUGCGUAAUAGCUCACUAGUCGAGUGACACUGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAGCUGUGGAUUG UCCUUUGGACAAUGGUAGGAGAGCGUUCUAAGGGCGUUGAAGCAUGAUCGUAAGGACAUGUGGAGCGCUUAGAAGUGAGA AUGCCGGUGUGAGUAGCGAAAGACGGGUGAGAAUCCCGUCCACCGAUUGACUAAGGUUUCCAGAGGAAGGCUCGUCCGCU CUGGGUUAGUCGGGUCCUAAGCUGAGGCCGACAGGCGUAGGCGAUGGAUAACAGGUUGAUAUUCCUGUACCACCUAUAAU CGUUUUAAUCGAUGGGGGGACGCAGUAGGAUAGGCGAAGCGUGCGAUUGGAUUGCACGUCUAAGCAGUAAGGCUGAGUAU UAGGCAAAUCCGGUACUCGUUAAGGCUGAGCUGUGAUGGGGAGAAGACAUUGUGUCUUCGAGUCGUUGAUUUCACACUGC CGAGAAAAGCCUCUAGAUAGAAAAUAGGUGCCCGUACCGCAAACCGACACAGGUAGUCAAGAUGAGAAUUCUAAGGUGAG CGAGCGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUCUUUAGGGUUAACGCCCA GAAGAGCCGCAGUGAAUAGGCCCAAGCGACUGUUUAUCAAAAACACAGGUCUCUGCUAAACCGUAAGGUGAUGUAUAGGG GCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGGUUAGCUUCUGCGAAGCUACGAAUCGAAGCCCCAGUAAACGG CGGCCGUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGUAACGAUUUGGGC ACUGUCUCAACGAGAGACUCGGUGAAAUCAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGU GGAGCUUUACUGUAGCCUGAUAUUGAAAUUCGGCACAGCUUGUACAGGAUAGGUAGGAGCCUUUGAAACGUGAGCGCUAG CUUACGUGGAGGCGCUGGUGGGAUACUACCCUAGCUGUGUUGGCUUUCUAACCCGCACCACUUAUCGUGGUGGGAGACAG UGUCAGGCGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCUCAAAGGUUCCCUCAGAAUGGUUGG AAAUCAUUCAUAGAGUGUAAAGGCAUAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGGGUCGAAAGACGGACUUA GUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCA AGAGUUCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUG GGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGUGGGCGU AGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACAUACCUCUGGUGUACCAGUUGUCGUGCCAACG GCAUAGCUGGGUAGCUAUGUGUGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCC CAACUUCGGUUAUAAGAUCCCUCAAAGAUGAUGAGGUUAAUAGGUUCGAGGUGGAAGCAUGGUGACAUGUGGAGCUGACG AAUACUAAUCGAUCGAAGACUUAAU 5S ribosomal RNA Staphylococcus aureus 0.036669766 1 UCUGGUGACUAUAGCAAGGAGGUCACACCUGUUCCCAUGCCGAACACAGAAGUUAAGCUCCUUAGCGUCGAUGGUAGUCG AACUUACGUUCCGCUAGAGUAGAACGUUGCCAGG 50S ribosomal protein L2 Staphylococcus aureus 0.029827607 1 LEVO AIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRNNQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSI QYDPNRSANIALVVYADGEKRYIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGAS AQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPI GRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRK B3VKN3 50S ribosomal protein L3 Staphylococcus aureus 0.023199545 1 LEVO TKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVDGYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKAD AAPKRFIREFRNVDVDAYEVGQEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDAS RVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIK W8U3W0 50S ribosomal protein L4 Staphylococcus aureus 0.022364504 1 LEVO ANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASLRQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAP QWRGGGIVFGPTPRSYAYKMPKKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENEDV NVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG W8TRC7 50S ribosomal protein L5 Staphylococcus aureus 0.019460596 1 LEVO LKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAVQNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGM PIGAKVTLRGERMYEFLDKLISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTANTD EEARELLANFGMP W8TUC3 50S ribosomal protein L6 Staphylococcus aureus 0.019270869 1 LEVO SRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFKQEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQ GYVKVLELVGVGYRAQMQGKDLILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGKG IRYQGEYVRRKEG W8U3X2 50S ribosomal protein L13 Staphylococcus aureus 0.016359427 1 LEVO MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVTYTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSN HPGGIKSITAGELRRTNPERLIENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG W8TUE6 50S ribosomal protein L14 Staphylococcus aureus 0.013157342 1 LEVO MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVKNATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFD ENACVIIRDDKGPRGTRIFGPVARELREGNFMKIVSLAPEVL A0A077UUA0 50S ribosomal protein L15 Staphylococcus aureus 0.01562889 1 LEVO MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGGGVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLD QLNKFEDGTEVTPALLVESGVVKNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI A0A077UGA7 50S ribosomal protein L16 Staphylococcus aureus 0.015599434999999998 1 LEVO MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITSRQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLE VRMGAGKGAVEGWIAVVKPGRILFEVAGVSEEVAREALRLASHKLPVKTKFVKREEL A0A077V4G0 50S ribosomal protein L17 Staphylococcus aureus 0.013583522999999998 1 LEVO YRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVVEKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTAL QKLFGEIAERYTERQGGYTRILKQGPRRGDGAESVIIELV A0A077UG91 50S ribosomal protein L18 Staphylococcus aureus 0.012992897 1 LEVO ISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQIIDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIA KKAADKGIKEIVFDRGGYLYHGRVKALAEAARESGLEF W8TRE0 50S ribosomal protein L19 Staphylococcus aureus 0.013104380999999998 1 LEVO TNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQVFEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTP KIEKIEVKRRGKVRRAKLYYLRSLRGKAARIQEI A0A077UVB6 50S ribosomal protein L20 Staphylococcus aureus 0.013459918999999999 1 LEVO PRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQYAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMN GLKKAGIDINRKMLSEIAISDEKAFAQLVTKAKDAL A0A077VMP6 50S ribosomal protein L21 Staphylococcus aureus 0.011354081 1 LEVO MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGDSVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRK NSKRKKGHRQPYTKLTIDKINA D7URR3 50S ribosomal protein L22 Staphylococcus aureus 0.012100086999999999 1 LEVO MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASSPVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGP TLKRFRPQGRASAINKRTSHITIVVSDGKE A0A077UKF9 50S ribosomal protein L23 Staphylococcus aureus 0.0103801 1 LEVO EARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEEIFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVT LKEGSIDLF W8TUB4 50S ribosomal protein L24 Staphylococcus aureus 0.011316205 1 LEVO HIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKHQKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRV GYKFVDGKKVRIAKKSGEEIKSN W8TRD5 50S ribosomal protein L25 Staphylococcus aureus 0.0104622 1 LEVO ASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVDEVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDP LKNQITHIDFLAIN A0A133Q8Z9 50S ribosomal protein L27 Staphylococcus aureus 0.008409477999999998 1 LEVO STKNGRDSESKRLGAKRADGQFVTGGSILYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVA A0A077ULC5 50S ribosomal protein L28 Staphylococcus aureus 0.005499431999999999 1 LEVO ASTGNRRSHALNSTKRRWNANLQKVRILVDGKPKKVWVSARALKSGKVT A0A077URJ8 50S ribosomal protein L29 Staphylococcus aureus 0.007845942 1 LEVO KAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIRTVRKTIARLKTVAREREIEQSKAN A0A077W1J5 50S ribosomal protein L30 Staphylococcus aureus 0.006434487 1 LEVO AKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIRGQINKVKHLVTVEEK W8UVN7 50S ribosomal protein L31 type B Staphylococcus aureus 0.006909694 1 LEVO MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYPVIRLDISSDSHPFYT A0A380EL30 50S ribosomal protein L32 Staphylococcus aureus 0.005569564999999999 1 LEVO AVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCKNCGSY A0A1Q8DAT0 50S ribosomal protein L33 Staphylococcus aureus 0.0056845609999999994 1 LEVO RVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTLHRET A0A077V2P0 50S ribosomal protein L34 Staphylococcus aureus 0.005252369 1 LEVO VKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS W8TQ59 50S ribosomal protein L35 Staphylococcus aureus 0.007461992 1 LEVO PKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQLRKARLVSKSDMKRVKQLLAYK A0A077UL47 50S ribosomal protein L36 Staphylococcus aureus 0.004318422 1 LEVO MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG A0A077UGV8 ERYTHROMYCIN A 0.000733927 1 ERY singleParticle particle 7.6 ETHANE 100 277.15 FEI VITROBOT MARK IV FEI TITAN KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 1.076 1 5NGM C1 2.3 FSC 0.143 CUT-OFF RELION 3.0 378309 ANGULAR RECONSTITUTION RANDOM ASSIGNMENT emd_10077.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 426.80002 426.80002 426.80002 90.0 90.0 90.0 X Y Z -0.12392809 0.29235694 0.00021022212 0.0051371767 1.067 1.067 1.067 0.015 AUTHOR