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    <admin>
        <current_status>
            <date>2020-02-05</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-06-07</deposition>
            <header_release>2020-02-05</header_release>
            <map_release>2020-02-05</map_release>
            <update>2020-02-05</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Wellcome Trust</funding_body>
                <code>206422/Z/17/Z</code>
                <country>United Kingdom</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Biotechnology and Biological Sciences Research Council</funding_body>
                <code>BB/S003339/1</code>
                <country>United Kingdom</country>
            </grant_reference>
        </grant_support>
        <title>Structure of the E. coli Chemotaxis Core Signaling Unit</title>
        <authors_list>
            <author>Zhang P</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Cassidy CK</author>
                    <author order="2">Himes BA</author>
                    <author order="3">Sun D</author>
                    <author order="4">Ma J</author>
                    <author order="5">Zhao G</author>
                    <author order="6">Parkinson JS</author>
                    <author order="7">Stansfeld PJ</author>
                    <author order="8">Luthey-Schulten Z</author>
                    <author order="9">Zhang P</author>
                    <title>Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations.</title>
                    <journal_abbreviation>Commun Biol</journal_abbreviation>
                    <country>UK</country>
                    <volume>3</volume>
                    <first_page>24</first_page>
                    <last_page>24</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">31925330</external_references>
                    <external_references type="DOI">doi:10.1038/s42003-019-0748-0</external_references>
                    <external_references type="ISSN">2399-3642</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-10050</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>Bacterial chemotaxis core signaling complex</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Bacterial chemotaxis core signaling complex</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="562">Escherichia coli</organism>
                    <strain>K12</strain>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>subtomogramAveraging</method>
            <aggregation_state>twoDArray</aggregation_state>
            <specimen_preparation_list>
                <subtomogram_averaging_preparation preparation_id="1">
                    <buffer>
                        <ph>7.4</ph>
                    </buffer>
                    <grid>
                        <model>Quantifoil R2/2</model>
                        <material>COPPER</material>
                        <mesh>300</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY ARRAY</film_topology>
                        </support_film>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">90</chamber_humidity>
                        <chamber_temperature units="K">298</chamber_temperature>
                        <instrument>HOMEMADE PLUNGER</instrument>
                        <details></details>
                    </vitrification>
                    <details>in vitro reconstitution of E coli chemosensory array of core signaling complexes</details>
                </subtomogram_averaging_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <subtomogram_averaging_microscopy microscopy_id="1">
                    <microscope>FEI POLARA 300</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">70.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.0</nominal_cs>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN ULTRASCAN 4000 (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">1.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </subtomogram_averaging_microscopy>
            </microscopy_list>
            <subtomogram_averaging_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C2</point_group>
                    </applied_symmetry>
                    <algorithm>BACK PROJECTION</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">8.4</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>emClarity</name>
                        </software>
                    </software_list>
                    <number_subtomograms_used>91636</number_subtomograms_used>
                </final_reconstruction>
                <extraction>
                    <number_tomograms>24</number_tomograms>
                    <number_images_used>91636</number_images_used>
                    <method>emClarity</method>
                    <software_list>
                        <software>
                            <name>emClarity</name>
                        </software>
                    </software_list>
                </extraction>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>emClarity</name>
                        </software>
                    </software_list>
                </ctf_correction>
                <final_angle_assignment>
                    <type>OTHER</type>
                    <software_list>
                        <software>
                            <name>emClarity</name>
                        </software>
                    </software_list>
                </final_angle_assignment>
            </subtomogram_averaging_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="33959">
        <file>emd_10050.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>204</col>
            <row>204</row>
            <sec>204</sec>
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            <col>0</col>
            <row>0</row>
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        <spacing>
            <x>204</x>
            <y>204</y>
            <z>204</z>
        </spacing>
        <cell>
            <a units="Å">627.50397</a>
            <b units="Å">627.50397</b>
            <c units="Å">627.50397</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-7.622192</minimum>
            <maximum>14.402969</maximum>
            <average>0.004676108</average>
            <std>0.17460589</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">3.0759997</x>
            <y units="Å">3.0759997</y>
            <z units="Å">3.0759997</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>2.15</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-10050::::</label>
        <annotation_details>E coli chemotaxis core signaling unit, cerrying receptor QQQQ mutations in the receptor.</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>FLEXIBLE FIT</refinement_protocol>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
    </interpretation>
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