REL PDBe PDBe PDBe 2002-08-28 2002-08-30 2003-08-30 2014-10-22 Structure of the Escherichia coli ribosomal termination complex with release factor 2. Klaholz BP Pape T Zavialov AV Myasnikov AG Orlova EV Vestergaard B Ehrenberg M van Heel M Klaholz BP Pape T Zavialov AV Myasnikov AG Orlova EV Vestergaard B Ehrenberg M van Heel M Structure of the Escherichia coli ribosomal termination complex with release factor 2. NATURE 421 90 94 2003 12511961 doi:10.1038/nature01225 1ml5 FULLOVERLAP release factor RF2 bound to E. coli ribosomes release factor RF2 bound to E. coli ribosomes monomer 2 2.3 sedimentation ribosome
Proteins L7/L12 of the LSU 50S subunit are not seen in the map
false Escherichia coli 2.3 2.3 ribosome-prokaryote: LSU 50S release factor 2 Escherichia coli BL21(DE3) BL21 (DE3) 0.040 0.04 1 monomer true Escherichia coli pET11a IPR004374 singleParticle particle 0.15 7.5
polymix buffer
 NEGATIVE
no staining, cryo-EM with holey carbon grids
 ETHANE 45 20 HOMEMADE PLUNGER
Vitrification instrument: home-made cryo-plunger
blot for 2 seconds before plunging FEI/PHILIPS CM200FEG/ST FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.1 0.5 2.9 50000.0 48000.0 GATAN LIQUID NITROGEN 100 102 100 2001-09-06 KODAK SO-163 FILM PATCHWORK DENSITOMETER 3 78 10 12. Side entry liquid nitrogen-cooled cryo specimen holder.
each particle
 C1 OTHER 14.0 OTHER IMAGIC
exact filtered back-projection
15800
beta gamma
 103 emd_1005.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 -60 -60 -60 120 120 120 290.28 290.28 290.28 90 90 90 X Y Z -0.159669 0.213811 -0.000130684 0.0224271 2.419 2.419 2.419 0.0401 E. coli ribosomal termination complex with release factor RF2.
::::EMDATABANK.org::::EMD-1005::::
 1GIX RIGID BODY FIT eye
Protocol: rigid body. rigid body of three individual domains keeping the connectivity; linkers betweenn domains defined based on temperature factors of crystal structure, conserved Gly and Pro residues, proteolytic sites, and global domain architecture
best visual fit using the program O REAL 1GIY RIGID BODY FIT eye
Protocol: rigid body. rigid body of three individual domains keeping the connectivity; linkers betweenn domains defined based on temperature factors of crystal structure, conserved Gly and Pro residues, proteolytic sites, and global domain architecture
best visual fit using the program O REAL