REL PDBe PDBe PDBe 2002-06-26 2002-08-22 2002-08-22 2012-10-17 Solution structure of the E. coli 70S ribosome at 11.5 A resolution. Gabashvili IS Gabashvili IS Agrawal RK Spahn CM Grassucci RA Svergun DI Frank J Penczek P Solution structure of the E. coli 70S ribosome at 11.5 A resolution. CELL(CAMBRIDGE,MASS.) 100 537 549 2000 10721991 doi:10.1016/S0092-8674(00)80690-X 1eg0 FULLOVERLAP FMet-tRNAMet 70S Ribosome from E.coli FMet-tRNAMet 70S Ribosome from E.coli
preparation and buffer conditions are as described in Malhotra et al., J. Mol. Biol. (1998) 280, 103-116
3 2.5 70S ribosome Escherichia coli false Escherichia coli ribosome-prokaryote: LSU 50S fMet-tRNA Escherichia coli OTHER DOUBLE HELIX false MF-mRNA Escherichia coli
46 nucleotides
OTHER SINGLE STRANDED false singleParticle particle 0.09 7.6
20 mM Hepes, 6 mM Mg(CH3COO)2 150 mM NH4Cl, 4 mM beta-Mercaptoethanol, 0.5 mM Spermine, 2 mM Spermidine.
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: 2 side blotting plunger
Blot and plunge FEI/PHILIPS CM200FEG/ST FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 2.17 4.34 50000.0 51200.0 GATAN LIQUID NITROGEN 93
25 % of data from Philips EM420 with LaB6 at 100kV
1997-10-04 KODAK SO-163 FILM PERKIN ELMER 15 239 10 12. Cryo Transfer 0 0
Wiener filtration of defocus group volumes
 C1 OTHER 11.5 FSC 0.5 CUT-OFF SPIDER/WEB 73523 emd_1003.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 125 125 125 -62 -62 -62 125 125 125 366.25 366.25 366.25 90 90 90 X Y Z -141.974999999999994 339.987000000000023 1.85585 38.046700000000001 2.93 2.93 2.93 82.799999999999997 E. coli 70S Ribosome
::::EMDATABANK.org::::EMD-1003::::
 RIGID BODY FIT O
manual fitting using O Mol_Id: 1; Molecule: S4 Ribosomal Protein; Chain: A; Other_Details: Modeled By Analogous Protein Of B. Stearothermophilus Taken From Pdb Entry 1C06 Mol_Id: 2; Molecule: S5 Ribosomal Protein; Chain: B; Other_Details: Modeled By Analogous Protein Of B. Stearothermophilus Taken From Pdb Entry 1Pkp Mol_Id: 3; Molecule: S6 Ribosomal Protein; Chain: C; Other_Details: Modeled By Analogous Protein Of T. Thermophilus Taken From Pdb Entry 1Ris Mol_Id: 4; Molecule: S7 Ribosomal Protein; Chain: D; Other_Details: Modeled By Analogous Protein Of T. Thermophilus Taken From Pdb Entry 1Rss Mol_Id: 5; Molecule: S8 Ribosomal Protein; Chain: E; Other_Details: Modeled By Analogous Protein Of T. Thermophilus Taken From Pdb Entry 1An7 Mol_Id: 6; Molecule: S15 Ribosomal Protein; Chain: F; Other_Details: Modeled By Analogous Protein Of B. Stearothermophilus Taken From Pdb Entry 1A32 Mol_Id: 7; Molecule: S17 Ribosomal Protein; Chain: G; Other_Details: Modeled By Analogous Protein Of B. Stearothermophilus Taken From Pdb Entries 1Rip and 1Qd7 Mol_Id: 8; Molecule: S20 Ribosomal Protein; Chain: H; Other_Details: Modeled By Analogous Protein Of T. Thermophilus Taken From Pdb Entry 1Qd7 Mol_Id: 9; Molecule: Ribosomal Protein L1; Chain: N; Other_Details: Modeled By Analogous Protein Of T. Thermophilus Taken From Pdb Entry 1Ad2 Mol_Id: 10; Molecule: Ribosomal Protein L6; Chain: J; Mutation: Yes; Other_Details: Modeled By Analogous Protein Of T. Stearothermophilus Taken From Pdb Entry 1Rl6 Mol_Id: 11; Molecule: Ribosomal Protein L11; Chain: K; Other_Details: Modeled By Analogous Protein Of T. Maritima Taken From Pdb Entry 1Mms Mol_Id: 12; Molecule: Fragment Of 16S Rrna Helix 23; Chain: I; Fragment: Residues 673-713; Other_Details: Modeled As Analogous Fragment Of T. Thermophilus Taken From Pdb Entry 1Qd7 Mol_Id: 13; Molecule: Fragment Of 23S Rrna; Chain: L; Fragment: Residues 1051-1108; Other_Details: T. Maritima RNA Sequence and Model Taken From Pdb Entry 1Mms Mol_Id: 14; Molecule: Helix 95 Of 23S Rrna; Chain: M; Other_Details: E. Coli RNA Sequence and Model Taken From Pdb Entry 480D Mol_Id: 15; Molecule: Formyl-Methionyl-tRNA; Chain: O; Synonym: Fmet-tRNA; Other_Details: E. Coli Fmet-tRNA Sequence and Model Taken From Pdb Entry 2Fmt