REL PDBe PDBe PDBe 2002-06-18 2002-06-20 2002-06-20 2012-12-26 Real space refinement of acto-myosin structures from sectioned muscle. Chen LF Blanc E Chapman MS Taylor KA Chen LF Blanc E Chapman MS Taylor KA Real space refinement of acto-myosin structures from sectioned muscle. J.STRUCT.BIOL. 133 221 232 2001 11472093 doi:10.1006/jsbi.2000.4321 1m8q FULLOVERLAP 1mvw FULLOVERLAP 1o18 FULLOVERLAP 1o19 FULLOVERLAP 1o1a FULLOVERLAP 1o1b FULLOVERLAP 1o1c FULLOVERLAP 1o1d FULLOVERLAP 1o1e FULLOVERLAP 1o1f FULLOVERLAP 1o1g FULLOVERLAP Rigor insect flight muscle from Lethocerus maximus Rigor insect flight muscle from Lethocerus maximus
The sample is a single filament layer cut from myofibrils by ultramicrotomy. The section thickness is ~25 nm. Specimen has been chemically fixed, dehydrated, embedded in Araldite and sectioned with a diamond knife.
Thick myosin containing filament and thin actin-containing filaments. 2 thick, myosin-containing filament
Filaments contain more than just myosin
false Lethocerus water bug, Lethocerus maximus Indirect flight muscles muscle fibers myofibrils myofibrils thin, actin-containing filament
filaments contain more than just actin
polar, 2-stranded helical filament, helical symmetry is 28/13 false Lethocerus water bug, Lethocerus maximus Indirect flight muscles Muscle fibers myofibrils myofibrils tomography NONE FEI/PHILIPS EM400 FLOOD BEAM BRIGHT FIELD TUNGSTEN HAIRPIN 100 17000.0 PHILIPS ROTATION HOLDER 25 25 25 PERKIN ELMER 25 24 16. Philips rotation-tilt holder 4.3 59.4 10
Double axis tilt series collected on film. Digitized on PDS 1010M densitometer. Other relevent references K. A. Taylor, M. C. Reedy, L. Cordova and M. K. Reedy. 3-D structure of insect flight muscle in rigor from tilted thin sections. Nature 310, 28 5-291 (1984). Hanspeter Winkler and Kenneth A. Taylor. Multivariate statistical analysis of three-dimensional cross-bridge motifs in insect flight muscle. Ultramicroscopy 77, 141-152 (1999). Chen, Li Fan, Winkler, Hanspeter, Reedy, Michael K., Ree dy, Mary C. and Taylor, Kenneth A.. Molecular Modeling of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle. J. Struct. Biol. 138(2), 92-104 (2002)
OTHER 40.0 OTHER
The reconstruction was done by aligning the members of the tilt series using crosscorrelation functions, such as phase only and the VanHeel Mutual Correlation Function. The sampling in each micrographof a tilted specimen was match by interpolation to th e lower angle members of the tilt series. This matching of areas and sampling was done by a 4 parameter grid search. Once aligned and scaled, the data were combined in Fourier space using a Whittaker-Shannon interpolation scheme. The map was calculated using a reverse Fourier transformation. The software used for the reconstruction was in-house and adapted from in some cases from MRC software.
30
none
 emd_1001.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 600 600 30 0 0 -15 600 600 30 9280 9280 464 90 90 90 X Y Z -417.992000000000019 366.680000000000007 1.85973 47.791600000000003 15.4667 15.4667 15.4667 81.200000000000003 Acto-myosin structures from sectioned muscle
::::EMDATABANK.org::::EMD-1001::::
 RIGID BODY FIT RSref
Protocol: real space rigid body. Domains were fit manually into the density using O. The entire light chain domain consisting of heavy chain residues from G710 to K843 and the two light chains were repositioned using G710 of the myosin heavy chain using O.Real space refinement done using 7 rigid bodies and 4 hinge points.
poor atom-atom contacts and cross correlation coefficient REAL emd_1001_msk_26.map emd_1001_msk_26.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.4923182 1.4168506 -0.0060140644 0.6964112 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:1:::
 emd_1001_msk_1.map emd_1001_msk_1.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5944648 1.1074616 -0.007541597 0.71632415 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:2:::
 emd_1001_msk_2.map emd_1001_msk_2.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.7258816 1.2866669 -0.0073102424 0.71558625 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:3:::
 emd_1001_msk_3.map emd_1001_msk_3.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5483093 1.2398646 -0.0034679337 0.71510464 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:4:::
 emd_1001_msk_4.map emd_1001_msk_4.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.501652 1.2972634 -0.008869538 0.7108043 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:5:::
 emd_1001_msk_5.map emd_1001_msk_5.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.4358373 1.3425884 -0.00060270604 0.69676816 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:6:::
 emd_1001_msk_6.map emd_1001_msk_6.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5323744 1.2563564 -0.0036051604 0.70039195 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:7:::
 emd_1001_msk_7.map emd_1001_msk_7.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.4987898 1.1380355 -0.005006318 0.7139458 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:8:::
 emd_1001_msk_8.map emd_1001_msk_8.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.6747184 1.0895122 -0.003031824 0.7237702 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:9:::
 emd_1001_msk_9.map emd_1001_msk_9.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.7482247 1.1234381 -0.0064628804 0.7249305 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:10:::
 emd_1001_msk_10.map emd_1001_msk_10.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5993469 1.1787673 -0.004972806 0.70850605 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:11:::
 emd_1001_msk_11.map emd_1001_msk_11.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.4103546 1.2010442 -0.004846287 0.7027782 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:12:::
 emd_1001_msk_12.map emd_1001_msk_12.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5464618 1.1851541 -0.0035916774 0.7222626 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:13:::
 emd_1001_msk_13.map emd_1001_msk_13.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.372035 1.1947367 -0.005957831 0.7072046 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:14:::
 emd_1001_msk_14.map emd_1001_msk_14.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.6208508 1.309575 -0.0046354607 0.7117156 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:15:::
 emd_1001_msk_15.map emd_1001_msk_15.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5245786 1.1403794 -0.004893605 0.72051364 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:16:::
 emd_1001_msk_16.map emd_1001_msk_16.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5550923 1.3233893 -0.0075495755 0.7237952 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:17:::
 emd_1001_msk_17.map emd_1001_msk_17.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.3360648 1.1792009 -0.004218508 0.68966633 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:18:::
 emd_1001_msk_18.map emd_1001_msk_18.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.6305237 1.5136678 0.0001082411 0.693829 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:19:::
 emd_1001_msk_19.map emd_1001_msk_19.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.7865362 1.6329293 -0.0028804576 0.7352475 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:20:::
 emd_1001_msk_20.map emd_1001_msk_20.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.443733 1.3822539 -0.0019926773 0.704275 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:21:::
 emd_1001_msk_21.map emd_1001_msk_21.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5086238 1.2634512 -0.0036103243 0.7252106 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:22:::
 emd_1001_msk_22.map emd_1001_msk_22.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5961685 1.1959407 0.0010213794 0.6944105 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:23:::
 emd_1001_msk_23.map emd_1001_msk_23.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.5973077 1.3023932 -0.0048314277 0.7077107 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:24:::
 emd_1001_msk_24.map emd_1001_msk_24.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z -2.3478413 1.1841714 -0.0023025929 0.70339763 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:25:::
 emd_1001_msk_25.map emd_1001_msk_25.map 1 IMAGE STORED AS SIGNED BYTE 32 30 36 0 0 0 32 30 36 464.0 550.0 622.0 90.0 90.0 90.0 X Y Z 0.0 1.0 0.047251157 0.21217561 15.466666 15.466666 15.466666 n/a
::::EMDATABANK.org::::EMD-1001::::MASK:26:::