2020-05-06 REL PDBe PDBe PDBe 2019-05-31 2019-06-26 2020-05-06 2020-05-06 National Research Foundation in South Africa 92556 South Africa Native encapsulin with bound dye decolourising peroxidase on three fold axis Kirykowicz AM Woodward JD Kirykowicz AM Woodward JD Structural pipeline for the investigation of Mycobacterial protein complexes involved in the stationary phase stress response To Be Published 0353 EMD-4175 other EM volume
Icosahedrally averaged encapsulin
 EMD-10004 other EM volume
Encapsulated dye decolourising peroxidase
 EMD-10005 other EM volume
Native bacterioferritin
 EMD-10008 other EM volume EMD-10008 associated EM volume Encapsulin with bound DyP on three fold axis Encapsulin with bound DyP on three fold axis 0 1
C3 symmetry applied to native encapsulin particles
Mycobacterium smegmatis str. MC2 155 Cytoplasm 1.962 singleParticle particle 0.3 8
200 mM NaCl, 50 mM Tris-HCl
 NEGATIVE Uranyl acetate
Sample washed/blotted with 2% UA and air-dried
Negative stain
 FEI TECNAI F20 OTHER OTHER FIELD EMISSION GUN 200 70.0 1.2 29000.0 GATAN ULTRASCAN 4000 (4k x 4k) 4000 4000 0.5 20.0 1 890
Manual picking
 EMD-4175
Low pass filtered to 40 angstrom
 C3 30.0 OTHER
Estimated from 3D spectra signal-to-noise ratio
890 PROJECTION MATCHING
Spider - matching against starting model
 PROJECTION MATCHING
50 iterations of 15 degrees
No classification
 emd_10008.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 -64 -64 -64 128 128 128 491.52 491.52 491.52 90.0 90.0 90.0 X Y Z -0.06991073 0.091429554 0.0003954494 0.011828498 3.84 3.84 3.84 0.0496 AUTHOR Encapsulin with Dyp on three fold axis