2024-05-22 REL PDBe PDBe PDBe 2019-05-24 2019-10-23 2019-10-23 2024-05-22 BACTERIOPHAGE SPP1 PROCAPSID-II PROTEIN Ignatiou A Brasiles S Bacteriophage, maturation process, cryo electron microscopy, capsid protein, 3D reconstruction, VIRUS Ignatiou A Brasiles S El Sadek Fadel M Burger J Mielke T Topf M Tavares P Orlova EV Structural transitions during the scaffolding-driven assembly of a viral capsid. Nat Commun UK 10 4840 4840 2019 31649265 doi:10.1038/s41467-019-12790-6 2041-1723 EMD-4717 other EM volume EMD-4716 other EM volume 6rtl FULLOVERLAP Bacillus phage SPP1 Bacillus phage SPP1 0 1 Bacillus phage SPP1 Bacillus subtilis procapsid II 600.0 7 VIRION OTHER false false Major capsid protein Bacillus phage SPP1 0.035258425999999995 7 Bacillus subtilis LEVO AYTKISDVIVPELFNPYVINTTTQLSAFFQSGIAATDDELNALAKKAGGGSTLNMPYWNDLDGDSQVLNDTDDLVPQKIN AGQDKAVLILRGNAWSSHDLAATLSGSDPMQAIGSRVAAYWAREMQKIVFAELAGVFSNDDMKDNKLDISGTADGIYSAE TFVDASYKLGDHESLLTAIGMHSATMASAVKQDLIEFVKDSQSGIRFPTYMNKRVIVDDSMPVETLEDGTKVFTSYLFGA GALGYAEGQPEVPTETARNALGSQDILINRKHFVLHPRGVKFTENAMAGTTPTDEELANGANWQRVYDPKKIRIVQFKHR LQA Q38582 singleParticle particle 7.0 Quantifoil R2/2 COPPER 400 GLOW DISCHARGE AIR ETHANE 100 293 FEI VITROBOT MARK II FEI POLARA 300 OTHER BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.3 0.9 0.9 3.0 3.0 39000.0 39000.0 GATAN 910 MULTI-SPECIMEN SINGLE TILT CRYO TRANSFER HOLDER NITROGEN 70.0 85.0 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 3840 3712 4-21 1 7000 5.0 1.1 1 11745 I EXACT BACK PROJECTION 4.2 FSC 0.5 CUT-OFF IMAGIC 5 8808 ANGULAR RECONSTITUTION IMAGIC 5 ANGULAR RECONSTITUTION IMAGIC 5 1.0 IMAGIC 5 emd_10002.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 600 600 600 -300 -300 -300 600 600 600 785.99994 785.99994 785.99994 90.0 90.0 90.0 X Y Z 0.0 15.11228 0.16541384 0.8142602 1.31 1.31 1.31 4.5 AUTHOR Intemediate state of the SPP1 procapsid, prior DNA packaging FLEXIBLE FIT REAL