2020-12-09 REL PDBj PDBj PDBj 2020-02-03 2020-02-19 2020-02-19 2020-12-09 National Health and Medical Research Council (NHMRC, Australia) 1120919 Australia National Health and Medical Research Council (NHMRC, Australia) 1150083 Australia National Health and Medical Research Council (NHMRC, Australia) 1159006 Australia Japan Science and Technology 18069571 Japan Japan Society for the Promotion of Science (JSPS) 18H06043 Japan Cryo-EM map of PAC1 receptor bound to PACAP38 and Gs protein Liang YL Belousoff MJ Zhao P Danev R Sexton PM Wootten D Liang YL Belousoff MJ Zhao P Koole C Fletcher MM Truong TT Julita V Christopoulos G Xu HE Zhang Y Khoshouei M Christopoulos A Danev R Sexton PM Wootten D Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation. Mol.Cell US 77 656 668.e5 2020 32004469 doi:10.1016/j.molcel.2020.01.012 1097-2765 2168 MOCEFL EMD-20278 other EM volume
Earlier map
 EMD-0993 associated EM volume PAC1 receptor bound to PACAP38 and Gs protein PAC1 receptor bound to PACAP38 and Gs protein 0 Homo sapiens Trichoplusia ni 150 singleParticle particle 4.35 7.4 Quantifoil R1.2/1.3 COPPER/RHODIUM 200 GLOW DISCHARGE AIR ETHANE 100 277 FEI VITROBOT MARK IV
blot time 10 s.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.4 0.4 1.6 1.6 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE GIF Quantum LS 25 GATAN K3 BIOQUANTUM (6k x 4k) 1 7650 3.715 64.0
Volta phase plate images: 4032; Conventional defocus images: 3618
 1 5500000 Gctf
20A low-pass filtered GPCR map from previous experiment.
 1 C1 2.65 FSC 0.143 CUT-OFF RELION 3 390000 MAXIMUM LIKELIHOOD RELION 3 MAXIMUM LIKELIHOOD RELION 3 3 RELION 3 emd_0993.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 176 176 176 0 0 0 176 176 176 212.432 212.432 212.432 90.0 90.0 90.0 X Y Z -0.20266752 0.37457576 0.00000797852 0.011056929 1.207 1.207 1.207 0.06 AUTHOR Sharpened map of PAC1 receptor bound to PACAP38 and Gs protein emd_0993_msk_1.map emd_0993_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 176 176 176 0 0 0 176 176 176 212.432 212.432 212.432 90.0 90.0 90.0 X Y Z -0.05813296 0.13243423 0.00000417609 0.004591972 1.207 1.207 1.207 AUTHOR Unprocessed map of PAC1 receptor bound to PACAP38 and Gs protein emd_0993_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 176 176 176 0 0 0 176 176 176 212.432 212.432 212.432 90.0 90.0 90.0 X Y Z -0.05651434 0.13174637 0.0000087717 0.005051473 1.207 1.207 1.207 AUTHOR Unprocessed half-map of PAC1 receptor bound to PACAP38 and Gs protein emd_0993_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 176 176 176 0 0 0 176 176 176 212.432 212.432 212.432 90.0 90.0 90.0 X Y Z -0.06502448 0.14218988 0.00000718532 0.005059672 1.207 1.207 1.207 AUTHOR Unprocessed half-map of PAC1 receptor bound to PACAP38 and Gs protein