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    <admin>
        <current_status>
            <date>2024-10-30</date>
            <code>REL</code>
            <processing_site>PDBj</processing_site>
        </current_status>
        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBj</last_processing>
        </sites>
        <key_dates>
            <deposition>2020-01-16</deposition>
            <header_release>2020-05-13</header_release>
            <map_release>2020-05-13</map_release>
            <update>2024-10-30</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Chinese Academy of Sciences</funding_body>
                <code>XDA24020302</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Ministry of Science and Technology (MoST, China)</funding_body>
                <code>2018YFA0903100</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Ministry of Science and Technology (MoST, China)</funding_body>
                <code>2016YFA0400900</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>31630001</code>
                <country>China</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>31621002</code>
                <country>China</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-EM structure of RuBisCO-Raf1 from Anabaena sp. PCC 7120</title>
        <authors_list>
            <author>Xia LY</author>
            <author>Jiang YL</author>
        </authors_list>
        <keywords>RuBisCO, Chaperone, Raf1, CHAPERONE-LYASE complex</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Xia LY</author>
                    <author order="2">Jiang YL</author>
                    <author order="3">Kong WW</author>
                    <author order="4">Sun H</author>
                    <author order="5">Li WF</author>
                    <author order="6">Chen Y</author>
                    <author order="7">Zhou CZ</author>
                    <title>Molecular basis for the assembly of RuBisCO assisted by the chaperone Raf1.</title>
                    <journal_abbreviation>Nat.Plants</journal_abbreviation>
                    <country>UK</country>
                    <volume>6</volume>
                    <first_page>708</first_page>
                    <last_page>717</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">32451445</external_references>
                    <external_references type="DOI">doi:10.1038/s41477-020-0665-8</external_references>
                    <external_references type="ISSN">2055-0278</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6lrr</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Ternary complex of RuBisCO with the chaperone Raf1</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Ternary complex of RuBisCO with the chaperone Raf1</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="103690">Nostoc sp. PCC 7120</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.9</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>All5250 protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="103690">Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)</organism>
                    <strain>PCC 7120 / SAG 25.82 / UTEX 2576</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.018391881</theoretical>
                </molecular_weight>
                <number_of_copies>8</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="866768">Escherichia coli 'BL21-Gold(DE3)pLysS AG'</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>QRPAPIPPFFRFDTEDELPRIVPVVGQLPLKAEELKAVPLVEEIEPFRLVKFSGEQAWVALPGWQVLLAAEDPVTILATS
DRFPKQNQTEPGPVLVVVDRSQREWNDFSYFVVDHDGELDFQWFETKPEFPILGKVIILVRPRRILDENVTKDSWQIDE</string>
                    <external_references type="UNIPROTKB">Q8YLP6</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Ribulose bisphosphate carboxylase small chain</name>
                <natural_source database="NCBI">
                    <organism ncbi="103690">Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)</organism>
                    <strain>PCC 7120 / SAG 25.82 / UTEX 2576</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.012840724999999999</theoretical>
                </molecular_weight>
                <number_of_copies>8</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="866768">Escherichia coli 'BL21-Gold(DE3)pLysS AG'</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MQTLPKERRYETLSYLPPLTDVQIEKQVQYILSQGYIPAVEFNEVSEPTELYWTLWKLPLFGAKTSREVLAEVQSCRSQY
PGHYIRVVGFDNIKQCQILSFIVHKPSRY</string>
                    <external_references type="UNIPROTKB">P06514</external_references>
                </sequence>
                <ec_number>4.1.1.39</ec_number>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Ribulose bisphosphate carboxylase large chain</name>
                <natural_source database="NCBI">
                    <organism ncbi="103690">Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)</organism>
                    <strain>PCC 7120 / SAG 25.82 / UTEX 2576</strain>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.053112124999999996</theoretical>
                </molecular_weight>
                <number_of_copies>8</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="866768">Escherichia coli 'BL21-Gold(DE3)pLysS AG'</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSYAQTKTQTKSGYKAGVQDYRLTYYTPDYTPKDTDILAAFRVTPQPGVPFEEAAAAVAAESSTGTWTTVWTDLLTDLDR
YKGRCYDIEPVPGEDNQFIAYIAYPLDLFEEGSITNVLTSIVGNVFGFKALRALRLEDIRFPVAYIKTFQGPPHGIQVER
DKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSAPFQRWRDRFLFVADAITKAQAETGEIKGHY
LNVTAPTCEEMLKRAEYAKELKQPIIMHDYLTAGFTANTTLARWCRDNGVLLHIHRAMHAVIDRQKNHGIHFRVLAKALR
LSGGDHIHTGTVVGKLEGERGITMGFVDLLRENYVEQDKSRGIYFTQDWASLPGVMAVASGGIHVWHMPALVEIFGDDSV
LQFGGGTLGHPWGNAPGATANRVALEACVQARNEGRNLAREGNDVIREAAKWSPELAVACELWKEIKFEFEAMDTV</string>
                    <external_references type="UNIPROTKB">P00879</external_references>
                </sequence>
                <ec_number>4.1.1.39</ec_number>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>SPOT SCAN</illumination_mode>
                    <imaging_mode>DIFFRACTION</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>SUPER-RESOLUTION</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">50.0</average_electron_dose_per_image>
                        </image_recording>
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                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="PDB ENTRY">
                    <pdb_model>
                        <pdb_id>6KKN</pdb_id>
                    </pdb_model>
                </startup_model>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C4</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.37</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                        </software>
                    </software_list>
                    <number_images_used>149382</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>ANGULAR RECONSTITUTION</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_0959.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>240</col>
            <row>240</row>
            <sec>240</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>240</x>
            <y>240</y>
            <z>240</z>
        </spacing>
        <cell>
            <a units="Å">249.59999</a>
            <b units="Å">249.59999</b>
            <c units="Å">249.59999</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-0.11982656</minimum>
            <maximum>0.20713094</maximum>
            <average>0.00030735866</average>
            <std>0.0089101745</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.04</x>
            <y units="Å">1.04</y>
            <z units="Å">1.04</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.0209</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0959::::</label>
    </map>
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            <modelling>
                <initial_model>
                    <access_code>6KKM</access_code>
                    <chain>
                        <source_name>PDB</source_name>
                        <initial_model_type>experimental model</initial_model_type>
                    </chain>
                </initial_model>
                <refinement_protocol>OTHER</refinement_protocol>
            </modelling>
        </modelling_list>
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