2024-03-27 REL PDBj PDBj PDBj 2020-01-02 2020-02-26 2020-02-26 2024-03-27 Ministry of Education (MoE, Singapore) MOE2012-T3-1-008 Singapore National Research Foundation (NRF, Singapore) NRF-NRFI2016-01 Singapore National Research Foundation (NRF, Singapore) NRF2015NRF-CRP001-063 Singapore Cryo-EM structure of immature Zika virus Tan TY Fibriansah G immature Zika virus, virus, flavivirus Tan TY Fibriansah G Kostyuchenko VA Ng TS Lim XX Zhang S Lim XN Wang J Shi J Morais MC Corti D Lok SM Capsid protein structure in Zika virus reveals the flavivirus assembly process. Nat Commun UK 11 895 895 2020 32060358 doi:10.1038/s41467-020-14647-9 2041-1723 6lnu FULLOVERLAP Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013 Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013 0 1 2
The virus was isolated from Zika patient. The immature Zika virus was grown in Aedes Albopictus clone C6/36 cell.
Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013 H/PF/2013 VIRION STRAIN true false Genome polyprotein Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013) isolate ZIKV/Human/French Polynesia/10087PF/2013 0.054444051 3 LEVO IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEA YLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKE ALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAE TLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHW HRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTK NGSISLMCLALGGVLIFLSTAVSA A0A024B7W1 3.4.21.91 Genome polyprotein Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013) isolate ZIKV/Human/French Polynesia/10087PF/2013 0.018561266 3 LEVO RGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQIMDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKK GEARRSRRAVTLPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKVIYLVMILLIAP AYS A0A024B7W1 3.4.21.91 singleParticle particle 8.0
10 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA, pH 8
 ETHANE
the virus sample was incubated at 37deg C for 30 min prior to sample blotting onto the grid.
 FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 3.5 59000.0 FEI FALCON II (4k x 4k) 18.0 1 EMD-8508 I 9.0 FSC 0.143 CUT-OFF RELION 7922 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 emd_0933.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 560 560 560 -280 -280 -280 560 560 560 750.4 750.4 750.4 90.0 90.0 90.0 X Y Z -5.1514034 9.527009 -0.008810329 0.9886185 1.34 1.34 1.34 1.85 AUTHOR 4B03 PDB experimental model FLEXIBLE FIT