2024-11-13 REL PDBj PDBj PDBj 2019-12-10 2020-02-26 2020-02-26 2024-11-13 cryo-EM structure of GPR52-miniGs-NB35 Li M Wang N G-protein coupled receptor, orphan GPCR, self-activation, cryo-EM, MEMBRANE PROTEIN Lin X Li M Wang N Wu Y Luo Z Guo S Han GW Li S Yue Y Wei X Xie X Chen Y Zhao S Wu J Lei M Xu F Structural basis of ligand recognition and self-activation of orphan GPR52. Nature UK 579 152 157 2020 32076264 doi:10.1038/s41586-020-2019-0 1476-4687 0006 NATUAS 6li3 FULLOVERLAP GPR52-miniGs-NB35 GPR52-miniGs-NB35 0 1 2 3 4 5 GPR52-GsBeta-GsGamma 1 1 3 4 Homo sapiens GsAlpha-NB35 1 2 5 Homo sapiens G-protein coupled receptor 52 Homo sapiens 0.039128777000000003 1 Spodoptera frugiperda LEVO MNESRWTEWRILNMSSGIVNVSERHSCPLGFGHYSVVDVCIFETVVIVLLTFLIIAGNLTVIFVFHCAPLLHHYTTSYFI QTMAYADLFVGVSCLVPTLSLLHYSTGVHESLTCQVFGYIISVLKSVSMWCLACISVDRYLAITKPLSYNQLVTPCRLRI CIILIWIYSCLIFLPSFFGWGKPGYHGDIFEWCATSWLTSAYFTGFIVCLLYAPAAFVVCFTYFHIFKICRQHTKEINDR RARFPSHEVDSSRETGHSPDRRYAMVLFRITSVFYMLWLPYIIYFLLESSRVLDNPTLSFLTTWLAISNSFCNPVIYSLS NSVFRLGLRRLSETMCTSCM Q9Y2T5 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Homo sapiens 0.028907684 1 Escherichia coli BL21(DE3) LEVO NSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVDK VNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKS KIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRDIIQRM HLRQYELL P63092 P63092 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Homo sapiens 0.03741693 1 Spodoptera frugiperda LEVO MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLI IWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTS SGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGV TDDGMAVATGSWDSFLKIWN P62873 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Homo sapiens 0.007845078 1 Spodoptera frugiperda LEVO MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFSAIL P59768 nanobody Nb35 Lama glama 0.016054231999999998 1 Escherichia coli BL21(DE3) LEVO MKYLLPTAAAGLLLLAAQPAMAMQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGAS ISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTV singleParticle particle 7.5 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI FALCON III (4k x 4k) 40.0 1 3.32 FSC 0.143 CUT-OFF 651465 COMMON LINE MAXIMUM LIKELIHOOD emd_0902.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 279.04 279.04 279.04 90.0 90.0 90.0 X Y Z -0.2152461 0.35082987 0.000006067563 0.0069224434 1.09 1.09 1.09 0.036 AUTHOR None