Cryo-EM structure of a class A GPCR monomer

2024-10-16 REL PDBj PDBj PDBj 2019-12-03 2020-09-02 2020-09-02 2024-10-16 Cryo-EM structure of a class A GPCR monomer Liu ZJ Hua T G protein coupled-receptor, MEMBRANE PROTEIN Liu K Wu L Yuan S Wu M Xu Y Sun Q Li S Zhao S Hua T Liu ZJ Structural basis of CXC chemokine receptor 2 activation and signalling. Nature UK 585 135 140 2020 32610344 doi:10.1038/s41586-020-2492-5 1476-4687 0006 NATUAS 6lfo FULLOVERLAP Ternary complex of chemokine-monomer, GPCR and G protein Ternary complex of chemokine-monomer, GPCR and G protein 0 1 2 3 4 5 6 Homo sapiens 0.15 Guanine nucleotide-binding protein G(i) subunit alpha-1 Homo sapiens 0.040283836 1 Spodoptera frugiperda LEVO GCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSI IAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDR IAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMN RMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYT HFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF P63096 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Homo sapiens 0.038045629 1 Spodoptera frugiperda LEVO IGRARGFSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSAS QDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDD NQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICF FPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNR VSCLGVTDDGMAVATGSWDSFLKIWN P62873 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Homo sapiens 0.007861143 1 Spodoptera frugiperda LEVO MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL P59768 Interleukin-8 Homo sapiens 0.007600903 1 Spodoptera frugiperda LEVO SAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKF P10145 C-X-C chemokine receptor type 2 Homo sapiens 0.040798121 1 Spodoptera frugiperda LEVO MEDFNMESDSFEDFWKGEDLSNYSYSSTLPPFLLDAAPCEPESLEINKYFVVIIYALVFLLSLLGNSLVMLVILYSRVGR SVTDVYLLNLALADLLFALTLPIWAASKVNGWIFGTFLCKVVSLLKEVNFYSGILLLACISVDRYLAIVHATRTLTQKRY LVKFICLSIWGLSLLLALPVLLFRRTVYSSNVSPACYEDMGNNTANWRMLLRILPQSFGFIVPLLIMLFCYGFTLRTLFK AHMGQKHRAMRVIFAVVLIFLLCWLPYNLVLLADTLMRTQVIQETCERRNHIDRALDATEILGILHSCLNPLIYAFIGQK FRHGLLKILAIHGLISKDSLPKDSRPSFVGSSSGHTSTTL P25025 scFv16 Homo sapiens 0.027784896 1 Spodoptera frugiperda LEVO DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLF LQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCR SSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG AGTKLELKAAAHHHHHHHH CHOLESTEROL 0.000386654 1 CLR singleParticle particle 1 7.5 Homemade GOLD 300 CARBON HOLEY GLOW DISCHARGE 40 ETHANE 100 277 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.8 0.8 2.0 2.0 130000.0 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 20 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 7420 7676 5 9004 0.8 8.0 1 7903150 6N4B C1 3.4 FSC 0.143 CUT-OFF cryoSPARC 2.11 276958 MAXIMUM LIKELIHOOD cryoSPARC 2.11 MAXIMUM LIKELIHOOD cryoSPARC 2.11 4 150000.0 cryoSPARC 2.11 emd_0879.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 266.24 266.24 266.24 90.0 90.0 90.0 X Y Z -0.6928249 1.1548566 0.00009181072 0.032701112 1.3866667 1.3866667 1.3866667 0.1708 AUTHOR ::::EMDATABANK.org::::EMD-0879:::: 6N4B PDB experimental model AB INITIO MODEL Correlation coefficient REAL 93.700000000000003