2020-12-09 REL PDBj PDBj PDBj 2019-11-24 2019-12-18 2019-12-18 2020-12-09 Ministry of Education (Singapore) R-154-000-B58-112 Singapore Ministry of Education (Singapore) R-154-000-B42-114 Singapore Volta electron cryotomograms of cryosections of fission yeast Schizosaccharomyces pombe cells, in G0 quiescence and arrested in G1 phase. Cai S Tan Z Nie X Shi J Gan L Cai S Tan Z Nie X Shi J Gan L Structural and biochemical analysis of G0 S. pombe nuclei To Be Published 0353 EMD-0875 associated EM volume S. pombe cell in G0 quiescence S. pombe cell in G0 quiescence 0 Schizosaccharomyces pombe MBY99 (972 h-) tomography cell 7
EMM-N (EMM without nitrogen)
 COPPER 200 CARBON CONTINUOUS ETHANE FEI VITROBOT MARK IV
The Vitrobot's cup was used to condense the ethane cryogen. Crimped copper tubes containing cell paste were held ~ 3 cm above the surface of the liquid ethane, then dropped..
BBI 10 OTHER
The cells were self-pressurized frozen in crimped copper tubes.. The value given for _emd_high_pressure_freezing.instrument is Vitrobot cup. This is not in a list of allowed values set(['LEICA EM PACT2', 'LEICA EM PACT', 'EMS-002 RAPID IMMERSION FREEZER', 'OTHER', 'LEICA EM HPM100', 'BAL-TEC HPM 010']) so OTHER is written into the XML file.
 30% dextran Leica UC7/FC7 123 70 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 18000.0 30400.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE FEI FALCON II (4k x 4k) INTEGRATING 4096 4096 14.0 1 61 1.6 1 BACK PROJECTION IMOD 4.9 61 emd_0875.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 4096 4096 146 0 0 -58 4096 4096 146 18841.6 18841.6 671.6 90.0 90.0 90.0 X Y Z -3723 2829 168.68185 266.0802 4.6 4.6 4.6 AUTHOR Volta electron cryotomogram of a cryosection of a G0 Schizosaccharomyces pombe cell emd_0875_additional_1.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 4096 4096 210 0 0 -110 4096 4096 210 18841.6 18841.6 966.0 90.0 90.0 90.0 X Y Z -4820 3541 169.11185 250.9971 4.6 4.6 4.6 AUTHOR Volta electron cryotomogram of a cryosection of a G1-arrested Schizosaccharomyces pombe cell