2024-11-13 REL PDBj PDBj PDBj 2019-11-19 2020-04-29 2020-04-29 2024-11-13 National Natural Science Foundation of China 31870835 China Cryo-EM structure of Dnf1 from Chaetomium thermophilum in the E1-ATP state He Y Xu J P-type ATPase, lipid flippase, LIPID TRANSPORT He Y Xu J Wu X Li L Structures of a P4-ATPase lipid flippase in lipid bilayers. Protein Cell CN 11 458 463 2020 32303992 doi:10.1007/s13238-020-00712-y 1674-8018 6lcr FULLOVERLAP Heterodimer of Dnf1-Cdc50 Heterodimer of Dnf1-Cdc50 0 1 2 Chaetomium thermophilum var. thermophilum DSM 1495 Phospholipid-transporting ATPase Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) DSM 1495 / CBS 144.50 / IMI 039719 0.17537953099999998 1 Saccharomyces cerevisiae LEVO MAPPQEEGGGNGTELSMQRSRWATRRLTVKSGARKRLSLMTRAQAKNSATEKRQSGVTDDGSPAADGDQKEGSISSSNNG GSAPRKLYFNLPLPPELKDEEGHPIQQFPRNKIRTAKYTPLSFIPKNLWFQFHNIANIFFLFLVILVIFPIFGGVNPGLN SVPLIVIITVTAIKDAIEDYRRTILDIELNNAPVHRLQGWENVNVEKDNVSLWRRFKKANSRFFGSIWHLIERLWKEDAQ SMRQRFASADPRMSIETRTAPWDPSHRRSVASHTEEIQMTPVPSPVPHDPDVPTVSSAIENEATLLQNLKGDLINHEIPV SGKARFHKDAWKNLVVGDFVRIYNDDELPADIIILATSDPDGACYVETKNLDGETNLKVRQALRCGRTLKHARDCERAQF VIESEPPQPNLYKYNGAIRWKQRVPWDPHGEPREMSEPIGIDNLLLRGCHLRNTEWALGVVVFTGHDTKIMMNAGITPSK RARIARELNFNVICNFGILLIMCLIAAIANGIAWGKTDASLAWFEYGSIGGTPALTGFITFWAAVIVFQNLVPISLYISL EIVRTLQAFFIYSDVGMYYEKIDQPCIPKSWNISDDVGQIEYIFSDKTGTLTQNVMEFKKATINGQPYGEAYTEAQAGMD RRRGINVEEEAKVIREEIAAAKVRAIRGLRELHDNPYLHDEDMTFIAPDFVEDLAGKNGPEQQQATEHFMLALALCHTVV AEKQPGDPPKMIFKAQSPDEAALVATARDMGFTVLGMSDGGINVNVMGKDMHFPVLSIIEFNSSRKRMSTIVRMPDGRIL LFCKGADSVIYSRLKKGEQADMRRETAQHLEMFAVEGLRTLCIAERELSEEEYREWRREHDLAATALENREEKLEEVADK IERDLTLLGGTAIEDRLQDGVPDTIALLADAGIKLWVLTGDKVETAINIGFSCNLLNNDMDLLRLQVNESDASTEDDYLQ LAEEQLKTNLERFNMTGDDEELKRARKDHNAPSPTYALVIDGFTLRWVLSDSLKQKFLLLCKQCKSVLCCRVSPAQKAAV VSMVKNGLDVMTLSIGDGANDVAMIQEADVGVGIAGEEGRQAVMSSDFAIGQFRFLQRLVLVHGRWSYRRLAETISNFFY KNMIWTWSIFWYQCYCNFDIAYIFEYTYILMFNLFFTSVPVILMGVLDQDVSDTVSLAVPQLYRRGIERKEWTQTKFWLY MIDGVYQSVMSFFIPFIFVVLTPTAAGNGLDVSERTRLGAYIAHPAVITINGYILINTYRWDWLMLLSIVLSDVFIFFWT GVYTATTYSAGFYQAAPQVYQELTFWMCLIVTPALCLLPRLVVKCIQKQRFPYDVDIIREQANRGDFAAADAAAVAALGG PERVEGESLGSLSSSGKGSGRSKKSKHQQYASVDEDRRPIYPPSIATHNTRAQNGSDGTTYIMQSRTSTELQQEMPFDRD REEETPAVRPSIERTRPSYDRIRRSIDRVRPSFEASNDFTSAARLSRIESTHSSLGHTYSHQRESYAGESSGAQQGQEPG QRRFNLATVRKRGLSAFSKKSIDTTEGEPPREPPM G0S196 7.6.2.1 Cdc50 Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) DSM 1495 / CBS 144.50 / IMI 039719 0.046452311999999996 1 Saccharomyces cerevisiae LEVO MAPRRRRGAGQDGSDDGRSDSDAPKNRPPNTAFRQQRMRAWQCVLTPKLIVTVFSILAAIYLGFGAWLTYLAHTVRDLKI DYTDCLTSAPKDDFETIPQNHITAHFSAKDSTFDPYKAQWKTTEREVQVANYTDNRQFCIVRFNIPEDLQPTISFFYYLE NFYQNHRRYVNSFNAKQLLGDAVDGKTINDSTCDPITHDPKGTGKIVYPCGLVANSIFNDTFSSPLALAVRNSSDSSRPY NMTTKGIAWPGLKDLYGKTSYSLDQIVPPPNWERRYKYGYQENNPPPDLKTDELFQNWMMLAAAPNFYKLYQKNDTHPML AGQYEIEIESNFDVTVYKGRKAFVITTLSTMGSRNIWPGIIFLIVGGICLVLDIYFILSFFIWRPRKLGDPSYLSWNQPS APGGHSS G0SDN0 MAGNESIUM ION 2.4305e-05 1 MG PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER 0.000505208 1 ACP O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine 0.000792075 1 P5S 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 1 NAG singleParticle particle 1.5 7.5 ETHANE 100 297 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 NITROGEN GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 57.0 1 2397258 2 C1 3.4 FSC 0.143 CUT-OFF RELION 272912 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION 8 RELION emd_0873.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 284 284 284 0 0 0 284 284 284 299.62 299.62 299.62 90.0 90.0 90.0 X Y Z -0.1073686 0.18286814 0.00015012582 0.0030677575 1.055 1.055 1.055 0.02 AUTHOR FLEXIBLE FIT REAL