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                <funding_body>Ministry of Science and Technology (MoST, China)</funding_body>
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        <title>Structure of a membrane protein</title>
        <authors_list>
            <author>Hattori M</author>
            <author>Jin F</author>
        </authors_list>
        <keywords>channel, TRANSPORT PROTEIN, TRANSPORT PROTEIN-IMMUNE SYSTEM complex</keywords>
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    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Jin F</author>
                    <author order="2">Sun M</author>
                    <author ORCID="0000-0002-0997-3365" order="3">Fujii T</author>
                    <author ORCID="0000-0002-9550-2171" order="4">Yamada Y</author>
                    <author order="5">Wang J</author>
                    <author order="6">Maturana AD</author>
                    <author ORCID="0000-0001-7814-0204" order="7">Wada M</author>
                    <author order="8">Su S</author>
                    <author order="9">Ma J</author>
                    <author order="10">Takeda H</author>
                    <author order="11">Kusakizako T</author>
                    <author order="12">Tomita A</author>
                    <author order="13">Nakada-Nakura Y</author>
                    <author order="14">Liu K</author>
                    <author order="15">Uemura T</author>
                    <author order="16">Nomura Y</author>
                    <author ORCID="0000-0002-6330-2239" order="17">Nomura N</author>
                    <author ORCID="0000-0002-2042-405X" order="18">Ito K</author>
                    <author order="19">Nureki O</author>
                    <author ORCID="0000-0003-2911-5875" order="20">Namba K</author>
                    <author order="21">Iwata S</author>
                    <author order="22">Yu Y</author>
                    <author ORCID="0000-0002-5327-5337" order="23">Hattori M</author>
                    <title>The structure of MgtE in the absence of magnesium provides new insights into channel gating.</title>
                    <journal_abbreviation>Plos Biol.</journal_abbreviation>
                    <country>US</country>
                    <volume>19</volume>
                    <first_page>e3001231</first_page>
                    <last_page>e3001231</last_page>
                    <year>2021</year>
                    <external_references type="PUBMED">33905418</external_references>
                    <external_references type="DOI">doi:10.1371/journal.pbio.3001231</external_references>
                    <external_references type="ISSN">1545-7885</external_references>
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                <pdb_id>6lbh</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
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    <sample>
        <name>MgtE-Fab complex</name>
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            <complex_supramolecule supramolecule_id="1">
                <name>MgtE-Fab complex</name>
                <parent>0</parent>
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                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="274">Thermus thermophilus</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Magnesium transporter MgtE</name>
                <natural_source database="NCBI">
                    <organism ncbi="300852">Thermus thermophilus HB8</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.019249996</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>LVYSEAGPVALWLARVRWLVILILTGMVTSSILQGFESVLEAVTALAFYVPVLLGTGGNTGNQSATLIIRALATRDLDLR
DWRRVFLKEMGVGLLLGLTLSFLLVGKVYWDGHPLLLPVVGVSLVLIVFFANLVGAMLPFLLRRLGVDPALVSNPLVATL
SDVTGLLIYLSVARLLLE</string>
                    <external_references type="UNIPROTKB">Q5SMG8</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Fab light chain</name>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.023837455</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="10090">Mus musculus</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>DVLMTQTPLSLPVSLGDQASISCRSSQSLVHSDGNTYLHWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKI
SRVEAEDLGVYFCSQSTHVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSER
QNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR</string>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Fab heavy chain</name>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.024092789</theoretical>
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                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="10090">Mus musculus</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
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MQLNSLTSEDSAVYYCARGESFSNYEGYYAMDYWGQGTSVIVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEP
VTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR</string>
                </sequence>
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