2021-10-27 REL PDBj PDBj PDBj 2019-10-12 2020-10-14 2020-10-14 2021-10-27 Cryo-EM map for N terminal domain of human MCM8/9 complex Li J Yu D Liu L Zeng H Hu Z Xu H Liu B Liang H Ouyang Q Liu Y Li J Yu D Liu L Liang H Ouyang Q Liu Y Structural study of the N-terminal domain of human MCM8/9 complex. Structure UK 29 1171 1181.e4 2021 34043945 doi:10.1016/j.str.2021.05.006 0969-2126 2005 STRUE6 EMD-0823 other EM volume EMD-0824 associated EM volume Minichromosome Maintenance (MCM) 8/9 Complex Minichromosome Maintenance (MCM) 8/9 Complex 0 Homo sapiens Trichoplusia ni 0.5 singleParticle particle 0.3 7.8 20.0 HEPES 160.0 KCl C-flat-1.2/1.3 4C GOLD 300 GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.7000000000000001 2.7 105000.0 36496.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 QUANTUM (4k x 4k) SUPER-RESOLUTION 7420 7676 1-40 3711 10.0 50.0 1 Gctf 1.06
The CTF correction was performed in Relion automatically, using the defocus values calculated by Gctf software.
Generated by CryoSPARC software
 C1 BACK PROJECTION 6.6 FSC 0.143 CUT-OFF RELION 2.1
The structure is obtained by focused refinement for N terminal domain of MCM 8/9 full complex
184677 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 RELION 2.1 emd_0824.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 219.2 219.2 219.2 90.0 90.0 90.0 X Y Z -0.023522943 0.062801406 0.000586121 0.0033867266 1.37 1.37 1.37 0.02 AUTHOR