2021-07-21 REL PDBj PDBj PDBj 2019-08-09 2020-08-12 2020-08-12 2021-07-21 National Natural Science Foundation of China 31830020 China Ministry of Science and Technology (China) 2017YFA0504702 China Spinach 80S ribosome Zhang J Zhang D Sun L Ji G Huang X Niu T Sun F Zhang J Zhang D Sun L Ji G Huang X Niu T Xu J Ma C Zhu Y Gao N Xu W Sun F VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography. J.Struct.Biol. US 213 107763 107763 2021 34174447 doi:10.1016/j.jsb.2021.107763 1095-8657 0803 JSBIEM Zhang J Zhang D Sun L Ji G Huang X Niu T Sun F VHUT-cryo-FIB, a method to fabricate frozen-hydrated lamella 1of tissue specimen for in situcryo-electron tomography Biorxiv US 2019 doi:10.1101/727149 EMD-0734 associated EM volume Spinach 80S ribosome Spinach 80S ribosome 0
Ribosomes were found in cryo-lamella of spinach leaf tissue.
Spinacia olerac subtomogramAveraging particle 7.0 NITROGEN
A puncher was used to cut a slice at about 2 mm diameter from the leaf of spinach. The leaf was then put in the recess of the carrier and frozen by HPF. A lamella was milled from the frozen sample through FIB. Tilt series data was collected on the lamella.
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 3838 3710 1-20 1 1.0 3.0 1 C1 FOURIER SPACE 34.0 FSC 0.143 CUT-OFF RELION 1.4 923 3 1341 IMOD 4.9.2 RELION 1.4
Particles were manually picked from reconstructed tomogram.
 CTFFIND 4 MAXIMUM LIKELIHOOD RELION 1.4 emd_0734.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 84 84 84 0 0 0 84 84 84 445.2 445.2 445.2 90.0 90.0 90.0 X Y Z -0.2550366 0.97346705 0.0414296 0.15774563 5.3 5.3 5.3 0.52 AUTHOR 6EK0 RIGID BODY FIT REAL