2024-03-27 REL PDBj PDBj PDBj 2019-08-03 2020-01-15 2020-01-15 2024-03-27 Structure of human cardiac thin filament in the calcium free state Fujii T Yamada Y Troponin, Tropomyosin, Actin, Thin filement, Muscle, CONTRACTILE PROTEIN-ACTIN BINDING PROTEIN complex Yamada Y Namba K Fujii T Cardiac muscle thin filament structures reveal calcium regulatory mechanism. Nat Commun UK 11 153 153 2020 31919429 doi:10.1038/s41467-019-14008-1 2041-1723 6kn7 FULLOVERLAP Cardiac muscle thin filament in the calcium free state Cardiac muscle thin filament in the calcium free state 0 1 2 3 4 5 6 Oryctolagus cuniculus Actin, alpha skeletal muscle 1 1 Tropomyosin, Troponin 1 2 3 4 5 6 Actin, alpha skeletal muscle Oryctolagus cuniculus 0.04186261299999999 15 LEVO DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWD DMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVT HNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSY ELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITAL APSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF P68135 Tropomyosin alpha-1 chain Homo sapiens 0.031555053 4 Escherichia coli LEVO LKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNR RIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLV IIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLE KSIDDLEDELYAQKLKYKAISEELDHALNDMTSI P09493 Tropomyosin alpha-1 chain Homo sapiens 0.003528104 4 Escherichia coli LEVO ASMDAIKKKMQMLKLDKENALDRAEQAEADK P09493 Troponin T, cardiac muscle Homo sapiens 0.023023039 2 Escherichia coli LEVO FDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRR KAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAE KFDLQEKFKQQKYEINVLRNRINDNQ P45379 Troponin I, cardiac muscle Homo sapiens 0.019639690999999997 2 Escherichia coli LEVO ISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTK NITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSG MEGRKKKFES P19429 Troponin C, slow skeletal and cardiac muscles Homo sapiens 0.018288287 2 Escherichia coli LEVO DDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMM VRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE P63316 ADENOSINE-5'-DIPHOSPHATE 0.000427201 15 ADP singleParticle filament 0.05 7.5 ETHANE JEOL CRYO ARM 200 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 GATAN K2 SUMMIT (4k x 4k) 65.0 1
EM map of actin filament
 6.6 FSC 0.143 CUT-OFF 21588 PROJECTION MATCHING PROJECTION MATCHING emd_0728.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 444.0 444.0 444.0 90.0 90.0 90.0 X Y Z -0.071018465 0.22550805 -0.000039149214 0.011095973 2.22 2.22 2.22 0.0376 AUTHOR