2020-04-15 REL PDBj PDBj PDBj 2019-07-30 2020-04-15 2020-04-15 2020-04-15 National Natural Science Foundation of China 31770794 China Ministry of Science and Technology (China) China Chinese Academy of Sciences XDB08030202 China Full length S-OPA1 coated liposome tube at nucleotide-free state Zhang D Niu T Zhang Y Sun F Zhang D Zhang Y Ma J Zhu C Niu T Chen W Pang X Zhai Y Sun F Cryo-EM structures of S-OPA1 reveal its interactions with membrane and changes upon nucleotide binding. Elife US 9 2020 32228866 doi:10.7554/eLife.50294 2050-084X EMD-0722 associated EM volume full length S-OPA1 coated liposome tube full length S-OPA1 coated liposome tube 0 1
1mg/ml full length S-OPA1 was incubated with 1mg/ml liposome at room temperature for 30 min.
Homo sapiens Escherichia coli Dynamin-like 120 kDa protein, mitochondrial, short form for isoform 1, Optic atrophy protein 1 (OPA1), short form for isoform 1 Homo sapiens Escherichia coli LEVO GPGSTDRGSESDKHFRKVSDKEKIDQLQEELLHTQLKYQRILERLEKEN KELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAG KTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALR HEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFS ISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPS RIQQIIEGKLFPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVT TRNLSLAVSDCFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNE ILDEVISLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQW TDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAED SLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKN RTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQV YRRHFLKTALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTN TEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK subtomogramAveraging helicalArray 1 8 20.0 Tris 1.0 MgCl2 1.0 EGTA Quantifoil R2/1 COPPER 300 CARBON GLOW DISCHARGE OTHER ETHANE 100 289 FEI VITROBOT MARK IV
The grid was blotted for 3.5s with force 1 before plunging..
1mg/ml full length S-OPA1 was incubated with 1mg/ml liposome at room temperature for 30 min.
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 4.0 5.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 3838 3710 1-12 1 1.0 3.0 1 C1 FOURIER SPACE 31.7 FSC 0.143 CUT-OFF RELION 1.4 436 9 773 IMOD 4.2.9 RELION 1.4 CTFFIND 4 MAXIMUM LIKELIHOOD RELION 1.4 emd_0722.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 -64 -64 -64 128 128 128 348.16 348.16 348.16 90.0 90.0 90.0 X Y Z -0.23188168 0.29639256 0.008297293 0.05131582 2.72 2.72 2.72 0.07 AUTHOR 3SNH A RIGID BODY FIT REAL