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        <key_dates>
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            <header_release>2020-01-15</header_release>
            <map_release>2020-01-15</map_release>
            <update>2025-04-09</update>
        </key_dates>
        <title>F-actin of cardiac thin filament in high-calcium state</title>
        <authors_list>
            <author>Oda T</author>
            <author>Yanagisawa H</author>
        </authors_list>
        <keywords>Cardiac thin filament, CONTRACTILE PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Oda T</author>
                    <author order="2">Yanagisawa H</author>
                    <author order="3">Wakabayashi T</author>
                    <title>Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.</title>
                    <journal_abbreviation>J.Struct.Biol.</journal_abbreviation>
                    <country>US</country>
                    <volume>209</volume>
                    <first_page>107450</first_page>
                    <last_page>107450</last_page>
                    <year>2020</year>
                    <external_references type="PUBMED">31954841</external_references>
                    <external_references type="DOI">doi:10.1016/j.jsb.2020.107450</external_references>
                    <external_references type="ISSN">1095-8657</external_references>
                    <external_references type="CSD">0803</external_references>
                    <external_references type="ASTM">JSBIEM</external_references>
                </journal_citation>
            </primary_citation>
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                    <other>other EM volume</other>
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        </emdb_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6kln</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
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        </pdb_list>
    </crossreferences>
    <sample>
        <name>Cardiac thin filament</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Cardiac thin filament</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                    <strain>ICR</strain>
                    <organ>Heart</organ>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Actin, alpha skeletal muscle</name>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                    <organ>Heart</organ>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.041875633</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIE(HIC)GII
TNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSG
DGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSL
EKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKE
ITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF</string>
                    <external_references type="UNIPROTKB">P68134</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="2">
                <name>MAGNESIUM ION</name>
                <molecular_weight>
                    <theoretical units="MDa">2.4305e-05</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <formula>MG</formula>
            </ligand>
            <ligand macromolecule_id="3">
                <name>ADENOSINE-5'-DIPHOSPHATE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000427201</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <formula>ADP</formula>
            </ligand>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>filament</aggregation_state>
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                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.1</concentration>
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                        <ph>7.2</ph>
                    </buffer>
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                        <model>Quantifoil R1.2/1.3</model>
                        <material>COPPER</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">60</time>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <instrument>FEI VITROBOT MARK IV</instrument>
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                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
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                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.1</nominal_defocus_min>
                    <nominal_defocus_max units="µm">1.1</nominal_defocus_max>
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                        <phase_plate>VOLTA PHASE PLATE</phase_plate>
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                            <name>GIF Quantum LS</name>
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                            <average_exposure_time units="s">5.6</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">60.0</average_electron_dose_per_image>
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                </single_particle_microscopy>
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                    <emdb_id>EMD-7938</emdb_id>
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                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
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        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>264</col>
            <row>264</row>
            <sec>264</sec>
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        <spacing>
            <x>264</x>
            <y>264</y>
            <z>264</z>
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        <cell>
            <a units="Å">282.48</a>
            <b units="Å">282.48</b>
            <c units="Å">282.48</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
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            <minimum>-0.007977668</minimum>
            <maximum>0.04777901</maximum>
            <average>-0.00023301365</average>
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        <pixel_spacing>
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            <y units="Å">1.07</y>
            <z units="Å">1.07</z>
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            <contour primary="true">
                <level>0.017</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0712::::</label>
        <annotation_details>F-actin of cardiac thin filament, high calcium state</annotation_details>
    </map>
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