2024-03-27 REL PDBj PDBj PDBj 2019-01-10 2019-02-20 2019-02-20 2024-03-27 Japan Society for the Promotion of Science JP25116002 Japan Japan Society for the Promotion of Science JP15H04344 Japan Japan Agency for Medical Research and Development (AMED) JP18am0101076 Japan Japan Agency for Medical Research and Development (AMED) JP18am0101082 Japan Japan Science and Technology JPMJCR16G1 Japan Ehara H Kujirai T nucleosome, chromatin, RNA polymerase, TRANSCRIPTION, TRANSCRIPTION-RNA-DNA complex Ehara H Kujirai T Fujino Y Shirouzu M Kurumizaka H Sekine SI Science US 363 744 747 2019 30733384 doi:10.1126/science.aav8912 1095-9203 0038 SCIEAS 6j51 FULLOVERLAP RNA polymerase II elongation complex bound with Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome, weak Elf1 (+1 position) RNA polymerase II elongation complex bound with Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome, weak Elf1 (+1 position) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Komagataella phaffii strain GS115 / ATCC 20864 DNA-directed RNA polymerase subunit Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.19410742199999997 1 LEVO MSQFPYSSAPLRSVKEVQFGLLSPEEIRAISVVKIEYPEIMDESRQRPREGGLNDPKLGSIDRNFKCQTCGEGMAECPGH FGHMELAKPVFHIGFIPKIKKVCECICMNCGKLLLDETNPTMAQAIRIRDPKKRFNAVWQLCKTKMVCEADAPVDEYSEQ KVVSRGGCGNTQPVVRKDGMKLWGTWKKSGFSDRDAQPERKLLTPGEILNVFKHISPEDCFRLGFNEDYARPEWMIITVL PVPPPQVRPSIAMDETTQGQDDLTHKLSDILKANINVQKLEMDGSPQHIINEVEQLLQFHVATYMDNDIAGQPQALQKSG RPVKAIRARLKGKEGRLRGNLMGKRVDFSARTVISGDPNLELDQVGVPISIAKTLSYPETVTQYNIHRLTEYVRNGPNEH PGAKYVIRDNGDRIDLRYHKRAGDIVLQYGWKVERHLMDDDPVLFNRQPSLHKMSMMAHRVKVMPYSTFRLNLSVTSPYN ADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPVMGIVQDTLCGVRKMTLRDTFIEYEQVMNMLFWVPSWDGV VPQPAILKPKPLWTGKQLLSIAIPSGIHLQRTDGGNSLLSPKDNGMLIVDGKVMFGVVDKKTVGSGGGGLIHTVMREKGP KICAELFGNIQKVVNYWLLHNGFSIGIGDAIADASTMKEITHAISSAKEQVQEIIYKAQHNELELKPGMTLRESFEGEVS RTLNDARDSAGRSAEMNLKDLNNVKQMVSAGSKGSFINIAQMSACVGQQMVEGKRIAFGFADRSLPHFTKDDFSPESKGF VENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGDIIQFLYGEDGLDGTQVE RQTIDTIPGSDKAFHKRYYVDLMDEKNSIKPDVIEYAADILGDVELQKELNSEYEQLVSDRKFLREIVFVNGDHNWPLPV NLRRIIQNAQQIFHLDRAKASDLTIPEIIHGVRDLCKKLFVLRGENELIKEAQQNATSLFQCLVRARLATRRILEEFRLN RDAFEWVLGTIEAQFQRSLVHPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTV YLDREIALDIEKAKVIQSSIEYTTLKNVTSATEIYYDPDPTSTVIEEDFDTVEAYFSIPDEKVEETIDKQSPWLLRLELD RARMLDKQLTMNQVADKISEVFSDDLFVMWSEDNADKLIIRCRVIRDPKAMDEELEAEEDQMLKRIEAHMLDLIALRGIP GISKVYMVKHKVSVPDESGEYKNEELWALETDGINLAEVMAVPGVDSSRTYSNSFVEILSVLGIEATRSSLYKEILNVIA FDGSYVNYRHMALLVDVMTSRGYLMAITRHGINRADTGALMRCSFEETVEILFEAGAAAELDDCRGVSENVMLGQLAPMG TGAFDVMIDEKLLTSLPADYAPTMPLFKGKATQGSATPYDNNAQYDDEFNHDDVADVMFSPMAETGSGDDRSGGLTEYAG IQSPYQPTSPGLSATSPGFAPTSPGFAPTSPRYSPTSPGYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP SYSPTSPSYSPTSPSYSPTSPSYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYS PTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPASPQYSPSRHSPNGESKEGE C4R4Y0 2.7.7.6 DNA-directed RNA polymerase subunit beta Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.13974609400000002 1 LEVO MSYDPYSIDDTITTEDCWTVISAFFEEKGLVSQQLDSFDEFMETSIQDLVWEEPRLILDQPAQHTNEKDNINKRYEIRFG KIYLSRPTMTEADGTTHAMFPQEARLRNLTYSSPVYLDMEKSMFTSIDDEGNPNATLDWQQVHEPIKDGVEEGNKVHIGK VPIMLRSKFCSLRTLDEVDLYKMKECPYDMGGYFVINGSEKVLIAQERSAANIVQVFKKAAPSPISHVAEIRSALEKGSR LISTMQIKLYGREDKGTGRTIKATLPYVKQDIPIVIVFRALGVVPDGEILQHICYDENDWQMLEMLKPCIEEGFVIQDKE VALDFIGRRGSAALGIRREKRIQYAKDILQKELLPHITQEEGFETRKTFFLGYMVNRLLLCALERKDQDDRDHFGKKRLD LAGPLLANLFRILFRKLTREIYRYMQRCIETDRDFNLNLAVKSTTITSGLKYSLATGNWGEQKKAMSSRAGVSQVLNRYT YSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLLSGISIGSPSEPIINFLEEWGMEPLE DYDPAQHTKSTRIFVNGVWTGIHRDPSMLVSTMRDLRRSGAISPEVSIIRDIREREFKIFTDVGRVYRPLFIVEDDESKD NKGELRITKEHIRKIQQGYDDDAMNDDSEEQEQDVYGWSSLVTSGVIEYVDGEEEETIMIAMTPEDLQTRSLEQKEIDLN DTAKRIKPEMSTSSHHTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQ KPLAKTQAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKRFGISIVEEFEKPTRA TTLRLKHGTYEKLDEDGLIAPGVRVSGDDIIIGKTTPIPPDTEELGQRTKYHTKRDASTPLRSTENGIVDQVLLTTNQEG LKFVKVRMRTTKVPQIGDKFASRHGQKGTIGVTYRHEDMPFSAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVGSIRGY EGDATPFTDLTVDAVSNLLRDNGYQSRGFEVMYNGHTGKKLMAQVFFGPTYYQRLRHMVDDKIHARARGPVQVLTRQPVE GRSRDGGLRFGEMERDCMIAHGAAGFLKERLMEASDAFRVHVCGICGLMSVIANLKKNQFECRSCKNKTNIYQLHIPYAA KLLFQELMAMNIAPRLYTERSGVSMRS C4QZQ7 2.7.7.6 RNA polymerase II third largest subunit B44, part of central core Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.034216292999999995 1 LEVO MSKEPKVNIINAQDDEVELMLSDVNLSLANSLRRTMLAEVPTLAIDLVEIKMNTSVLADEFISHRLGLIPLVSEDVEEMK YSRDCTCEDYCDECSVVLELSARHEGEEGTTDVYSSSLIKVSGPGNLNVGEPVRRDDYDQGILLCKLRNHQELNIRCIAK KGIAKEHAKWSPCSAIAFEYDPHNKLKHTDFWFEVDAKKEWPDSKYATWEEPPKPGEVFDYKAKPNRFYMTVETTGSLKA NQVFSRGIKTLQEKLANVLFELENSRPANTTAYGGATAYGGQTVYGRETSYGGNTNYGDYNAPY C4R7L2 RNA polymerase II subunit B32 Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.02062298 1 LEVO MNVSTSTVGARRRRAKQQVDDEENATLLRLGPEFALKQYDHDGNEHDLIALSLSESRLLIREALKARSRARNGGVDIESS NGEIDDDELAKVTSGAVANGVVKKTLDYLNTFARFKDEETCTAVDQLLHNSSDCSVLHPFEIAQLSSLGCEDVDEAITLI PSLAAKKEVNLQRILDELNRLEDPYK C4R2U9 RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.024962679999999998 1 LEVO MEDNNRIISRLWRSFRTVKEMAADRGYFISQEEMDQSLEEFRSKICDSMGNPQRKLMSFLANPTPEALEKYSDLGTLWVE FCDEPSVGIKTMRNFCLRIQEKNFSTGIFIYQNNITPSANKMIPTVSPAIIETFQESDLVVNITHHELVPKHIRLSDGEK SQLLQRYKLKESQLPRIQREDPVARYLGLKRGQVVKIIRRSETSGRYASYRICL C4R3P8 RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.017803588 1 LEVO MSEDEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKAHRKRSNKELAILKEERT TTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMKELSQRKIPLVIRRYLPDGSYEDWGCDELIVDN C4R1V1 RNA polymerase II subunit Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.018802625 1 LEVO MFFLKDLSLILTLHPSYFGPQMNQYLREKLLTDVEGTCTGQFGYIVTVLDGMNIDVGKGRIIPGSGSAEFEVKYRAVVWK PFKGEVVDAIVSNVSPIGFFADVGPLNVFVSTRLIPDNLVYNPSNSPPAYMSNDELITKGSKVRLKVVGTRTDVNEIYAI GSIKEDFLGAI C4R9A1 DNA-directed RNA polymerases I, II, and III subunit RPABC3 Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.016249219999999998 1 LEVO MSSALFDDIFTVQTVDNGRYNKVSRIIGISTTNSAIKLTLDINNEMFPVSQDDSLTVTLANSLSLDGEDESANFSKSWRP PKPTDKSLADDYDYVMFGTVYKFEEGDEDKIKVYVSFGGLLMCLEGGYKSLASLKQDNLYILIRR C4R273 DNA-directed RNA polymerase subunit Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1 0.013612319999999999 1 LEVO MASFRFCLECNNMLYPKEDKENQRLLYSCRNCDYTELAEDPKVYRHELITNIGETAGIVDDIGQDPTLPRSDKECPECHS RDCVFFQSQQRRKDTNMTLFYVCLNCKKTFRDESE F2QPE6 RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.008554064 1 LEVO MIIPVRCFSCGKVVGDKWDAYLRLLEEGKQEGDALDELKLKRYCCRRMVLTHVDLIEKFLRYNPLEKKDFDS C4R009 RNA polymerase II subunit B12.5 Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.013832896 1 LEVO MNAPDRFELFILPDDVPKLKITPDSRVPNCIIIKFEREDHTLANLLREELALYPDVTFVAYKVEHPLFANFVMRLQTEEG TRPKQALERACASIINKLKTLDHKFNEEWNIKNFSLND C4R3Z5 RNA polymerase subunit ABC10-alpha Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1 0.007862048 1 LEVO MSREGFVAPSGTDLAAAASGVAPNKHYGVKYTCGACAHNFSLNKSDPVRCKECGHRVIYKARTKRMIQFDAR F2QMI1 RNA (5'-R(P*CP*CP*UP*GP*GP*UP*GP*UP*CP*UP*UP*GP*GP*GP*UP*G)-3') synthetic construct 0.005124023 1 CCUGGUGUCUUGGGUG DNA (198-MER) synthetic construct 0.060869977 1 (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA)(DC)(DA)(DC)(DC)(DC)(DA)(DA)(DG)(DA)(DC)(DA)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC)(DG)(DA)(DG)(DA)(DC)(DA)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DA) (DA)(DC)(DG)(DA)(DA)(DA)(DA)(DC)(DG)(DG)(DC)(DC)(DA)(DC)(DC)(DA)(DC)(DC)(DC)(DA) (DA)(DA)(DC)(DA)(DC)(DA)(DC)(DC)(DA)(DA)(DA)(DC)(DA)(DC)(DA)(DA)(DG)(DA)(DG)(DC) (DT)(DA)(DA)(DT)(DT)(DG)(DA)(DC)(DT)(DG)(DA)(DC)(DG)(DT)(DA)(DA)(DG)(DC) DNA DNA (198-MER) synthetic construct 0.061381906 1 (DG)(DC)(DT)(DT)(DA)(DC)(DG)(DT)(DC)(DA)(DG)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DA)(DT) (DC)(DT)(DT)(DT)(DG)(DT)(DG)(DT)(DT)(DT)(DG)(DG)(DT)(DG)(DT)(DG)(DT)(DT)(DT)(DG) (DG)(DG)(DT)(DG)(DG)(DT)(DG)(DG)(DC)(DC)(DG)(DT)(DT)(DT)(DT)(DC)(DG)(DT)(DT)(DG) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DG)(DT)(DC)(DT)(DC)(DG)(DT)(DG)(DC)(DC)(DT) (DG)(DG)(DT)(DG)(DT)(DC)(DT)(DT)(DG)(DG)(DG)(DT)(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC) (DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG) (DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT) (DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC) (DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT) (DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)(DC)(DT)(DG)(DA)(DT) DNA Transcription elongation factor 1 homolog Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.012606896 1 Escherichia coli LEVO GPGMGKRKSSARKPAPKIKQKLETQFTCLFCNHDNSVVCTLDKKNSIGLLECKKCNLSFQAPINSLSQPIDIYSDWIDAC EAVAEENADVNGDNFIENDGADREQDDDYDDEF C4QZ45 Transcription elongation factor SPT4 Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.012039613999999999 1 Escherichia coli LEVO MRERACMLCGIVLPGRVFMQNGCPNCDSVLNLRDSDQATVNECTSSSFEGLVAVGDNEHSWVAKWLRVDRFQPGLYAVRV DGRLPSDIVAALEQYGVYYRPRDGSVID C4R0E6 Protein that forms a complex with Spt4p Komagataella phaffii (strain GS115 / ATCC 20864) GS115 / ATCC 20864 0.10145942200000001 1 Escherichia coli LEVO GPGMSETHKNQLDKVSTVSPDGPSEAVKEHSLQSKDLSKNDGQFIVPLDRNVIEQEEHKQVKSSAQAHNTTGDAADNEIE DGVPSEDVEFDKFKEDDYDEDDEVEEEGDIRSRKRRRHNQFLDVEAEVDDEEDDDDDDDDVELKHDFIQDDHIQHETQNE GFIAGHVDDDRLHRKLDQSREKIADQDAQELADEFKQRYGRSASSKYMGSASTTAPQRLLIPTVDDPGIWGVKVRLGKEK DVVRQILKKKLAREGTKNPLEIYSAFQRDSFKGHVYIEARKAEAINDALKGNVNVFSNNSKFLVGIVEYKDLLRPVKSSD VKLTRGSYVRVKNGKFKGDLAQVDEVLENGLEARLKLVPRLDYGKDLSHLSTSSSVDSTKNRRKFYTSKFRPAQRLFSEA EARVHEPTIRRDRDGFVTYGGEEYYEGFLYKTFRLQNLIVNSINPTLNELSLFQSNEESTTIDLSTIADSLKETAKNLVS FQPGDNVEIINGELNHLTGTVSSVNQSTIVSVRLHSDDDTINSETVEIPTSDLRKIFNVGDHVRVIHGKHTDDTGLIVEV NGDKVEFISNQTKRTVIVFSNYLIKSTDSTVSINESGRFELHDLVQVNSDLVGIVIRAQKDSFDVLCSDGKLLSLPPVSI YSKLNLNPNQQIAIDSNGVEVKVGDTVREFTGERRQGTILHVYRNFLFLRSREIVENQGVFVTSSNRVKTITSKSNGTGG QISGPDLSRMNPSRVIPPPSIPVANQRMTGRDPTLNKTVKIRQGGYKGKIGIVKEANGDRFRVELHNPNKTIPIPCSFLL IESTHGWVPYEDFVASDRRGGNIPRHEISGHVQQPQHGRAPAWGSGGKTPAWGSGGKTPAWGSGGSGGKTPAWGSGGKTP TWGSGAKTPAWGSGSKTPAWSGLDEEDRRDF C4R370 Histone H3.3 Homo sapiens 0.015643262 2 Escherichia coli LEVO GSHMARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQ DFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA P84243 Histone H4 Homo sapiens 0.011676702999999998 2 Escherichia coli LEVO GSHMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHA KRKTVTAMDVVYALKRQGRTLYGFGG P62805 Histone H2A type 1-B/E Homo sapiens 0.014447825 2 Escherichia coli LEVO GSHMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKT RIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK P04908 Histone H2B type 1-J Homo sapiens 0.014217516 2 Escherichia coli LEVO GSHMPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGE ASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK P06899 DNA (36-MER) synthetic construct 0.011053189 1 (DT)(DA)(DC)(DA)(DC)(DC)(DC)(DA)(DA)(DG)(DA)(DC)(DA)(DC)(DC)(DA)(DG)(DG)(DC)(DA) (DC)(DG)(DA)(DG)(DA)(DC)(DA)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC) DNA DNA (36-MER) synthetic construct 0.011100073 1 (DG)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DG)(DT)(DC)(DT)(DC)(DG)(DT)(DG)(DC)(DC) (DT)(DG)(DG)(DT)(DG)(DT)(DC)(DT)(DT)(DG)(DG)(DG)(DT)(DG)(DT)(DA) DNA ZINC ION 6.5409e-05 10 ZN MAGNESIUM ION 2.4305e-05 1 MG singleParticle particle 7.5 ETHANE FEI TECNAI ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 50.0 1 6A5T C1 4.2 FSC 0.143 CUT-OFF RELION 3.0-beta2 31784 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_0676_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 357.6 357.6 357.6 90.0 90.0 90.0 X Y Z -0.028083773 0.08143765 -0.000020022786 0.0035535386 1.49 1.49 1.49 AUTHOR ::::EMDATABANK.org::::EMD-0676:::: RNAP reconstruction emd_0676_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 0 0 0 240 240 240 357.6 357.6 357.6 90.0 90.0 90.0 X Y Z -0.019283071 0.067641415 -0.00026334394 0.0021388133 1.49 1.49 1.49 AUTHOR ::::EMDATABANK.org::::EMD-0676:::: Nucleosome reconstruction (postprocessed)