2019-06-19 REL RCSB RCSB RCSB 2019-03-09 2019-04-10 2019-04-24 2019-06-19 National Institutes of Health/National Human Genome Research Institute 5R01GM115882-03 United States Other private David and Lucille Packard Foundation United States Structural basis of Dot1L stimulation by histone H2B lysine 120 ubiquitination. 5.2A reconstruction of Dot1L on H2BK120Ub nucleosome Valencia-Sanchez MI De Ioannes P Wang M Vasilyev N Chen R Nudler E Armache J-P Armache K-J Valencia-Sanchez MI De Ioannes P Wang M Vasilyev N Chen R Nudler E Armache JP Armache KJ Structural Basis of Dot1L Stimulation by Histone H2B Lysine 120 Ubiquitination. Mol.Cell US 74 1010 1019.e6 2019 30981630 doi:10.1016/j.molcel.2019.03.029 1097-2765 2168 MOCEFL EMD-0654 associated EM volume Cryo-EM structure of human Dot1L bound to H2BK120Ub nucleosome at 5.2A resolution Cryo-EM structure of human Dot1L bound to H2BK120Ub nucleosome at 5.2A resolution 0 Homo sapiens Escherichia coli BL21(DE3) singleParticle particle 7.5 CARBON HOLEY
unspecified
 ETHANE 100 295.15 FEI VITROBOT MARK I
3 ul of Dot1L-nucleosome complexes were applied to a glow discharged Quantifoil holey carbon grid (1.2 um hole size, 200 mesh), blotted in a Vitrobot Mark III (FEI Company) using 1.5 seconds blotting at 100% humidity, and then plunge-frozen in liquid ethane cooled by liquid nitrogen..
This sample was monodisperse
 FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 5.0 41.0 1
Frames were motion corrected using MotionCor2 with dose-weighting
1607487 Gctf Generated using cryosparc ab initio run 5.2 FSC 0.143 CUT-OFF 85029 PROJECTION MATCHING PROJECTION MATCHING emd_0654.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 371.2 371.2 371.2 90.0 90.0 90.0 X Y Z -7.5235167 13.706490499999999 -0.0060144644 0.40734196 1.45 1.45 1.45 2.0 AUTHOR Result of cisTEM refinement, filtered to 5.2A, not sharpened. This map is in the same environment as the model constructed. It is shifted and resampled to fit with the environment of the paper FLEXIBLE FIT REAL emd_0654_additional.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 371.2 371.2 371.2 90.0 90.0 90.0 X Y Z -8.098045000000001 14.464136 -0.006739371 0.43449485 1.45 1.45 1.45 AUTHOR Result of cisTEM refinement, filtered to 5.2A, not sharpened. This map is not in the same environment as the model emd_0654_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 371.2 371.2 371.2 90.0 90.0 90.0 X Y Z -6.398507 10.823779999999999 -0.10943525 0.3357682 1.45 1.45 1.45 AUTHOR Half-map 2 emd_0654_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 371.2 371.2 371.2 90.0 90.0 90.0 X Y Z -6.8758082 11.210794999999999 -0.10947102 0.335982 1.45 1.45 1.45 AUTHOR Half-map 1