2025-05-28 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name OTHER atom_sites _atom_sites.fract_transf_matrix[1][1] _atom_sites.fract_transf_matrix[2][2] _atom_sites.fract_transf_matrix[3][3] DATABASE_REFERENCES chem_comp_atom chem_comp_bond database_2 _database_2.pdbx_DOI _database_2.pdbx_database_accession STRUCTURE_SUMMARY em_admin em_software pdbx_entry_details _em_admin.last_update _em_software.name RCSB RCSB 2019-03-08 2019-04-24 2019-04-24 2025-05-28 National Institutes of Health/National Center for Research Resources (NIH/NCRR) GM079179 United States Howard Hughes Medical Institute (HHMI) United States Other private the Cancer Prevention and Research Initiative of Texas United States Other private Murchison Linthicum Scholar in Medical Research fund United States Welch Foundation I-1578 United States Cryo-EM structure of OTOP3 from xenopus tropicalis Chen QF Bai XC Otopetrin, proton, channel, MEMBRANE PROTEIN Chen Q Zeng W She J Bai X Jiang Y Structural and functional characterization of an otopetrin family proton channel. Elife US 8 2019 30973323 doi:10.7554/eLife.46710 2050-084X 6o84 FULLOVERLAP Xenopus tropicalis OTOP3 Xenopus tropicalis OTOP3 0 1 Xenopus tropicalis 0.077 LOC100127796 protein,LOC100127796 protein,OTOP3,LOC100127796 protein,LOC100127796 protein Xenopus tropicalis 0.073352297 2 Homo sapiens LEVO MLSKEEPACRQFHSREKTWGNEHNGKTVTQANNKEHKVPAVRRGDIGQREPAAHPASHIQGTMASENTGEQATCKDQYMD LDAPGNNLEHSWLHRHCEIPTTLHQRAKKTGRLFSGLFGLNLMFLGGTVVSSVALSNKAVPERDSQSFLCILMLLSSVWA LYHLLFIRNQNGAVHHDHHAGAMWLKASLAIFGVCSIILSIFEIGHALLLQNCEILMDIVFFSIEIVFVSVQTVLLWVSC KDCVQMHHSVTRYGIMLTLATDILLWLTAVIDDSLEQDLEILQSNSTQDESNEMAQCQCPTDSMCWGLKQGYVTMFPFNI EYSLICATLLFIMWKNVGRRE(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)W(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)RKLDVTLLFVSAVGQLGISYFSIIATVVTTPWTML SALNFSNSLLLILQYLSQTMFIIESMRSIHEEEKEKPGHHEESHRRMSVQEMHKAPPSCLDAGHLGLSRRVVKEMAMFLM ICNIMCWILGAFGAHPLYMNGLERQLYGSGIWLAILNIGLPLSVFYRMHSVGILLEVYLHALEGSSLVPR A9JTM7 A9JTM7 singleParticle particle 1.3 8.0 150.0 NaCl sodium chloride
Solutions were made fresh from stock solutions and filtered.
 ETHANE 100 277 FEI VITROBOT MARK IV
This sample was monodisperse
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 1.2 3.0 46730.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 1 3000 15.0 50.0 1
30 frames per movie stack were saved for motion correction.
1433949
The particles were auto-picked in RELION.
 Gctf
The CTF correction was performed during the map refinement in RELION.
PHASE FLIPPING AND AMPLITUDE CORRECTION 3.92 FSC 0.143 CUT-OFF RELION 2 212551 OTHER RELION 2 PROJECTION MATCHING RELION 2
Auto-refinement in RELION generated the final reconstruction. The angular sampling was determined automatically in RELION.
 8 RELION 2 emd_0650.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 192.6 192.6 192.6 90.0 90.0 90.0 X Y Z -0.05631465 0.11147861 -0.000003469417 0.005685513 1.07 1.07 1.07 0.03 AUTHOR Cryo-EM structure of OTOP3 from xenopus tropicalis