2020-08-12 REL RCSB RCSB RCSB 2019-03-04 2019-03-27 2019-06-05 2020-08-12 National Institutes of Health/National Institute of General Medical Sciences R01GM112050 United States National Institutes of Health/National Institute of General Medical Sciences R01GM055667 United States National Institutes of Health/National Institute of General Medical Sciences R01GM083122 United States The axonemal doublet microtubule focusing on the inner junction region extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas wild type cilia Lin J Fu G Nicastro D Dymek EE Porter M Smith EF Dymek EE Lin J Fu G Porter ME Nicastro D Smith EF PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility. Mol.Biol.Cell US 30 1805 1816 2019 31116684 doi:10.1091/mbc.E19-01-0063 1939-4586 EMD-0628 associated EM volume EMD-0629 other EM volume EMD-0630 other EM volume The inner junction region of axonemal doublet microtubule averaged from Chlamydomonas wild type cilia The inner junction region of axonemal doublet microtubule averaged from Chlamydomonas wild type cilia 0 Chlamydomonas reinhardtii cilia subtomogramAveraging cell 7.4 Quantifoil R2/2 COPPER 200 CARBON HOLEY GLOW DISCHARGE AIR 101.325 ETHANE 298 HOMEMADE PLUNGER
back-side blotting for 1.5-2.5 seconds before plunging.
Freshly isolated and demembranated cilia from Chlamydomonas wild type cells
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 0.2 0.5 26000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE GIF Bioquantum 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 3710 3838 1-15 6.0 1.57 1 C1 BACK PROJECTION 23.0 FSC 0.5 CUT-OFF IMOD 2381 19 2381 MATLAB OTHER IMOD emd_0628.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 80 70 95 -14 -73 -21 80 70 95 441.83997 386.61 524.685 90.0 90.0 90.0 X Y Z 116.72943 137.5144 125.730934 4.1222625 5.523 5.523 5.523 128 AUTHOR The doublet microtubule focusing on the inner junction region of Chlamydomonas wild type cilia