2025-05-28 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_software _em_admin.last_update _em_software.name DATABASE_REFERENCES citation _citation.journal_volume _citation.page_first _citation.page_last AUTHOR_SUPPORTING_EVIDENCE pdbx_audit_support _pdbx_audit_support.funding_organization OTHER atom_sites _atom_sites.fract_transf_matrix[1][1] _atom_sites.fract_transf_matrix[2][2] _atom_sites.fract_transf_matrix[3][3] DATABASE_REFERENCES chem_comp_atom chem_comp_bond database_2 _database_2.pdbx_DOI _database_2.pdbx_database_accession STRUCTURE_SUMMARY em_admin em_software pdbx_entry_details _em_admin.last_update _em_software.name RCSB RCSB 2019-02-17 2019-05-22 2019-07-10 2025-05-28 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 097042 United States National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD) 081534 United States National Institutes of Health/National Cancer Institute (NIH/NCI) United States Zhu X Clarke R CRISPR, Cas9, Nuclease, Genome editing, HYDROLASE, HYDROLASE-RNA-DNA complex Zhu X Clarke R Puppala AK Chittori S Merk A Merrill BJ Simonovic M Subramaniam S Nat.Struct.Mol.Biol. US 26 679 685 2019 31285607 doi:10.1038/s41594-019-0258-2 1545-9985 6o0y FULLOVERLAP Cas9-sgRNA-DNA ternary complex Cas9-sgRNA-DNA ternary complex 0 1 2 3 4 5 Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Streptococcus pyogenes 0.158986109 1 Escherichia coli LEVO GAASMDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRK NRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYL ALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNG LFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAP LSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNRE DLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPW NFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTN RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQ GDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQIL KEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVV KKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVI TLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKY FFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNS DKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLY ETRIDLSQLGGD Q99ZW2 3.1.-.- single guide RNA synthetic construct 0.033059699 1 GGGGCGCAUAAAGAUGAGACGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAA GUGGCACCGAGUCGGUGCUUGC 5' product of target strand DNA synthetic construct 0.0039365749999999994 1 (DC)(DT)(DA)(DA)(DT)(DC)(DG)(DC)(DC)(DA)(DG)(DC)(DG) DNA non-target strand DNA synthetic construct 0.012452000999999999 1 (DC)(DC)(DA)(DG)(DT)(DG)(DC)(DG)(DT)(DA)(DG)(DG)(DC)(DG)(DC)(DA)(DT)(DA)(DA)(DA) (DG)(DA)(DT)(DG)(DA)(DG)(DA)(DC)(DG)(DC)(DT)(DG)(DG)(DC)(DG)(DA)(DT)(DT)(DA)(DG) DNA 3' product of target strand DNA synthetic construct 0.008194269 1 (DT)(DC)(DT)(DC)(DA)(DT)(DC)(DT)(DT)(DT)(DA)(DT)(DG)(DC)(DG)(DC)(DC)(DT)(DA)(DC) (DG)(DC)(DA)(DC)(DT)(DG)(DG) DNA singleParticle particle 0.5 7.5 PLASMA CLEANING

unspecified

ETHANE LEICA EM GP FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) 73.0 1 NONE C1 3.37 FSC 0.143 CUT-OFF 27433 RANDOM ASSIGNMENT MAXIMUM LIKELIHOOD