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    <admin>
        <current_status>
            <date>2024-03-20</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-02-15</deposition>
            <header_release>2019-03-13</header_release>
            <map_release>2019-06-12</map_release>
            <update>2024-03-20</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Academy of Finland</funding_body>
                <code>275199</code>
                <country>Finland</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Academy of Finland</funding_body>
                <code>315950</code>
                <country>Finland</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Sigrid Juselius Foundation</funding_body>
                <country>Finland</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Jane and Aatos Erkko Foundation</funding_body>
                <country>Finland</country>
            </grant_reference>
        </grant_support>
        <title>Extracellular factors prime enterovirus particles for uncoating</title>
        <authors_list>
            <author>Domanska A</author>
            <author>Ruokolainen V</author>
        </authors_list>
        <keywords>expanded particle, VIRUS</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Ruokolainen V</author>
                    <author ORCID="0000-0002-3772-6003" order="2">Domanska A</author>
                    <author order="3">Laajala M</author>
                    <author order="4">Pelliccia M</author>
                    <author ORCID="0000-0001-7060-5871" order="5">Butcher SJ</author>
                    <author order="6">Marjomaki V</author>
                    <title>Extracellular Albumin and Endosomal Ions Prime Enterovirus Particles for Uncoating That Can Be Prevented by Fatty Acid Saturation.</title>
                    <journal_abbreviation>J.Virol.</journal_abbreviation>
                    <country>US</country>
                    <volume>93</volume>
                    <year>2019</year>
                    <external_references type="PUBMED">31189702</external_references>
                    <external_references type="DOI">doi:10.1128/JVI.00599-19</external_references>
                    <external_references type="ISSN">1098-5514</external_references>
                    <external_references type="CSD">0825</external_references>
                    <external_references type="ASTM">JOVIAM</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6o06</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Echovirus E1</name>
        <supramolecule_list>
            <virus_supramolecule supramolecule_id="1">
                <name>Echovirus E1</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <details>Echovirus 1 was purified from infected GMK cells</details>
                <sci_species_name ncbi="46633">Echovirus E1</sci_species_name>
                <natural_host database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_host>
                <virus_shell shell_id="1">
                    <name>icosahedral</name>
                    <diameter units="Å">300.0</diameter>
                    <triangulation>1</triangulation>
                </virus_shell>
                <virus_type>VIRION</virus_type>
                <virus_isolate>OTHER</virus_isolate>
                <virus_enveloped>false</virus_enveloped>
                <virus_empty>false</virus_empty>
            </virus_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>VP1</name>
                <natural_source database="NCBI">
                    <organism ncbi="46633">Echovirus E1</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.031604373</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GDVQNAVEGAMVRVADTVQTSATNSERVPNLTAVETGHTSQAVPGDTMQTRHVINNHVRSESTIENFLARSACVFYLEYK
TGTKEDSNSFNNWVITTRRVAQLRRKLEMFTYLRFDMEITVVITSSQDQSTSQNQNAPVLTHQIMYVPPGGPIPVSVDDY
SWQTSTNPSIFWTEGNAPARMSIPFISIGNAYSNFYDGWSHFSQAGVYGFTTLNNMGQLFFRHVNKPNPAAITSVARIYF
KPKHVRAWVPRPPRLCPYINSTNVNFEPKPVTEVRTNIITT</string>
                    <external_references type="UNIPROTKB">O91734</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>VP2</name>
                <natural_source database="NCBI">
                    <organism ncbi="46633">Echovirus E1</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.028126465</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GYSDRVRSITLGNSTITTQECANVVVGYGEWPEYLSDNEATAEDQPTQPDVATCRFYTLDSVQWENGSPGWWWKFPDALR
DMGLFGQNMYYHYLGRAGYTIHVQCNASKFHQGCILVVCVPEAEMGSAQTSGVVNYEHISKGEIASRFTTTTTAEDHGVQ
AAVWNAGMGVGVGNLTIFPHQWINLRTNNSATIVMPYVNSVPMDNMYRHHNFTLMIIPFVPLDFSAGASTYVPITVTVAP
MCAEYNGLRLAGHQ</string>
                    <external_references type="UNIPROTKB">O91734</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>VP3</name>
                <natural_source database="NCBI">
                    <organism ncbi="46633">Echovirus E1</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.026471074</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>GLPTMNTPGSNQFLTSDDFQSPSAMPQFDVTPEMHIPGEVRNLMEIAEVDSVMPINNDSAAKVSSMEAYRVELSTNTNAG
TQVFGFQLNPGAESVMNRTLMGEILNYYAHWSGSIKITFVFCGSAMTTGKFLLSYAPPGAGAPKTRKDAMLGTHVVWDVG
LQSSCVLCIPWISQTHYRFVEKDPYTNAGFVTCWYQTSVVSPASNQPKCYMMCMVSACNDFSVRMLRDTKFIEQTSFYQ</string>
                    <external_references type="UNIPROTKB">O91734</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.2</ph>
                        <details>29 mM sodium chloride, 28 mM potassium ion, 0.145 mM magnesium chloride, 8 mM phosphate dibasic, 2 mM phosphate monobasic, 0.0093% faf-BSA</details>
                    </buffer>
                    <grid>
                        <model>Quantifoil R2/2</model>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>HOMEMADE PLUNGER</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TALOS ARCTICA</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON III (4k x 4k)</film_or_detector_model>
                            <average_exposure_time units="s">47.8</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">30.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="OTHER"/>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>I</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.6</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                    <number_images_used>14615</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
                <final_three_d_classification>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>2.1</version>
                        </software>
                    </software_list>
                </final_three_d_classification>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="256001">
        <file>emd_0565.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>400</col>
            <row>400</row>
            <sec>400</sec>
        </dimensions>
        <origin>
            <col>-200</col>
            <row>-200</row>
            <sec>-200</sec>
        </origin>
        <spacing>
            <x>400</x>
            <y>400</y>
            <z>400</z>
        </spacing>
        <cell>
            <a units="Å">496.0</a>
            <b units="Å">496.0</b>
            <c units="Å">496.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-0.07406282</minimum>
            <maximum>0.16077633</maximum>
            <average>0.0016680729</average>
            <std>0.011457966</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.24</x>
            <y units="Å">1.24</y>
            <z units="Å">1.24</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.02</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-0565::::</label>
        <annotation_details>Echovirus 1 expanded particle sharpened map</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <initial_model>
                    <access_code>4JGY</access_code>
                    <chain>
                        <source_name>PDB</source_name>
                        <initial_model_type>experimental model</initial_model_type>
                    </chain>
                </initial_model>
                <refinement_protocol>FLEXIBLE FIT</refinement_protocol>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="256001">
                <file>emd_0565_half_map_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>-200</col>
                    <row>-200</row>
                    <sec>-200</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">496.0</a>
                    <b units="Å">496.0</b>
                    <c units="Å">496.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.023098342</minimum>
                    <maximum>0.048692286</maximum>
                    <average>0.00084238785</average>
                    <std>0.004624826</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.24</x>
                    <y units="Å">1.24</y>
                    <z units="Å">1.24</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-0565::::</label>
                <annotation_details>Echovirus 1 expanded particle half map</annotation_details>
            </half_map>
            <half_map format="CCP4" size_kbytes="256001">
                <file>emd_0565_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>400</col>
                    <row>400</row>
                    <sec>400</sec>
                </dimensions>
                <origin>
                    <col>-200</col>
                    <row>-200</row>
                    <sec>-200</sec>
                </origin>
                <spacing>
                    <x>400</x>
                    <y>400</y>
                    <z>400</z>
                </spacing>
                <cell>
                    <a units="Å">496.0</a>
                    <b units="Å">496.0</b>
                    <c units="Å">496.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.024080135</minimum>
                    <maximum>0.048774887</maximum>
                    <average>0.00082568504</average>
                    <std>0.0046312</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.24</x>
                    <y units="Å">1.24</y>
                    <z units="Å">1.24</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-0565::::</label>
                <annotation_details>Echovirus 1 expanded particle half map</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
